ID D8TIL0_VOLCA Unreviewed; 2481 AA.
AC D8TIL0;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=tRNA-uridine aminocarboxypropyltransferase 1 {ECO:0000256|ARBA:ARBA00039242};
DE EC=2.5.1.25 {ECO:0000256|ARBA:ARBA00012386};
DE AltName: Full=DTW domain-containing protein 1 {ECO:0000256|ARBA:ARBA00042508};
GN ORFNames=VOLCADRAFT_120236 {ECO:0000313|EMBL:EFJ53263.1};
OS Volvox carteri f. nagariensis.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Volvocaceae; Volvox.
OX NCBI_TaxID=3068 {ECO:0000313|Proteomes:UP000001058};
RN [1] {ECO:0000313|EMBL:EFJ53263.1, ECO:0000313|Proteomes:UP000001058}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=f. Nagariensis / Eve {ECO:0000313|Proteomes:UP000001058};
RX PubMed=20616280; DOI=10.1126/science.1188800;
RA Prochnik S.E., Umen J., Nedelcu A.M., Hallmann A., Miller S.M., Nishii I.,
RA Ferris P., Kuo A., Mitros T., Fritz-Laylin L.K., Hellsten U., Chapman J.,
RA Simakov O., Rensing S.A., Terry A., Pangilinan J., Kapitonov V., Jurka J.,
RA Salamov A., Shapiro H., Schmutz J., Grimwood J., Lindquist E., Lucas S.,
RA Grigoriev I.V., Schmitt R., Kirk D., Rokhsar D.S.;
RT "Genomic analysis of organismal complexity in the multicellular green alga
RT Volvox carteri.";
RL Science 329:223-226(2010).
CC -!- FUNCTION: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine
CC (acp3U) at position 20 in the D-loop of several cytoplasmic tRNAs
CC (acp3U(20)). {ECO:0000256|ARBA:ARBA00037050}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00024168};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the TDD superfamily. DTWD1 family.
CC {ECO:0000256|ARBA:ARBA00038290}.
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DR EMBL; GL378323; EFJ53263.1; -; Genomic_DNA.
DR RefSeq; XP_002946268.1; XM_002946222.1.
DR STRING; 3068.D8TIL0; -.
DR GeneID; 9621394; -.
DR KEGG; vcn:VOLCADRAFT_120236; -.
DR eggNOG; KOG3795; Eukaryota.
DR InParanoid; D8TIL0; -.
DR OrthoDB; 315417at2759; -.
DR Proteomes; UP000001058; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.730.10; AP2/ERF domain; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR InterPro; IPR005636; DTW.
DR PANTHER; PTHR15627; NATURAL KILLER CELL-SPECIFIC ANTIGEN KLIP1; 1.
DR PANTHER; PTHR15627:SF8; TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 1; 1.
DR Pfam; PF00847; AP2; 1.
DR Pfam; PF03942; DTW; 1.
DR SMART; SM00380; AP2; 1.
DR SMART; SM01144; DTW; 1.
DR SUPFAM; SSF54171; DNA-binding domain; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000001058};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 2038..2095
FT /note="AP2/ERF"
FT /evidence="ECO:0000259|PROSITE:PS51032"
FT REGION 247..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 653..679
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..765
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 937..988
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1056..1095
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1132..1157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1307..1360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1416..1437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1450..1476
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1520..1629
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1705..1729
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1809..2024
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2190..2288
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2332..2353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2418..2481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 247..329
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 367..384
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 484..533
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 733..748
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1335..1355
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1532..1548
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1852..1871
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2481 AA; 247552 MW; 98DEDE5E82DBB543 CRC64;
MAGLHSVPKL SARRPDWELP ELHGDLQLAP LDPLHNNTDR IKCPKCGRSR AHYCYDCLQP
LVPFPIVELP FRVSIVTHHE EKPSKNTGVQ VALLAPGQVD LHSMSQVPDD VDPSRCVVLF
PSDTALEVEQ LEPDSIDRLF IIDSRWKKAG ALMRTETFRS MCAVRLSHTR SAFWRFHTRG
VSDEGVCTVE ALYFFLEALV KHGKLSDPRF TAPHTFDNLL WYFVYQHQVV QQAALKRLQE
RGTAELLGQQ PQQQEGPGRA DTEQGQDQPH QQKPQQQQQR KGQTKQQQQQ QQKQKQNPMR
QTQKQNEQQQ QKKTQKQQQQ GQGQGQGDGP LTNLGGVGGP EVSGISEYGG ANTLAARKGP
AGNGGDGTTG GKQQQQQVLP SGDQPEESRR QLHAAEDAAV AVVAGPAGRE GKGEAGGGVV
REDEEIRCGP SGIGPAAAGV WEAGAPGAVH GDGSRNDGGD VDDAEGSGST TLQSGWADSR
VLPHPFASSN SSSLQVSLEL PPGQFTSPGT LPLHSPATSA PMTATSAVPQ PQPQPETLAA
VPGVRGVSCV IVDGQPSLGL SPPAAGGGRG ASASGAALPL LPQPPLGSGG VLQLAAPAAG
AAAATGPTAA ATATVQGSSA PAELTAPTCA SLPALCLPAV ASDVCGLAPV PSGASGGGGS
GGRVGIDSHH ATGQPAAAGG GAAVVAAGVA MPPQQQHPNP APPQHSGGLS LRRIKPVAAA
IDLHCALAAR KEQHPVPPPP PHPQPQQQPL LQSPSSAICD ASTSGRPGPY VPQCGLAGGS
SVLPIAPAVE GVPSAAAAGP AHSGSADGAP AASARAAVAV GLSAGVDGMK GMNAAHSTMQ
QLPPQQADGG SNETAGEGQV LWAPTAAVNT TADTAPWAGV VSSGHMGPYP GGRADAVGAS
GSGGGGTATT FTIGCNLLQQ GTGTTTALPC TYGSQARASG GGGGPDAGGG GGGGLVPTDC
VGPPAGAPIL SGRASDPGVS SFPQQQQQQQ VVLLPPGESA SASPAVKSSA HKAIAAIPVS
LQQQQQQQQL AGGSTSGGAI ADRFLASLVL PGVGGLTPPT EPASGGFSLP AEAAQEPPHP
QLQPQHLPQP VAAAAAAPQP QPLLFPPSLG IPPFSPAALQ LMAFSQMGQA VAATAEHQQH
HQALQSDQQQ QQQQQLAQLQ QHHQAAAALS LMQHMWQAPF GLVMPPGMMG MGMVPGMIWP
PGQMGLTPAA MPTPGGPLNP AAAAAAAAAA AAQFPALFNP AMMLPALAPF ALPLQAAATA
AAAQQLQQQQ QQQQQQPAAT VAAVNAAISG RSTAGNAGMT AAAAAVPPAL VKGSSRPTKS
AVKTEAAASV RGASATQEPA VQKQQQPQQP GGTTSCVGPE AAVRAPPTRC LTPLVPSALP
SAAAATTAAA AAAHPLVVQQ VRPDFTAFAG APAFSAAEAD SRPGPPPQTE ADDGGGVLAA
PAAANCRRGN TAAGAATRDG ASGDGNKDNS AVGAADADAE AEVEVAKKAP RSLPGLEVTR
LQSFASADLV VVDALQLLAA SPPPSPHRER RASGTSGASV TTAGGGGGSR RSSDGGGAAG
AAAGIGPTAT ADADTTTSGR PHTVPARRMF ELPDPVPVKR EPPQPQSGAA GLTSAAGQGP
RAAVTSAGEV RGPAATIPAS ARLPWLATSP ALPLPPRAAA AAAGTLDRQR LLHLAAVGAE
KVRRRASGQG GSGASLVGLE AAATGVADGR EDEPQLALPP GARRPKTRLA RAAAAPGGAF
LAVILEATEA AAAASAADAE IGGDGEEMET HAGAVATAQG LGLGHSRAPV VAGSQPLSAA
AVAAIGAGRS ARRSTGSGGG GGTRKVQWAT SEEAPFGSMR SAATAGAEED EGMVQGGEEE
DVGEEDGEEL EEVAAARGVR QRSRRWRHPA AAAAGSDGGG GGEGDEDDSD EDYNLGREAR
RRARGARRGG GGAGGATASV FAAARRAARG GGGGGSGGSA VDSDDGEGGL TSDDPAHGYT
GDGDDDTATA EGAAAAAAAA RRRRSGHGRP PGAGNLRKMK PRRLQSDGRL GFAMRSSRYT
GVYRTPGGRW RAQFCYRGAV HQLGMYDTER EAAAAWDQAV VTFRGSSSGV QLNLPQLLNS
YDLQNVREHI HRILDSKIAT TRTKRCLNSY ARNPPGPKSP SRVVPAAVIE NITTEPGTED
GGDVQLYDGG GDAAASGHVD LSGLPAAAAA AAAGGGGGGV GEVGSSQGRV RTATAAAGRA
CRRRRSPSPS GDGGPDDSGG DGASGSTAGG CRAARKRAHH DVGGGGGGGG AVLGNSSGGL
QPHTAAQDNT AGGQQLLLQA QPQRLGALPV GDAALLGLAA LRRHLPLPPP PSVAAGAAAD
TASLPPPPLR SHDLAGGDVT AAATTDIAAT AAAVATDAAV AAASAAAAVD DVLPPSKRSS
ETTTTADAAA AAAAAAASLV QPSTHEPWDV EGPAVSKVAA EAQHRSSSSG GGEGGDATGA
RSGRAASPKR RKSLAIRSRD Y
//
