ID D9U513_9INFA Unreviewed; 744 AA.
AC D9U513;
DT 05-OCT-2010, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2010, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035, ECO:0000256|RuleBase:RU004330};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494, ECO:0000256|RuleBase:RU004330};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:ACN80302.1};
OS Influenza A virus (A/Taiwan/33/2007(H3N2)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=621193 {ECO:0000313|EMBL:ACN80302.1};
RN [1] {ECO:0000313|EMBL:ACN80302.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/Taiwan/33/2007 {ECO:0000313|EMBL:ACN80302.1};
RA Lin J.-H., Chiu S.-C., Liu H.-F.;
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ACN80302.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/Taiwan/33/2007 {ECO:0000313|EMBL:ACN80302.1};
RX PubMed=20370800; DOI=10.1111/j.1469-0691.2010.03228.x;
RA Lin J.H., Chiu S.C., Cheng J.C., Chang H.W., Hsiao K.L., Lin Y.C., Wu H.S.,
RA Liu H.F.;
RT "Molecular epidemiology and antigenic analyses of influenza A viruses H3N2
RT in Taiwan.";
RL Clin. Microbiol. Infect. 17:214-222(2011).
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|RuleBase:RU004330};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; FJ805738; ACN80302.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR Gene3D; 6.10.140.720; -; 1.
DR HAMAP; MF_04065; INFV_RDRP; 1.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PIRSF; PIRSF000827; RdRPol_OMV; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 3: Inferred from homology;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU004330};
KW Transferase {ECO:0000256|ARBA:ARBA00022484, ECO:0000256|RuleBase:RU004330};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT DOMAIN 273..470
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT REGION 37..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..52
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ACN80302.1"
FT NON_TER 744
FT /evidence="ECO:0000313|EMBL:ACN80302.1"
SQ SEQUENCE 744 AA; 84899 MW; A61F23727AFBDA97 CRC64;
AQNAISTTFP YTGDPPYSHG TGTGYTMDTV NRTHQYSEKG KWTTNTETGA PQLNPIDGPL
PEDNEPSGYA QTDCVLEAMA FLEESHPGIF ENSCLETMEA VQQTRVDKLT QGRQTYDWTL
NRNQPAATAL ANTIEVFRSN GLTANESGRL IDFLKDVMES MDKEEMEITT HFQRKRRVRD
NMTKKMVTQR TIGKKKQRVN KRGYLIRALT LNTMTKDAER GKLKRRAIAT PGMQIRGFVY
FVETLARSIC EKLEQSGLPV GGNEKKAKLA NVVRKMMTNS QDTELSFTIT GDNTKWNENQ
NPRMFLAMIT YITKNQPEWF RNILSIAPIM FSNKMARLGK GYMFESKRMK LRTQIPAEML
ASIDLKYFNE STRKKIEKIR PLLIDGTASL SPGMMMGMFN MLSTVLGVSI LNLGQKKYTK
TTYWWDGLQS SDDFALIVNA PNHEGIQAGV DRFYRTCKLV GINMSKKKSY INKTGTFEFT
SFFYRYGFVA NFSMELPSFG VSGINESADM SIGVTVIKNN MINNDLGPAT AQMALQLFIK
DYRYTYRCHR GDTQIQTRRS FELKKLWDQT QSKAGLLVSD GGPNLYNIRN LHIPEVCLKW
ELMDENYRGR LCNPLNPFVS HKEIESVNNA VVMPAHGPAK SMEYDAVATT HSWIPKRNRS
ILNTSQRGIL EDEQMYQKCC NLFEKFFPSS SYRRPIGISS MVEAMVSRAR IDARIDFESG
RIKKEEFSEI MKICSTIEEL RRQK
//
