ID D7F7W8_ONCMY Unreviewed; 1002 AA.
AC D7F7W8;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN Name=mda5 {ECO:0000313|EMBL:CAZ27715.1};
OS Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC Salmonidae; Salmoninae; Oncorhynchus.
OX NCBI_TaxID=8022 {ECO:0000313|EMBL:CAZ27715.1};
RN [1] {ECO:0000313|EMBL:CAZ27715.1}
RP NUCLEOTIDE SEQUENCE.
RA Chang M., Collet B., Nie P., Lester K., Campbell S., Secombes C.J., Zou J.;
RT "Expression and Functional Characterization of the RIG-I-Like Receptors
RT MDA5 and LGP2 in Rainbow Trout (Oncorhynchus mykiss).";
RL J. Virol. 85:8403-8412(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00029316};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13066;
CC Evidence={ECO:0000256|ARBA:ARBA00029316};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the helicase family. RLR subfamily.
CC {ECO:0000256|ARBA:ARBA00006866}.
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DR EMBL; FN396357; CAZ27715.1; -; mRNA.
DR RefSeq; NP_001182108.1; NM_001195179.1.
DR AlphaFoldDB; D7F7W8; -.
DR GeneID; 100499612; -.
DR KEGG; omy:100499612; -.
DR CTD; 64135; -.
DR OrthoDB; 342391at2759; -.
DR GO; GO:0005737; C:cytoplasm; IDA:AgBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003725; F:double-stranded RNA binding; IDA:AgBase.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:1902615; P:immune response involved in response to exogenous dsRNA; IMP:AgBase.
DR GO; GO:0045087; P:innate immune response; IMP:AgBase.
DR CDD; cd08818; CARD_MDA5_r1; 1.
DR CDD; cd08819; CARD_MDA5_r2; 1.
DR Gene3D; 1.20.1320.30; -; 1.
DR Gene3D; 1.10.533.10; Death Domain, Fas; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 2.170.150.30; RIG-I-like receptor, C-terminal regulatory domain; 1.
DR InterPro; IPR031964; CARD_dom.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR011029; DEATH-like_dom_sf.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041204; RIG-I-like_C.
DR InterPro; IPR038557; RLR_C_sf.
DR InterPro; IPR021673; RLR_CTR.
DR PANTHER; PTHR14074; HELICASE WITH DEATH DOMAIN-RELATED; 1.
DR PANTHER; PTHR14074:SF14; INTERFERON-INDUCED HELICASE C DOMAIN-CONTAINING PROTEIN 1; 1.
DR Pfam; PF16739; CARD_2; 2.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF18119; RIG-I_C; 1.
DR Pfam; PF11648; RIG-I_C-RD; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51789; RLR_CTR; 1.
PE 2: Evidence at transcript level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Immunity {ECO:0000256|ARBA:ARBA00022859};
KW Innate immunity {ECO:0000256|ARBA:ARBA00022588};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 313..506
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 671..839
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 864..990
FT /note="RLR CTR"
FT /evidence="ECO:0000259|PROSITE:PS51789"
SQ SEQUENCE 1002 AA; 113159 MW; 4431A6AAE8F68F18 CRC64;
MAADKDNTTN ISLIEDFRPR LRKLIEIEFV LDHLNFLDND NKDLIRTKAR KESNLKAVDL
LIDTIIRIRP LPKGWFREFV DALSAGGCKH AATYVEDSPP CPALEAENDN CVRLIELLSP
RLLGMKTTDV CWDCFSKGIL TAEDREIILA ECQNRGNMGG ARELPRRIVR FPPGWFSTFL
KALQVTEHND LCKELTGESP GDNLIDEPGV LMAVNEAPGN VYPMVEEGPV EAAGKSFLPE
QETLDSSVTN MHLDEPSEAD SEIADLYSGT EEPMENGKPE NSLDLSMSGC IAPAAESPPK
AVIVLRDYQM DVARPALEGK NIIICLPTGS GKTRVAVYIT KEHLDSRRKE GRPGKVVVLV
NKVPLVEQHY STEFWKFLKN KYKVERVSGD SQLKISFTDI VQKNDIVICT AQLLENYLER
AHSGDDDGIK LSDLSLIVID ECHHTQKGGV YNHIMIRYLK QKHKNAKLKK EQKDTVAIPQ
ILGLTASPGV GGAKKIEKAE EHILRICANL DAYKIMTGNL GENKKEPHKK IATAEERKED
PFGDVLKGVM NAIHIHAELN PTCDLGTQNY EQWVVQKEQN AAKEENQKVR VCAEHLRQYN
EALYLGKTIR MWDAFSFLDK YFDEELKKKV APGDEEAIIK TDTERFLFTL FKDSKVELQE
LAKLPQYENN SLAKLRTKIL HEFTSREKAR GIIFTKTRRS AIALAQWVQE NSKFEEVGVK
ACHVIGGGGQ SAVKPMTAAE QRDVLNKFQN AEINLLIATT VAEEGLDIAA CNFVIRYELV
TNEVAMIQAR GRGRAEDSSY TLVAGEGSGV AERESVNEYR EKMMSKAIDK VKKLDQEEYE
KRIKEFQIQA IMEERVRTTK KKQKGMKKES PSKVKFSCRS CNKPVCSGED VEIIENMHRV
NVTPQFKELF IQRENTSLVK RLLDYETNGF IACKDCGQRW GSMMLYRGIE CPCLHVKNFL
VTYDEKRKNV NKWSELGIQL PAFDYAEHAR SVAESSEDEE SM
//
