ID   D4L3X2_9FIRM            Unreviewed;       957 AA.
AC   D4L3X2;
DT   18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT   18-MAY-2010, sequence version 1.
DT   24-JAN-2024, entry version 61.
DE   SubName: Full=Endo-beta-N-acetylglucosaminidase D {ECO:0000313|EMBL:CBL14312.1};
GN   ORFNames=RO1_41310 {ECO:0000313|EMBL:CBL14312.1};
OS   Roseburia intestinalis XB6B4.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia.
OX   NCBI_TaxID=718255 {ECO:0000313|EMBL:CBL14312.1, ECO:0000313|Proteomes:UP000008953};
RN   [1] {ECO:0000313|EMBL:CBL14312.1, ECO:0000313|Proteomes:UP000008953}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=XB6B4 {ECO:0000313|EMBL:CBL14312.1,
RC   ECO:0000313|Proteomes:UP000008953};
RG   metaHIT consortium -- http://www.metahit.eu/;
RA   Pajon A., Turner K., Parkhill J., Bernalier A.;
RT   "The genome sequence of Roseburia intestinalis XB6B4.";
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CBL14312.1, ECO:0000313|Proteomes:UP000008953}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=XB6B4 {ECO:0000313|EMBL:CBL14312.1,
RC   ECO:0000313|Proteomes:UP000008953};
RA   Pajon A.;
RL   Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000256|ARBA:ARBA00004514}.
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DR   EMBL; FP929050; CBL14312.1; -; Genomic_DNA.
DR   AlphaFoldDB; D4L3X2; -.
DR   CAZy; CBM32; Carbohydrate-Binding Module Family 32.
DR   CAZy; GH85; Glycoside Hydrolase Family 85.
DR   KEGG; rix:RO1_41310; -.
DR   PATRIC; fig|718255.3.peg.1462; -.
DR   HOGENOM; CLU_008357_0_0_9; -.
DR   Proteomes; UP000008953; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0033925; F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd06547; GH85_ENGase; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR032979; ENGase.
DR   InterPro; IPR000421; FA58C.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005201; Glyco_hydro_85.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR022409; PKD/Chitinase_dom.
DR   InterPro; IPR000601; PKD_dom.
DR   InterPro; IPR035986; PKD_dom_sf.
DR   PANTHER; PTHR13246:SF1; CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE; 1.
DR   PANTHER; PTHR13246; ENDO BETA N-ACETYLGLUCOSAMINIDASE; 1.
DR   Pfam; PF00754; F5_F8_type_C; 1.
DR   Pfam; PF03644; Glyco_hydro_85; 1.
DR   Pfam; PF00801; PKD; 1.
DR   SMART; SM00089; PKD; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   SUPFAM; SSF49299; PKD domain; 1.
DR   PROSITE; PS50022; FA58C_3; 1.
DR   PROSITE; PS50093; PKD; 1.
PE   4: Predicted;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..38
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           39..957
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5038900551"
FT   DOMAIN          731..800
FT                   /note="PKD"
FT                   /evidence="ECO:0000259|PROSITE:PS50093"
FT   DOMAIN          808..956
FT                   /note="F5/8 type C"
FT                   /evidence="ECO:0000259|PROSITE:PS50022"
SQ   SEQUENCE   957 AA;  105181 MW;  8DB0150890D1B4AB CRC64;
     MKRRWEKKER KCFMKNKVLG RRCIAISLAA ALSAGLTACG NTGGDTQAAT TETTQITETV
     QTAERIYSLT EENENQELTM ARQPESSYWF PSELLSWDAK TDEDLRFNVS TVPLSERAAR
     EHLQTVNSTQ NKETNIMAIS IMNSSTSGNP PHGLNKVNAN TFTYWQYVDT LVYWGGSSGE
     GLIVSPSPDV VDAAHKNGVR VLGTVFMPQT AHGGKMEWLE DLLVKNEDGS YPVADKLIEV
     AQTYGFEGWF MNQETEGTDE EPLTADHAAR MQQFIQYFKE QAPDLDLVYY DSMTVDGRMD
     WQNALTEENL AYLVSEDGEP VADAMFLNFW WTSDTLSDQE LLKKSAALAS ENGINPYDLY
     AGVDIQSEGY HTEIKWDLFE NEKGGTYTSL GLYCPSWAYT SADTIQNFWK QENKLWVNSM
     GDPSADVKKL SNTQWKGISS YIVERTPLTS LPFVTNFSTG NGYSFFKNGS QISLLDWNNR
     SIADIMPTYR YIIENGDGNK LSADLDVADA YYGGTSLILR GNMAKDTSST IKLYAAELTA
     ADNMIYTTAA KAKGTEITLN AVLELEDGSV VTLEGDQNVG EEWTVVSYDT SSIIGKTIKS
     ISYEITSAED VSGLQLRFGN ITMMEADSEE NAAVSNLEVL DSEFDEDGMY AGVRLAWNSD
     TAADYYEVYR VNQDNSRSLL GVSNTTSFYI NTLPRTDDTN KSAFEVVPVN AAFEEGNSAQ
     VVMDWPDNSL PKADFAADVT LAAPGTKITF ESLCSANTET VNWTFARADI ETAEGESVSV
     SYPEEGIYNV KVTAVNKAGS IEKEKEGYIV ITSDAADGLV LLSQGAGTEA DAYVNENEAP
     EFAVDGDVST KWCATGAAPH EITLDLGEVN TVSAVDIFHA EAGGESADMN TKSYTILVSQ
     DGNSFEEVYS VTKNTNGTTH NAFTPKDARY VKLVVNKPTQ GSDSAARIYE IEVYGIK
//