ID D7E836_METEZ Unreviewed; 679 AA.
AC D7E836;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=Metev_0463 {ECO:0000313|EMBL:ADI73378.1};
OS Methanohalobium evestigatum (strain ATCC BAA-1072 / DSM 3721 / NBRC 107634
OS / OCM 161 / Z-7303).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanohalobium.
OX NCBI_TaxID=644295 {ECO:0000313|EMBL:ADI73378.1, ECO:0000313|Proteomes:UP000000391};
RN [1] {ECO:0000313|EMBL:ADI73378.1, ECO:0000313|Proteomes:UP000000391}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1072 / DSM 3721 / NBRC 107634 / OCM 161 / Z-7303
RC {ECO:0000313|Proteomes:UP000000391};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Saunders E., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Anderson I.,
RA Woyke T.;
RT "Complete sequence chromosome of Methanohalobium evestigatum Z-7303.";
RL Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP002069; ADI73378.1; -; Genomic_DNA.
DR RefSeq; WP_013193946.1; NC_014253.1.
DR AlphaFoldDB; D7E836; -.
DR STRING; 644295.Metev_0463; -.
DR REBASE; 26597; M.MevORF463P.
DR GeneID; 9346082; -.
DR KEGG; mev:Metev_0463; -.
DR HOGENOM; CLU_012122_0_0_2; -.
DR OrthoDB; 45790at2157; -.
DR Proteomes; UP000000391; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ADI73378.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000391};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 8..140
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 152..454
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 679 AA; 79473 MW; 45D5BD950BB7FF4E CRC64;
MSNFQEKANF IWSVADEVLR DDFKRGKYRD VILPFTVLRR VECVLEPTKD NVIQTYENVK
DKVKDPHNAL CHAAGHSFYN TSPYDLKKLL DDPSNIGQNF KSYINSFSEN MRDIFDKFYL
WNYIDQLIED NLLYMLLEKF SNVDLHPDSV SNHEMGYIFE ELIRRFNEDV NENPGEHFTP
REVIRLMVNL IFYQDEAKLG HNTPIRTIYD PACGTGGMLT IANDHILKEI NSNADIWLFG
QEVNPETFAI AKSDMMLKGN DRDAENIKMG SVFSNDGHPN ETFNYMLSNP PFGKDWKKEQ
NFILEEAKSA SSRFTAGLPR KDDGQLLFLQ HMISKMKRPE DGGSRIAVVT NGSPLFTGDA
GSGESEIRRW IIENDWLEAI VALPEQLFYN TGINTYVWIV TNRKEDHRKG KIQLVDAREC
YQKMRKSLGE KRHEISSEQI DTITNLHNNF NEGQYSQIFD NHEFGYRKIT IEQPLRLSFQ
VTPERIEQLK EQKAFKNLAV SKKRKNTEEK EKEEAEGQKL QDSIIEMLSE MDSEKFYKNR
DEFWKVLNDN LKKHGININN TVEKAILDSM SERDETADIC VDSKKRWEPD SQLRDYENVP
LDEDIYDYFE REVKPHVPEA WIDESKTDQY DNDVGKVGYI INFNRYFYEY EPPRPLEEIE
SDINDLENEI LELLQEVSH
//
