ID D7UTE0_9PSED Unreviewed; 375 AA.
AC D7UTE0;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=DNA gyrase subunit B {ECO:0000256|ARBA:ARBA00019166};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN Name=gyrB {ECO:0000313|EMBL:BAJ10899.1};
OS Pseudomonas flexibilis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=706570 {ECO:0000313|EMBL:BAJ10899.1};
RN [1] {ECO:0000313|EMBL:BAJ10899.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JCM 14085 {ECO:0000313|EMBL:BAJ10899.1};
RX PubMed=20817837; DOI=10.1099/ijs.0.024612-0;
RA Hirota K., Yamahira K., Nakajima K., Nodasaka Y., Okuyama H., Yumoto I.;
RT "Pseudomonas toyotomiensis sp. nov., a psychrotolerant facultative
RT alkaliphile that utilizes hydrocarbons.";
RL Int. J. Syst. Evol. Microbiol. 61:1842-1848(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
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DR EMBL; AB571150; BAJ10899.1; -; Genomic_DNA.
DR AlphaFoldDB; D7UTE0; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 302..375
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:BAJ10899.1"
FT NON_TER 375
FT /evidence="ECO:0000313|EMBL:BAJ10899.1"
SQ SEQUENCE 375 AA; 41221 MW; DA66C91AEA49C009 CRC64;
GVGVSVVNAL SEELVMTIRR AGKVWEQTYV HGVPQAPLAA VGETEGTGTQ IHFKPSAQTF
RNIHFSWDIL AKRLRELSFL NSGVGIVLRD ERSGKEELFK YEGGLKAFVE YLNTNKTAVN
SVFHFTVQRE EDGVGVEVAL QWNDGFNENI LCFTNNIPQR DGGTHLAGFR SALTRNLNNY
IEAEGLAKKF KISTTGDDAR EGLTAIISVK VPDPKFSSQT KDKLVSSEVK TAVEQEMGKF
FADFLLENPN DAKAVVGKMI DAARAREAAR KAREMTRRKG ALDIAGLPGK LADCQEKDPA
LSELYLVEGD SAGGSAKQGR NRRTQAILPL KGKILNVEKA RFDKMLSSQE VGTLITALGC
GIGRDEYNID KLRYH
//
