GenomeNet

Database: UniProt
Entry: DNAA_BACSU
LinkDB: DNAA_BACSU
Original site: DNAA_BACSU 
ID   DNAA_BACSU              Reviewed;         446 AA.
AC   P05648;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   07-JUN-2017, entry version 138.
DE   RecName: Full=Chromosomal replication initiator protein DnaA;
GN   Name=dnaA; Synonyms=dnaH; OrderedLocusNames=BSU00010;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987847; DOI=10.1093/nar/13.7.2251;
RA   Moriya S., Ogasawara N., Yoshikawa H.;
RT   "Structure and function of the region of the replication origin of the
RT   Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000
RT   base pairs in the origin region.";
RL   Nucleic Acids Res. 13:2251-2265(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA   Ogasawara N., Nakai S., Yoshikawa H.;
RT   "Systematic sequencing of the 180 kilobase region of the Bacillus
RT   subtilis chromosome containing the replication origin.";
RL   DNA Res. 1:1-14(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6 AND 441-446.
RC   STRAIN=168 / PSL1;
RX   PubMed=2846289;
RA   Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H.;
RT   "Regulation of initiation of the chromosomal replication by DnaA-boxes
RT   in the origin region of the Bacillus subtilis chromosome.";
RL   EMBO J. 7:2911-2917(1988).
CC   -!- FUNCTION: Plays an important role in the initiation and regulation
CC       of chromosomal replication. Binds to the origin of replication; it
CC       binds specifically double-stranded DNA at a 9 bp consensus (dnaA
CC       box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic
CC       phospholipids. DnaA can inhibit its own gene expression as well as
CC       that of other genes.
CC   -!- SUBUNIT: Some 20 DnaA protein molecules bind their sites in oriC.
CC   -!- INTERACTION:
CC       P37522:soj; NbExp=2; IntAct=EBI-2014722, EBI-2014674;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DnaA family. {ECO:0000305}.
DR   EMBL; X02369; CAA26217.1; -; Genomic_DNA.
DR   EMBL; D26185; BAA05237.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB11777.1; -; Genomic_DNA.
DR   EMBL; X12778; CAA31269.1; -; Genomic_DNA.
DR   EMBL; X12779; CAA31270.1; -; Genomic_DNA.
DR   PIR; A22930; IQBSOC.
DR   RefSeq; NP_387882.1; NC_000964.3.
DR   RefSeq; WP_003242674.1; NZ_JNCM01000034.1.
DR   PDB; 4TPS; X-ray; 1.65 A; B/D=1-82.
DR   PDBsum; 4TPS; -.
DR   ProteinModelPortal; P05648; -.
DR   SMR; P05648; -.
DR   DIP; DIP-41940N; -.
DR   IntAct; P05648; 7.
DR   MINT; MINT-188900; -.
DR   STRING; 224308.Bsubs1_010100000005; -.
DR   PaxDb; P05648; -.
DR   EnsemblBacteria; CAB11777; CAB11777; BSU00010.
DR   GeneID; 939978; -.
DR   KEGG; bsu:BSU00010; -.
DR   PATRIC; fig|224308.179.peg.1; -.
DR   eggNOG; ENOG4105CI4; Bacteria.
DR   eggNOG; COG0593; LUCA.
DR   HOGENOM; HOG000235658; -.
DR   InParanoid; P05648; -.
DR   KO; K02313; -.
DR   OMA; REFNPLF; -.
DR   PhylomeDB; P05648; -.
DR   BioCyc; BSUB:BSU00010-MONOMER; -.
DR   PRO; PR:P05648; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR   GO; GO:0006270; P:DNA replication initiation; IDA:CACAO.
DR   GO; GO:0006275; P:regulation of DNA replication; IEA:InterPro.
DR   CDD; cd06571; Bac_DnaA_C; 1.
DR   Gene3D; 1.10.1750.10; -; 1.
DR   HAMAP; MF_00377; DnaA_bact; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR001957; Chromosome_initiator_DnaA.
DR   InterPro; IPR020591; Chromosome_initiator_DnaA-like.
DR   InterPro; IPR018312; Chromosome_initiator_DnaA_CS.
DR   InterPro; IPR013317; DnaA.
DR   InterPro; IPR013159; DnaA_C.
DR   InterPro; IPR024633; DnaA_N_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR010921; Trp_repressor/repl_initiator.
DR   PANTHER; PTHR30050:SF12; PTHR30050:SF12; 1.
DR   Pfam; PF00308; Bac_DnaA; 1.
DR   Pfam; PF08299; Bac_DnaA_C; 1.
DR   Pfam; PF11638; DnaA_N; 1.
DR   PRINTS; PR00051; DNAA.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00760; Bac_DnaA_C; 1.
DR   SUPFAM; SSF48295; SSF48295; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00362; DnaA; 1.
DR   PROSITE; PS01008; DNAA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Complete proteome; Cytoplasm;
KW   DNA replication; DNA-binding; Nucleotide-binding; Reference proteome.
FT   CHAIN         1    446       Chromosomal replication initiator protein
FT                                DnaA.
FT                                /FTId=PRO_0000114133.
FT   NP_BIND     151    158       ATP. {ECO:0000255}.
FT   HELIX         1     16       {ECO:0000244|PDB:4TPS}.
FT   HELIX        21     27       {ECO:0000244|PDB:4TPS}.
FT   TURN         28     30       {ECO:0000244|PDB:4TPS}.
FT   STRAND       32     37       {ECO:0000244|PDB:4TPS}.
FT   STRAND       40     44       {ECO:0000244|PDB:4TPS}.
FT   HELIX        48     57       {ECO:0000244|PDB:4TPS}.
FT   HELIX        59     70       {ECO:0000244|PDB:4TPS}.
FT   STRAND       75     79       {ECO:0000244|PDB:4TPS}.
SQ   SEQUENCE   446 AA;  50859 MW;  666799A0A183C992 CRC64;
     MENILDLWNQ ALAQIEKKLS KPSFETWMKS TKAHSLQGDT LTITAPNEFA RDWLESRYLH
     LIADTIYELT GEELSIKFVI PQNQDVEDFM PKPQVKKAVK EDTSDFPQNM LNPKYTFDTF
     VIGSGNRFAH AASLAVAEAP AKAYNPLFIY GGVGLGKTHL MHAIGHYVID HNPSAKVVYL
     SSEKFTNEFI NSIRDNKAVD FRNRYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNTLHEES
     KQIVISSDRP PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV
     MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPSSK PKVITIKEIQ
     RVVGQQFNIK LEDFKAKKRT KSVAFPRQIA MYLSREMTDS SLPKIGEEFG GRDHTTVIHA
     HEKISKLLAD DEQLQQHVKE IKEQLK
//
DBGET integrated database retrieval system