ID DIT21_ARATH Reviewed; 563 AA.
AC Q9FMF7;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 123.
DE RecName: Full=Dicarboxylate transporter 2.1, chloroplastic;
DE AltName: Full=AtpDCT1;
DE AltName: Full=Glutamate/malate translocator;
DE Flags: Precursor;
GN Name=DIT2-1; Synonyms=DIT2; OrderedLocusNames=At5g64290; ORFNames=MSJ1.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=12154133; DOI=10.1093/pcp/pcf109;
RA Taniguchi M., Taniguchi Y., Kawasaki M., Takeda S., Kato T., Sato S.,
RA Tabata S., Miyake H., Sugiyama T.;
RT "Identifying and characterizing plastidic 2-oxoglutarate/malate and
RT dicarboxylate transporters in Arabidopsis thaliana.";
RL Plant Cell Physiol. 43:706-717(2002).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF GLY-206.
RX PubMed=12887583; DOI=10.1046/j.1365-313x.2003.01806.x;
RA Renne P., Dressen U., Hebbeker U., Hille D., Flugge U.I., Westhoff P.,
RA Weber A.P.;
RT "The Arabidopsis mutant dct is deficient in the plastidic glutamate/malate
RT translocator DiT2.";
RL Plant J. 35:316-331(2003).
CC -!- FUNCTION: Glutamate/malate translocator involved with DIT1 in primary
CC ammonia assimilation and in the re-assimilation of ammonia generated by
CC the photorespiratory pathway. Exports the end product of ammonia
CC assimilation, glutamate, from plastids to the cytosol. The precursor
CC for ammonia assimilation, 2-oxoglutarate, is imported from the cytosol
CC by DIT1. {ECO:0000269|PubMed:12154133, ECO:0000269|PubMed:12887583}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.41 mM for malate {ECO:0000269|PubMed:12154133};
CC KM=0.23 mM for oxaloacetate {ECO:0000269|PubMed:12154133};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, rosette and cauline leaves,
CC stems, flowers and siliques. {ECO:0000269|PubMed:12154133,
CC ECO:0000269|PubMed:12887583}.
CC -!- DISRUPTION PHENOTYPE: Non-viable seedlings under normal atmospheric
CC conditions, but grow normally under non-photorespiratory conditions
CC high CO(2) conditions. {ECO:0000269|PubMed:12154133}.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC DIT1 subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB008268; BAB09860.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97866.1; -; Genomic_DNA.
DR EMBL; AY056329; AAL07178.1; -; mRNA.
DR EMBL; BT000654; AAN31801.1; -; mRNA.
DR EMBL; BT015925; AAU95461.1; -; mRNA.
DR RefSeq; NP_201234.1; NM_125825.3.
DR AlphaFoldDB; Q9FMF7; -.
DR SMR; Q9FMF7; -.
DR BioGRID; 21792; 2.
DR IntAct; Q9FMF7; 1.
DR STRING; 3702.Q9FMF7; -.
DR SwissPalm; Q9FMF7; -.
DR PaxDb; 3702-AT5G64290-1; -.
DR ProteomicsDB; 222150; -.
DR EnsemblPlants; AT5G64290.1; AT5G64290.1; AT5G64290.
DR GeneID; 836550; -.
DR Gramene; AT5G64290.1; AT5G64290.1; AT5G64290.
DR KEGG; ath:AT5G64290; -.
DR Araport; AT5G64290; -.
DR TAIR; AT5G64290; DIT2.1.
DR eggNOG; ENOG502QQ8W; Eukaryota.
DR HOGENOM; CLU_005170_7_3_1; -.
DR InParanoid; Q9FMF7; -.
DR OMA; PQAWRYF; -.
DR OrthoDB; 1330510at2759; -.
DR PhylomeDB; Q9FMF7; -.
DR SABIO-RK; Q9FMF7; -.
DR PRO; PR:Q9FMF7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMF7; baseline and differential.
DR Genevisible; Q9FMF7; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0015140; F:malate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0015131; F:oxaloacetate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0019676; P:ammonia assimilation cycle; IMP:UniProtKB.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; IDA:UniProtKB.
DR GO; GO:0071423; P:malate transmembrane transport; IDA:UniProtKB.
DR GO; GO:0015729; P:oxaloacetate transport; IDA:UniProtKB.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR InterPro; IPR030676; CitT-rel.
DR InterPro; IPR001898; SLC13A/DASS.
DR NCBIfam; TIGR00785; dass; 1.
DR PANTHER; PTHR42826; DICARBOXYLATE TRANSPORTER 2.1, CHLOROPLASTIC; 1.
DR PANTHER; PTHR42826:SF1; DICARBOXYLATE TRANSPORTER 2.1, CHLOROPLASTIC; 1.
DR Pfam; PF00939; Na_sulph_symp; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Plastid inner membrane; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..68
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 69..563
FT /note="Dicarboxylate transporter 2.1, chloroplastic"
FT /id="PRO_0000419184"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 537..557
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 16..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 71..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..92
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 206
FT /note="G->E: In dct; photorespiratory phenotype leading to
FT non-viable seedlings under normal atmospheric conditions."
FT /evidence="ECO:0000269|PubMed:12887583"
SQ SEQUENCE 563 AA; 59992 MW; BAE5C455D5FF88A0 CRC64;
MESFALHSLS TTATSTLLSH HHHHHPSRLS LLRRTSSRSP PSTISLRSLS VQPLSFPLLK
PIPRFSTRIA AAPQDNAPPP PPPSPSPSPS PQGAKLIPLI LSISVGLILR FAVPVPEGVT
PQGWQLLSIF LSTIAGLVLS PLPVGAWAFI GLTASIVTKT LSFSAAFSAF TSEVIWLIVI
SFFFARGFVK TGLGDRIATY FVKWLGKSTL GLSYGLTLSE ALIAPAMPST TARAGGIFLP
IIKSLSLSAG SKPNDSSSRK LGSYLIQSQF QCAGNSSALF LTAAAQNLLC LKLAEELGVV
ISNPWVSWFK AASLPAIISL LCTPLILYKL YPPETKDTPE APGIAATKLK QMGPVTKNEW
IMVGTMLLAV TLWICGETLG IPSVVAAMIG LSILLVLGVL NWDDCLSEKS AWDTLAWFAV
LVGMAGQLTN LGVVTWMSDC VAKVLQSLSL SWPAAFGLLQ AAYFFIHYLF ASQTGHVGAL
FSAFLAMHIA AGVPGILAAL ALAYNTNLFG ALTHYSSGQA AVYYGAGYVD LPDVFKIGFV
MATINAIIWG VVGTFWWKFL GLY
//
