ID D7MFH1_ARALL Unreviewed; 943 AA.
AC D7MFH1;
DT 10-AUG-2010, integrated into UniProtKB/TrEMBL.
DT 10-AUG-2010, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=Small RNA 2'-O-methyltransferase {ECO:0000256|ARBA:ARBA00021330};
DE EC=2.1.1.386 {ECO:0000256|ARBA:ARBA00035025};
GN ORFNames=ARALYDRAFT_492795 {ECO:0000313|EMBL:EFH44128.1};
OS Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=81972 {ECO:0000313|Proteomes:UP000008694};
RN [1] {ECO:0000313|Proteomes:UP000008694}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. MN47 {ECO:0000313|Proteomes:UP000008694};
RX PubMed=21478890; DOI=10.1038/ng.807;
RA Hu T.T., Pattyn P., Bakker E.G., Cao J., Cheng J.-F., Clark R.M.,
RA Fahlgren N., Fawcett J.A., Grimwood J., Gundlach H., Haberer G.,
RA Hollister J.D., Ossowski S., Ottilar R.P., Salamov A.A., Schneeberger K.,
RA Spannagl M., Wang X., Yang L., Nasrallah M.E., Bergelson J.,
RA Carrington J.C., Gaut B.S., Schmutz J., Mayer K.F.X., Van de Peer Y.,
RA Grigoriev I.V., Nordborg M., Weigel D., Guo Y.-L.;
RT "The Arabidopsis lyrata genome sequence and the basis of rapid genome size
RT change.";
RL Nat. Genet. 43:476-481(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H(+) +
CC S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotide;
CC Xref=Rhea:RHEA:37887, Rhea:RHEA-COMP:10415, Rhea:RHEA-COMP:10416,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74896, ChEBI:CHEBI:74898; EC=2.1.1.386;
CC Evidence={ECO:0000256|ARBA:ARBA00034992};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. HEN1 family.
CC {ECO:0000256|ARBA:ARBA00009026}.
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DR EMBL; GL348719; EFH44128.1; -; Genomic_DNA.
DR RefSeq; XP_002867869.1; XM_002867823.1.
DR AlphaFoldDB; D7MFH1; -.
DR STRING; 81972.D7MFH1; -.
DR EnsemblPlants; fgenesh2_kg.7__2228__AT4G20910.1; fgenesh2_kg.7__2228__AT4G20910.1; fgenesh2_kg.7__2228__AT4G20910.1.
DR GeneID; 9303941; -.
DR Gramene; fgenesh2_kg.7__2228__AT4G20910.1; fgenesh2_kg.7__2228__AT4G20910.1; fgenesh2_kg.7__2228__AT4G20910.1.
DR KEGG; aly:9303941; -.
DR eggNOG; KOG1045; Eukaryota.
DR HOGENOM; CLU_001342_0_0_1; -.
DR OrthoDB; 2735228at2759; -.
DR Proteomes; UP000008694; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:EnsemblPlants.
DR GO; GO:0005634; C:nucleus; IEA:EnsemblPlants.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR GO; GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:EnsemblPlants.
DR GO; GO:0010589; P:leaf proximal/distal pattern formation; IEA:EnsemblPlants.
DR GO; GO:0010305; P:leaf vascular tissue pattern formation; IEA:EnsemblPlants.
DR GO; GO:0035196; P:miRNA processing; IEA:EnsemblPlants.
DR GO; GO:0035279; P:miRNA-mediated gene silencing by mRNA destabilization; IEA:EnsemblPlants.
DR GO; GO:0009909; P:regulation of flower development; IEA:EnsemblPlants.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR GO; GO:0009616; P:RNAi-mediated antiviral immune response; IEA:EnsemblPlants.
DR GO; GO:0010093; P:specification of floral organ identity; IEA:EnsemblPlants.
DR Gene3D; 3.10.50.40; -; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR026610; Hen1.
DR InterPro; IPR040870; HEN1_dsRBD2.
DR InterPro; IPR040813; Hen1_Lam_C.
DR InterPro; IPR006630; La_HTH.
DR InterPro; IPR025714; Methyltranfer_dom.
DR InterPro; IPR046357; PPIase_dom_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR21404; HEN1; 1.
DR PANTHER; PTHR21404:SF3; SMALL RNA 2'-O-METHYLTRANSFERASE; 1.
DR Pfam; PF17842; dsRBD2; 1.
DR Pfam; PF18441; Hen1_Lam_C; 1.
DR Pfam; PF21224; Hen1_LCD; 1.
DR Pfam; PF13847; Methyltransf_31; 1.
DR SUPFAM; SSF54534; FKBP-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS50961; HTH_LA; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000008694};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00332};
KW RNA-mediated gene silencing {ECO:0000256|ARBA:ARBA00023158};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 93..204
FT /note="HTH La-type RNA-binding"
FT /evidence="ECO:0000259|PROSITE:PS50961"
FT REGION 284..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 505..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 572..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 841..860
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 525..544
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 577..597
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 845..860
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 943 AA; 104241 MW; 4D9A16260FD12B86 CRC64;
MAGGGKHTPT PKAIIHQKFG AKASYRVEEV NDSSQSGCPG LAIPQKGPCL YRCHLQLPDF
SVVSNVFKKK KDSEQSAAEL ALEKLGIRPQ NDDLTVDEAW DEIVGRIKYI FSDEFLSAEH
PLGAHLRAAL RRDGERCGSV PVSVIATFDA KINSRCKIID PSVESDPFLA ISYVMKASAK
LSDYIVVSQH ALRRKNAYPS EIVEALATHV SDSLHRREVA AVYIPCIDEE VVELDTLYIS
SDRHYLDSIA ERLGLKDGSQ VMISSRTFGK ASCGSECRLY SDIPKKSSDN SSEASGSSNE
DSSQIKKSRN ARASYICGQD IHGDAILASV GYRWKSDDLN YDDVTVNSFY RICCGMSPNG
IYKISRQALI AAQLPFSFTT KSNWRGPLPR EILCLFCHQH RLAEPIISSS TAPVKSLSDI
FRSHKKLKVS GVDDANENLS REKEDTPGLG HGFRCEVKIF TKSQDLVLEC SPRKFYEKEN
DAIQNASLRA LLWFSKFFAD LDVDGEQPCD TDDDQDIKSP SPNVFAAPPI SQNEHSSESK
TTNVLSAEKH VQSITNGSVV SICYSLSLAV DSEDSSDGES PREDIESNED MESEGDAEYS
ANCEPSIDLI ESNEEIEFEV GTGSMNPHIE SAVTQMTVGE YSSFSITPPD AAEALILAVA
SDTVRIRSLL SERPSLNYSI LLLGVKGPSE ERMEAAYFKP PLSKQRVEYA LKHIRESSAS
TLVDFGCGSG SLLDSLLDYP TSLQTIIGVD ISPKGLARAA KMLHIKLNKE ACNVKSATLY
DGSILEFDSR LHDVDIGTCL EVIEHMEEDQ ACEFGEKVLS LFHPKLLIVS TPNYEFNTIL
QRSTSETQEE DKSGSQLPKF RNHDHKFEWT REQFNNWASN LAKRHNYGVE FSGVGGSGEV
EPGFASQIAV FRREASSVEN VAESSMQPYK VIWEWKKDGD KKD
//
