ID D8V609_9VIBR Unreviewed; 453 AA.
AC D8V609;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 24-JAN-2024, entry version 48.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361153};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361153};
GN Name=cel5A {ECO:0000313|EMBL:ADJ93836.1};
GN ORFNames=SAMN04488136_104122 {ECO:0000313|EMBL:SDG89476.1};
OS Vibrio xiamenensis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=861298 {ECO:0000313|EMBL:ADJ93836.1};
RN [1] {ECO:0000313|EMBL:ADJ93836.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=G21 {ECO:0000313|EMBL:ADJ93836.1};
RX PubMed=20393708; DOI=10.1007/s00253-010-2554-y;
RA Gao Z., Ruan L., Chen X., Zhang Y., Xu X.;
RT "A novel salt-tolerant endo-beta-1,4-glucanase Cel5A in Vibrio sp. G21
RT isolated from mangrove soil.";
RL Appl. Microbiol. Biotechnol. 87:1373-1382(2010).
RN [2] {ECO:0000313|EMBL:SDG89476.1, ECO:0000313|Proteomes:UP000198854}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.10228 {ECO:0000313|EMBL:SDG89476.1,
RC ECO:0000313|Proteomes:UP000198854};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361153};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|RuleBase:RU361153}.
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DR EMBL; GQ397861; ADJ93836.1; -; Genomic_DNA.
DR EMBL; FNDD01000004; SDG89476.1; -; Genomic_DNA.
DR AlphaFoldDB; D8V609; -.
DR SMR; D8V609; -.
DR STRING; 861298.SAMN04488136_104122; -.
DR CAZy; CBM2; Carbohydrate-Binding Module Family 2.
DR CAZy; GH5; Glycoside Hydrolase Family 5.
DR OrthoDB; 9775889at2; -.
DR Proteomes; UP000198854; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030247; F:polysaccharide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.290; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001919; CBD2.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR34142:SF1; CELLULASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR34142; ENDO-BETA-1,4-GLUCANASE A; 1.
DR Pfam; PF00553; CBM_2; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SMART; SM00637; CBD_II; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR PROSITE; PS51173; CBM2; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361153};
KW Cellulose degradation {ECO:0000256|RuleBase:RU361153};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|RuleBase:RU361153};
KW Polysaccharide degradation {ECO:0000256|RuleBase:RU361153};
KW Reference proteome {ECO:0000313|Proteomes:UP000198854};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..453
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011937698"
FT DOMAIN 351..453
FT /note="CBM2"
FT /evidence="ECO:0000259|PROSITE:PS51173"
FT REGION 324..356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 453 AA; 47818 MW; 084419A306897CCA CRC64;
MTKQKCFQGK AVALAVALLS GSALASVPAL TVSGNQILSG GEATSFAGNS FFWSNTGWGQ
EKMYNASVVK WLKDDWGSSI VRVAIGADES GSYIDDPEAN LERAYKVIDA AIDNDMYVII
DFHTHHGEDK QAAAITFFQT MANRYGDNNN VIYEIYNEPL AVSWSDVIKP YAQNVIDAIR
AIDSDNLIVV GTPNWSQDVD VASQDPIEGS NIAYSLHFYA GTHGQYLRNK ASTALNNGVA
LMVTEWGAVN ADGDGDVASD SVDAWMDFLK TNNISNANWA VSDKDEGASI INSGASAEGN
WSASDLTDSG TYVKSIISNW GTSVDASSDE SSDTSSDSSD QASDSDTSTS DDSAASSSSV
TCEHVITSTW QGGFQGAIRI TNNGDSTVNG WNVNWSYSDG TTISQSWGAT LSGAYSATNL
SWNATIAPQQ TVEVGFIGSG DGSASAVTGD VCN
//
