ID D9T722_MICAI Unreviewed; 503 AA.
AC D9T722;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE SubName: Full=Alpha-N-arabinofuranosidase {ECO:0000313|EMBL:ADL46062.1};
DE EC=3.2.1.55 {ECO:0000313|EMBL:ADL46062.1};
GN OrderedLocusNames=Micau_2523 {ECO:0000313|EMBL:ADL46062.1};
OS Micromonospora aurantiaca (strain ATCC 27029 / DSM 43813 / BCRC 12538 / CBS
OS 129.76 / JCM 10878 / NBRC 16125 / NRRL B-16091 / INA 9442).
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=644283 {ECO:0000313|EMBL:ADL46062.1, ECO:0000313|Proteomes:UP000001908};
RN [1] {ECO:0000313|EMBL:ADL46062.1, ECO:0000313|Proteomes:UP000001908}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27029 / DSM 43813 / JCM 10878 / NBRC 16125 / INA 9442
RC {ECO:0000313|Proteomes:UP000001908};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Ovchinnikova G.,
RA Hirsch A.M., Woyke T.;
RT "Complete sequence of Micromonospora aurantiaca ATCC 27029.";
RL Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR EMBL; CP002162; ADL46062.1; -; Genomic_DNA.
DR RefSeq; WP_013285691.1; NC_014391.1.
DR AlphaFoldDB; D9T722; -.
DR STRING; 644283.Micau_2523; -.
DR CAZy; CBM42; Carbohydrate-Binding Module Family 42.
DR CAZy; GH43; Glycoside Hydrolase Family 43.
DR KEGG; mau:Micau_2523; -.
DR eggNOG; COG3940; Bacteria.
DR HOGENOM; CLU_009397_2_1_11; -.
DR OrthoDB; 177947at2; -.
DR Proteomes; UP000001908; Chromosome.
DR GO; GO:0046556; F:alpha-L-arabinofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046373; P:L-arabinose metabolic process; IEA:InterPro.
DR CDD; cd18817; GH43f_LbAraf43-like; 1.
DR Gene3D; 2.80.10.50; -; 1.
DR InterPro; IPR007934; AbfB_ABD.
DR InterPro; IPR036195; AbfB_ABD_sf.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43817; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR43817:SF1; HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED; 1.
DR Pfam; PF05270; AbfB; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF110221; AbfB domain; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187, ECO:0000313|EMBL:ADL46062.1};
KW Hydrolase {ECO:0000256|RuleBase:RU361187, ECO:0000313|EMBL:ADL46062.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001908}.
FT DOMAIN 373..501
FT /note="Alpha-L-arabinofuranosidase B arabinose-binding"
FT /evidence="ECO:0000259|Pfam:PF05270"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 69
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 246
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 184
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 503 AA; 55431 MW; 104610C6FBCD1E98 CRC64;
MSPRHLLHPA RRTAADPART ADPARRRLRP ILAAALAMLT ASAGLTAPGP AQAAPAVNYA
NPLVDQRADP HIVRHTDGYY YMTATVPQYD RIVLRRATTL QGLATATETT IWRRHTSGEM
GAHIWAPEIH FINGRWYVYF AAGRTDDVWR IRMYVLENAS ANPLTGSWTE RGRITTPWDT
FSLDASTFVV NGVRYLTWAQ QEPGIATNSN VYLARTGAEP WQITGPVARL VVPAYDWETR
GYRVAEGPAV IQRNGRVFLT YSASATDANY CLGMLTASAG ANLLDPASWS KSPTPVFASN
AATGQYGPGH NSFTVSEDGQ SDILVYHDRN YRDISGDPLN DPNRRTRIQK LYWNADGTPN
LGIPVPDGAT PVRLRSHDLP AAYVRHYDYR ARVEPNVTNL ADSQFRLVPG LAGAGSVSLE
STNFPGYYLR HRAFALYVER DDGSALFDGD ASFQRRPGLA TTEGLSLESQ NYPGRYVRHR
DGLLQLETVG TGADRAAATF HLE
//
