ID D4QGR7_9GENT Unreviewed; 350 AA.
AC D4QGR7;
DT 15-JUN-2010, integrated into UniProtKB/TrEMBL.
DT 15-JUN-2010, sequence version 1.
DT 08-NOV-2023, entry version 45.
DE SubName: Full=O-methyltransferase {ECO:0000313|EMBL:BAJ05387.1};
GN Name=IpeOMT8 {ECO:0000313|EMBL:BAJ05387.1};
OS Carapichea ipecacuanha.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Rubiaceae; Rubioideae; Palicoureeae;
OC Carapichea.
OX NCBI_TaxID=77880 {ECO:0000313|EMBL:BAJ05387.1};
RN [1] {ECO:0000313|EMBL:BAJ05387.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Root {ECO:0000313|EMBL:BAJ05387.1};
RX PubMed=20061395; DOI=10.1074/jbc.M109.086157;
RA Nomura T., Kutchan T.M.;
RT "Three new O-methyltransferases are sufficient for all O-methylation
RT reactions of ipecac alkaloid biosynthesis in root culture of Psychotria
RT ipecacuanha.";
RL J. Biol. Chem. 285:7722-7738(2010).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Cation-independent O-methyltransferase family. COMT
CC subfamily. {ECO:0000256|ARBA:ARBA00034481}.
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DR EMBL; AB556942; BAJ05387.1; -; mRNA.
DR AlphaFoldDB; D4QGR7; -.
DR GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR016461; COMT-like.
DR InterPro; IPR001077; O_MeTrfase_dom.
DR InterPro; IPR012967; Plant_MeTrfase_dimerisation.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11746; O-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11746:SF286; OS01G0753300 PROTEIN; 1.
DR Pfam; PF08100; Dimerisation; 1.
DR Pfam; PF00891; Methyltransf_2; 1.
DR PIRSF; PIRSF005739; O-mtase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51683; SAM_OMT_II; 1.
PE 2: Evidence at transcript level;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:BAJ05387.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000313|EMBL:BAJ05387.1}.
FT DOMAIN 31..78
FT /note="Plant methyltransferase dimerisation"
FT /evidence="ECO:0000259|Pfam:PF08100"
FT DOMAIN 123..332
FT /note="O-methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF00891"
FT ACT_SITE 254
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005739-1"
SQ SEQUENCE 350 AA; 39050 MW; A642750EBFDED975 CRC64;
METVESSSSA ELLRAQTHFS TQLFSFQNYA SLKCALHLGI PDAIKQHGKP MNLSELTSAL
PINPSKAPYI HRLMRMLVKA GYFAQENECF DLTPIGLLLL KDDPINIRAL VLLELHPALL
MPWIALSEWF QNDDATPFVT AHGKSFWDYT SRDPEFRKLF DEAMAGDSEL ISKVLVTEFK
YVFEGLKSLV DVGGGNGTLA RSIAKAFPNL KCTVFDLPES VANEQGDGNL DFVAGDMFDR
VPSADAILLK IILHDWSDEN CVKILKNCKK SIPGKDKGGK VIIIEGVVEL EKNAGNEYAG
LENLDMEMMV LYNSKERTKK EWAKLFSDAG FSDYKFIPAL DSWCIIELSP
//