ID D8K8V8_NITWC Unreviewed; 722 AA.
AC D8K8V8;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=Nwat_0196 {ECO:0000313|EMBL:ADJ27168.1};
OS Nitrosococcus watsoni (strain C-113).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales; Chromatiaceae;
OC Nitrosococcus.
OX NCBI_TaxID=105559 {ECO:0000313|EMBL:ADJ27168.1, ECO:0000313|Proteomes:UP000000393};
RN [1] {ECO:0000313|EMBL:ADJ27168.1, ECO:0000313|Proteomes:UP000000393}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C-113 {ECO:0000313|EMBL:ADJ27168.1,
RC ECO:0000313|Proteomes:UP000000393};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Malfatti S.A., Chain P.S.G., Land M., Hauser L., Kyrpides N.,
RA Ivanova N., Cambell M.A., Heidelberg J.F., Klotz M.G., Woyke T.;
RT "Complete sequence of chromosome of Nitrosococcus watsoni C-113.";
RL Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP002086; ADJ27168.1; -; Genomic_DNA.
DR RefSeq; WP_013219280.1; NC_014315.1.
DR AlphaFoldDB; D8K8V8; -.
DR STRING; 105559.Nwat_0196; -.
DR REBASE; 26858; M.NwaCORF196P.
DR KEGG; nwa:Nwat_0196; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_012122_0_0_6; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000000393; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ADJ27168.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 10..145
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 171..479
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 722 AA; 81063 MW; 83D6FD8D1BEAB4C8 CRC64;
MNHAIHNSIV NFIWGIADDV LRDVYVRGKY RDVILPMTVI RRLDALLEPT KEKVLVMKAQ
LDEAGIANQH AALCQAAGEA FYNVSPFTLR DLKSRAKLQQ LRADFEAYLD GFSPNVQEIL
DKFKFRNQIP TLIEADILGH LIDKFLDTRI NLSPRPVQDM DGNERLPALD NHAMGTIFEE
LIRRFNEENN EEAGEHFTPR DVVRLMADLI FLPIADEIES GTYLVYDGAC GTGGMLTVAE
ERLAELAASH GKEVSIHLFG QEVQPETYAI AKADLLLKGE GGGAENIKYG STLSSSDPFL
SQEFDFMLSN PPYGKSWKSD VDRLGGKDDI KDLRFVTHHG GDPAYKMITR SSDGQLMFLV
NNLAKMKPTT RLGSRIAEVH NGSSLFTGDA GQGESNIRRW IIENDWLEAI IALPENMFYN
TGIATYIWVL TNRKREKRRG KVQLIDASEW FVPLRRNLGK KNRELTEEHI RAICDLVVTP
VETEQSKIFP NEAFGYWKVT VDRPLRLAVD LSPARLERFE RTCAKSKEEP LANLARRVAG
VLGAGPHLDF NAFMDACGAD AKAHGIKLTA KRKKLLQSEL CDTREDAAPV LKKVHRPDKA
TPDPIHGLFK IELPSPRGRG AGGEGKIHVV EFEPDTALRD SEQVPLLEEG GIEAFFRREV
LPYTPDAWID PAKIQIGYEI SFTHHFYKPA PMRTLEAIKA DIYALEQETE GLLEQIVGEA
ER
//
