ID D5X0W0_THIK1 Unreviewed; 738 AA.
AC D5X0W0;
DT 13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT 13-JUL-2010, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE SubName: Full=(P)ppGpp synthetase I, SpoT/RelA {ECO:0000313|EMBL:ADG30756.1};
DE EC=2.7.6.5 {ECO:0000313|EMBL:ADG30756.1};
GN OrderedLocusNames=Tint_1369 {ECO:0000313|EMBL:ADG30756.1};
OS Thiomonas intermedia (strain K12) (Thiobacillus intermedius).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales; Thiomonas.
OX NCBI_TaxID=75379 {ECO:0000313|EMBL:ADG30756.1};
RN [1] {ECO:0000313|EMBL:ADG30756.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 {ECO:0000313|EMBL:ADG30756.1};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M.,
RA Hauser L., Kyrpides N., Ovchinnikova G., Kerfeld C.A., Cannon G.C.,
RA Heinhorst S., Woyke T.;
RT "Complete sequence of Thiomonas intermedia K12.";
RL Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate)
CC is a mediator of the stringent response that coordinates a variety of
CC cellular activities in response to changes in nutritional abundance.
CC {ECO:0000256|RuleBase:RU003847}.
CC -!- SIMILARITY: Belongs to the relA/spoT family.
CC {ECO:0000256|RuleBase:RU003847}.
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DR EMBL; CP002021; ADG30756.1; -; Genomic_DNA.
DR AlphaFoldDB; D5X0W0; -.
DR STRING; 75379.Tint_1369; -.
DR KEGG; tin:Tint_1369; -.
DR eggNOG; COG0317; Bacteria.
DR HOGENOM; CLU_012300_3_0_4; -.
DR BioCyc; TINT75379:TINT_RS06860-MONOMER; -.
DR GO; GO:0008728; F:GTP diphosphokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0015969; P:guanosine tetraphosphate metabolic process; IEA:InterPro.
DR CDD; cd04876; ACT_RelA-SpoT; 1.
DR CDD; cd05399; NT_Rel-Spo_like; 1.
DR CDD; cd01668; TGS_RSH; 1.
DR Gene3D; 3.10.20.30; -; 1.
DR Gene3D; 3.30.70.260; -; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 1.10.3210.10; Hypothetical protein af1432; 1.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR012675; Beta-grasp_dom_sf.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR004811; RelA/Spo_fam.
DR InterPro; IPR007685; RelA_SpoT.
DR InterPro; IPR004095; TGS.
DR InterPro; IPR012676; TGS-like.
DR InterPro; IPR033655; TGS_RelA/SpoT.
DR NCBIfam; TIGR00691; spoT_relA; 1.
DR PANTHER; PTHR21262:SF31; BIFUNCTIONAL (P)PPGPP SYNTHASE_HYDROLASE SPOT; 1.
DR PANTHER; PTHR21262; GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASE; 1.
DR Pfam; PF13291; ACT_4; 1.
DR Pfam; PF13328; HD_4; 1.
DR Pfam; PF04607; RelA_SpoT; 1.
DR Pfam; PF02824; TGS; 1.
DR SMART; SM00954; RelA_SpoT; 1.
DR SUPFAM; SSF55021; ACT-like; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81271; TGS-like; 1.
DR PROSITE; PS51671; ACT; 1.
DR PROSITE; PS51880; TGS; 1.
PE 3: Inferred from homology;
KW Transferase {ECO:0000313|EMBL:ADG30756.1}.
FT DOMAIN 408..471
FT /note="TGS"
FT /evidence="ECO:0000259|PROSITE:PS51880"
FT DOMAIN 667..738
FT /note="ACT"
FT /evidence="ECO:0000259|PROSITE:PS51671"
SQ SEQUENCE 738 AA; 80751 MW; 4202A0B888658BD9 CRC64;
MSPNQSVTVR SAAQAEPADQ RIDQARAYAL ALIDTVVLET GEPAVEHADG VSAILREIGA
DADAQAAAYL NLVVPQLAHP QDQLSKHFGE ALAALSVETR KLVEVSRMAR GGPDEAELRR
RHTELVRRLL LGFSRDLRVA LLRLASRLQS LRYFTRTKQA APAAFLAESM EVYAPLANRL
GIWQVKWEME DLSFRLMQPE VYKDIARKLD ERRVEREQGI STAISLLQKE LQRQHIAAEV
VGRPKHIYSI WRKMQGKHLQ FEQVMDLRAL RIIVGDVSAC YAALSVVHAM WASLPGEFDD
YIAKPKPNGY QSLHTVVRTD SGQIFEIQIR TAAMHAHAEQ GSAAHWAYKE AGAKGYLGQT
QAGDYQSRIA LARQLLALQQ ELAGASSPAA NAPASTGASA DPAVEADAED RIFVLTPQAR
IVELARGATP VDFAYALHTD LGHRCRGARV DGALIPLTTP LRSGQTVEIV AAKEGGPSRD
WLNAELGYLR SPRARAKVRA WFNQLAQQQT IAKGRAQVER LLQREGKTSF NLQSLAEGLG
FRTADELFER VGKDEFSQRQ IEAYLRADPA TSAADESLVF SSGGAAPKKQ GGQVLVVGMG
SLLTQLAACC KPAPPDAIGG FVTREKGVTV HRANCQNFRN IARQHPERVI AVTWAGQDDA
SALYPIDLEV SATDRQGLLR DISDVLTQER INVIAVSTRS VGDLAQMRFT VQLANSAKLK
RALKLIADVP GVLKASRK
//
