ID D8GQ32_CLOLD Unreviewed; 657 AA.
AC D8GQ32;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE SubName: Full=Enoate reductase {ECO:0000313|EMBL:ADK16123.1};
DE EC=1.3.1.31 {ECO:0000313|EMBL:ADK16123.1};
GN OrderedLocusNames=CLJU_c30750 {ECO:0000313|EMBL:ADK16123.1};
OS Clostridium ljungdahlii (strain ATCC 55383 / DSM 13528 / PETC).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=748727 {ECO:0000313|EMBL:ADK16123.1, ECO:0000313|Proteomes:UP000001656};
RN [1] {ECO:0000313|EMBL:ADK16123.1, ECO:0000313|Proteomes:UP000001656}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 55383 / DSM 13528 / PETC
RC {ECO:0000313|Proteomes:UP000001656};
RX PubMed=20616070; DOI=10.1073/pnas.1004716107;
RA Kopke M., Held C., Hujer S., Liesegang H., Wiezer A., Wollherr A.,
RA Ehrenreich A., Liebl W., Gottschalk G., Durre P.;
RT "Clostridium ljungdahlii represents a microbial production platform based
RT on syngas.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:13087-13092(2010).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: In the N-terminal section; belongs to the NADH:flavin
CC oxidoreductase/NADH oxidase family. {ECO:0000256|ARBA:ARBA00011048}.
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DR EMBL; CP001666; ADK16123.1; -; Genomic_DNA.
DR RefSeq; WP_013239712.1; NZ_LITS01000027.1.
DR AlphaFoldDB; D8GQ32; -.
DR STRING; 748727.CLJU_c30750; -.
DR KEGG; clj:CLJU_c30750; -.
DR PATRIC; fig|748727.19.peg.2240; -.
DR eggNOG; COG0446; Bacteria.
DR eggNOG; COG1902; Bacteria.
DR HOGENOM; CLU_012153_1_1_9; -.
DR OrthoDB; 9772736at2; -.
DR Proteomes; UP000001656; Chromosome.
DR GO; GO:0047540; F:2-enoate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR CDD; cd02803; OYE_like_FMN_family; 1.
DR CDD; cd01029; TOPRIM_primases; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR001155; OxRdtase_FMN_N.
DR InterPro; IPR034154; TOPRIM_DnaG/twinkle.
DR PANTHER; PTHR42917; 2,4-DIENOYL-COA REDUCTASE; 1.
DR PANTHER; PTHR42917:SF2; 2,4-DIENOYL-COA REDUCTASE [(2E)-ENOYL-COA-PRODUCING]; 1.
DR Pfam; PF00724; Oxidored_FMN; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00469; PNDRDTASEII.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023014};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00023014};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000313|EMBL:ADK16123.1}.
FT DOMAIN 4..343
FT /note="NADH:flavin oxidoreductase/NADH oxidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00724"
FT DOMAIN 396..628
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
SQ SEQUENCE 657 AA; 71573 MW; DBCFC60BEF82F937 CRC64;
MYQKLFSSKN IGSMTVRNRI VMTAMGNHLA EADGSVSESD IAFYGERAKG GVGLVITECA
CINLKIGKGN CHQIAVDDDK YIEGLKRMAD EIHKYGSVIA VQIYHPGRQG IAVINGNTSM
QAPSVTECQV VHQPTHEMTK EEIKDTIHRF IQGAKRLQKA GIDAVELHGA HGYMIGQFLS
PYTNKRTDEY GGSFENRMRF LDEIIAGVRK ECGSSYPIIV RYSADECMGY AGYPELGIHL
DEGVKIAKHL EAQGVDALDV SCGIYETMNT SWEPVGFDQG WKINIPAKIK ESVSIPVIGV
SVIREPEFAE KVLEEEKVDF IGSARQFFAD PEWGNKAQEG KEKSIRKCIS CLYCMETLMS
ADMGAAPMAC AINYQGGREN QYGDDCLKKD GKGRVVVVIG AGPSGMEAAR ILAKRKFKPI
VFEKNSKVGG QIVYASKPPK KEKTAWLIDY QKGQLDELGV EVKTNHAPTI DEIKALNPYA
VFVAQGSNPI MPKAIPGLDG EKVYTPVDIL SGKVTLKGKK VGVIGSGMTG IETAELLGSQ
GNKVTVFEMA DEIGPGIFFQ NLIDVMGRIA EHEIKLYPKH KLVKIDGTTV TVETTNTNET
KNFDFDAVVV SLGTASNKEL VEEIKTAFDK VVLLGDAAKA GRIEAAVGSG YKAAFEL
//
