ID D7LY07_ARALL Unreviewed; 921 AA.
AC D7LY07;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 22-FEB-2023, entry version 52.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN ORFNames=ARALYDRAFT_327181 {ECO:0000313|EMBL:EFH48747.1};
OS Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=81972 {ECO:0000313|Proteomes:UP000008694};
RN [1] {ECO:0000313|Proteomes:UP000008694}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. MN47 {ECO:0000313|Proteomes:UP000008694};
RX PubMed=21478890; DOI=10.1038/ng.807;
RA Hu T.T., Pattyn P., Bakker E.G., Cao J., Cheng J.-F., Clark R.M.,
RA Fahlgren N., Fawcett J.A., Grimwood J., Gundlach H., Haberer G.,
RA Hollister J.D., Ossowski S., Ottilar R.P., Salamov A.A., Schneeberger K.,
RA Spannagl M., Wang X., Yang L., Nasrallah M.E., Bergelson J.,
RA Carrington J.C., Gaut B.S., Schmutz J., Mayer K.F.X., Van de Peer Y.,
RA Grigoriev I.V., Nordborg M., Weigel D., Guo Y.-L.;
RT "The Arabidopsis lyrata genome sequence and the basis of rapid genome size
RT change.";
RL Nat. Genet. 43:476-481(2011).
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR EMBL; GL348718; EFH48747.1; -; Genomic_DNA.
DR RefSeq; XP_002872488.1; XM_002872442.1.
DR AlphaFoldDB; D7LY07; -.
DR STRING; 81972.D7LY07; -.
DR EnsemblPlants; fgenesh1_pm.C_scaffold_6002401; fgenesh1_pm.C_scaffold_6002401; fgenesh1_pm.C_scaffold_6002401.
DR GeneID; 9310672; -.
DR Gramene; fgenesh1_pm.C_scaffold_6002401; fgenesh1_pm.C_scaffold_6002401; fgenesh1_pm.C_scaffold_6002401.
DR KEGG; aly:9310672; -.
DR eggNOG; KOG2119; Eukaryota.
DR HOGENOM; CLU_006322_1_0_1; -.
DR OrthoDB; 997839at2759; -.
DR Proteomes; UP000008694; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:EnsemblPlants.
DR GO; GO:0005886; C:plasma membrane; IEA:EnsemblPlants.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF29; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000008694}.
FT DOMAIN 86..403
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 493..853
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
SQ SEQUENCE 921 AA; 104488 MW; 044533B28E83184F CRC64;
MQKNDHMESE LQTQMVGNER LPQVTWQRKL NSKVKNPSEF KMSIRDVLHL FPIGYRLWSH
TKEEAKKGRA SMYDIFKKRH VTGDHGVPLG GIGGGSIGRS YKGEFQQFKL FPKVCEEAPI
LTNQFSVFVM RSGGVNYSSV LCPTKPKSVR GKTEELGIES WDWNMKGYKS MYHALYPRSW
TIYNEPDPEL RIVSRQISPF IPHNYKESSL PVSVFTFTVT NLGKEEATVT LLFTWENSVG
GASGLTGEHF NSTMMERDGV HAIALHHKTS NGHPPVTYAI AAQETEDVHV SECSCFLVSG
HSPKDITAKE MWDEIKKNKS FDGLNSEPGS PSRPGTSIGA AIAANVKVPP GCDRTVMFSL
SWDCPEVRFN EKTYHRRYTK FYGNLGDAAV KMARDALLNY VDWESQIEAW QSPILSDTAL
PDWYRVTLFN ELYYFNSGGT IWTDGLPPKE RIERSKIINT EQNNIVTELF KKINVICAQI
HSPQSLNAEE HIGQFIYLEG IEYLMYNTYD VHFYSSFALL SLFPKLELSI QRDFAATVLF
QDPTKKQIMS SGEWVPRKLL GSVPHDIGLN DPWLELNEYN FFNTDRWKDL NAKFVLQVYR
DVVSTGDQSF AKAVWPSVYT AVAYLDQFDK DEDEMIENEG FPDQTYDAWS VKGVSAYCGG
LWVAALQAAS AFASIVGENG VAIYFNTKYE KAKSVYEKLW NGSYFNYDDS GSGSSSSILA
DQLAGQWYAR ACGLKPITKE EWIKKALETI YEFNVMKVKG GTRGAVNGMS PDGQVDTNSL
VSKEVWAGTT YSVAACMIQE GQRERGFQTA SGIYEAVWSD RGLSCSFQTP EAWNMNDEYR
SLCYMRPLAI WSIQWALTRT QSFGEEKEKL VKGEEEESDL LLRQHKGFKD VARFVKIVPT
RNEHRSRLQH TYEAVLNILR L
//
