ID D4NQ89_9INFA Unreviewed; 732 AA.
AC D4NQ89;
DT 18-MAY-2010, integrated into UniProtKB/TrEMBL.
DT 18-MAY-2010, sequence version 1.
DT 24-JAN-2024, entry version 48.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:ACY67307.1};
OS Influenza A virus (A/northern pintail/Alaska/44344-290/2007(H10N7)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=688675 {ECO:0000313|EMBL:ACY67307.1};
RN [1] {ECO:0000313|EMBL:ACY67307.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/northern pintail/Alaska/44344-290/2007
RC {ECO:0000313|EMBL:ACY67307.1};
RX PubMed=20227102; DOI=10.1016/j.virol.2010.02.006;
RA Ramey A.M., Pearce J.M., Flint P.L., Ip H.S., Derksen D.V., Franson J.C.,
RA Petrula M.J., Scotton B.D., Sowl K.M., Wege M.L., Trust K.A.;
RT "Intercontinental reassortment and genomic variation of low pathogenic
RT avian influenza viruses isolated from northern pintails (Anas acuta) in
RT Alaska: examining the evidence through space and time.";
RL Virology 401:179-189(2010).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; GU168179; ACY67307.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 4: Predicted;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 269..466
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 33..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACY67307.1"
FT NON_TER 732
FT /evidence="ECO:0000313|EMBL:ACY67307.1"
SQ SEQUENCE 732 AA; 83514 MW; D4EFB13EC4E65417 CRC64;
ISTTFPYTGD PPYSHGTGTG YTMDTVNRTH QYSEKGKWTT NTETGAPQLN PIDGPLPEDN
EPSGYAQTDC VLEAMAFLEE SHPGIFENSC LETMEVVQQT RVDKLTQGRQ TYDWTLNRNQ
PAATALANTI EVFRSNGLTA NESGRLIDFL KDVMESMDKE EIEITTHFQR KRRVRDNMTK
KMVTQRTIGK KKQRLNKRGY LIRALTLNTM TKDAERGKLK RRAIATPGMQ IRGFVYFVET
LARSICEKLE QSGLPVGGNE KKAKLANVVR KMMTNSQDTE LSFTITGDNT KWNENQNPRM
FLAMITYITR NQPEWFRNVL SIAPIMFSNK MARLGKGYMF ESKSMKLRTQ IPAEMLASID
LKYFNESTRK KIERIRPLLI DGTASLSPGM MMGMFNMLST VLGVSILNLG QKRYTKTTYW
WDGLQSSDDF ALIVNAPNHE GIQAGVDRFY RTCKLVGINM SKKKSYINRT GTFEFTSFFY
RYGFVANFSM ELPSFGVSGI NESADMSIGI TVIKNNMINN DLGPATAQMA LQLFIKDYRY
TYRCHRGDTQ IQTRRSFELK KLWEQTRSKA GLLVSDGGPN LYNIRNLHIP EVCLKWELMD
EDYQGRLCNP LNPFVSHKEI ESVNNAVVMP AHGPAKSMEY DAVATTHSWI PKRNRSILNT
SQRGILEDEQ MYQKCCNLFE KFFPSSSYRR PVGISSMVEA MVSRARIDAR IDFESGRIKK
EEFAEIMKIC ST
//
