ID D6XWN8_BACIE Unreviewed; 320 AA.
AC D6XWN8;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE SubName: Full=Alpha-N-arabinofuranosidase {ECO:0000313|EMBL:ADH97880.1};
DE EC=3.2.1.55 {ECO:0000313|EMBL:ADH97880.1};
GN OrderedLocusNames=Bsel_0340 {ECO:0000313|EMBL:ADH97880.1};
OS Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
OC Salisediminibacterium.
OX NCBI_TaxID=439292 {ECO:0000313|EMBL:ADH97880.1, ECO:0000313|Proteomes:UP000000271};
RN [1] {ECO:0000313|EMBL:ADH97880.1, ECO:0000313|Proteomes:UP000000271}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700615 / DSM 15326 / MLS10
RC {ECO:0000313|Proteomes:UP000000271};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Stolz J.;
RT "Complete sequence of Bacillus selenitireducens MLS10.";
RL Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR EMBL; CP001791; ADH97880.1; -; Genomic_DNA.
DR RefSeq; WP_013171309.1; NC_014219.1.
DR AlphaFoldDB; D6XWN8; -.
DR STRING; 439292.Bsel_0340; -.
DR CAZy; GH43; Glycoside Hydrolase Family 43.
DR KEGG; bse:Bsel_0340; -.
DR eggNOG; COG3940; Bacteria.
DR HOGENOM; CLU_009397_2_1_9; -.
DR OrthoDB; 177947at2; -.
DR Proteomes; UP000000271; Chromosome.
DR GO; GO:0046556; F:alpha-L-arabinofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18817; GH43f_LbAraf43-like; 1.
DR InterPro; IPR016828; Alpha-L-arabinofuranosidase.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43817; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR43817:SF1; HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR PIRSF; PIRSF025414; Alpha-L-arabinofuranosidase; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187, ECO:0000313|EMBL:ADH97880.1};
KW Hydrolase {ECO:0000256|RuleBase:RU361187, ECO:0000313|EMBL:ADH97880.1}.
FT ACT_SITE 17
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 197
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 136
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 320 AA; 37058 MW; 86FA28CAE6CB546F CRC64;
MTQSYDYKNP VIEQRADPWM YKHTDGYYYF TASVPEYDRI EVRRAKTIEG LKGATPAVVW
RKYDEGPLSA NIWAPEIHYI DGAWYIYFAA ARTTETDNGL FDHRMYALAC QSNNPVEGTW
EEKGQVKSKW ESFCLDATVF EHKGDHYYVW AQKDPDIEGN SNMYIDKMVN PWTLKGDQVM
ISQPEYEWEK VGFYVNEGAA VIKRNGKIFM TFSGSATDHN YRMGLLYADE ESDLLNPSSW
HKLDKPVFAT SEENGQYGPG HNSFTTSEDG EKDLLVYHAR NYKEIDGDPL YDPNRHTRVQ
AFEWDENGFP VFEKPVPDAD
//
