ID DUT_HHV11 Reviewed; 371 AA.
AC P10234; Q09I84;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 24-JAN-2024, entry version 92.
DE RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase {ECO:0000255|HAMAP-Rule:MF_04031};
DE Short=dUTPase {ECO:0000255|HAMAP-Rule:MF_04031};
DE EC=3.6.1.23 {ECO:0000255|HAMAP-Rule:MF_04031};
DE AltName: Full=dUTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_04031};
GN Name=DUT {ECO:0000255|HAMAP-Rule:MF_04031}; OrderedLocusNames=UL50;
OS Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Orthoherpesviridae; Alphaherpesvirinae; Simplexvirus;
OC Simplexvirus humanalpha1; Human herpesvirus 1.
OX NCBI_TaxID=10299;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=2839594; DOI=10.1099/0022-1317-69-7-1531;
RA McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D.,
RA Perry L.J., Scott J.E., Taylor P.;
RT "The complete DNA sequence of the long unique region in the genome of
RT herpes simplex virus type 1.";
RL J. Gen. Virol. 69:1531-1574(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nonneuroinvasive mutant HF10;
RX PubMed=17218138; DOI=10.1016/j.micinf.2006.10.019;
RA Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.;
RT "Determination and analysis of the DNA sequence of highly attenuated herpes
RT simplex virus type 1 mutant HF10, a potential oncolytic virus.";
RL Microbes Infect. 9:142-149(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17 syn+;
RA Cunningham C., Davison A.J.;
RT "Herpes simplex virus type 1 bacterial artificial chromosome.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in nucleotide metabolism: produces dUMP, the
CC immediate precursor of thymidine nucleotides and decreases the
CC intracellular concentration of dUTP to avoid uracil incorporation into
CC viral DNA. {ECO:0000255|HAMAP-Rule:MF_04031}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_04031};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_04031};
CC -!- SIMILARITY: Belongs to the dUTPase family. {ECO:0000255|HAMAP-
CC Rule:MF_04031}.
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DR EMBL; X14112; CAA32301.1; -; Genomic_DNA.
DR EMBL; DQ889502; ABI63511.1; -; Genomic_DNA.
DR EMBL; FJ593289; ACM62274.1; -; Genomic_DNA.
DR PIR; E30089; WMBEY0.
DR RefSeq; YP_009137126.1; NC_001806.2.
DR BioGRID; 971447; 1.
DR DIP; DIP-62052N; -.
DR IntAct; P10234; 1.
DR DNASU; 2703421; -.
DR GeneID; 2703421; -.
DR KEGG; vg:2703421; -.
DR Proteomes; UP000009294; Genome.
DR Proteomes; UP000180652; Genome.
DR GO; GO:0004170; F:dUTP diphosphatase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046080; P:dUTP metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 2.70.40.10; -; 2.
DR HAMAP; MF_04031; HSV_DUT; 1.
DR InterPro; IPR029054; dUTPase-like.
DR InterPro; IPR036157; dUTPase-like_sf.
DR InterPro; IPR034745; HSV_DUT.
DR Pfam; PF00692; dUTPase; 1.
DR SUPFAM; SSF51283; dUTPase-like; 2.
PE 3: Inferred from homology;
KW Hydrolase; Magnesium; Metal-binding; Nucleotide metabolism;
KW Reference proteome.
FT CHAIN 1..371
FT /note="Deoxyuridine 5'-triphosphate nucleotidohydrolase"
FT /id="PRO_0000182952"
FT REGION 350..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 260..262
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04031"
FT BINDING 366..367
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04031"
SQ SEQUENCE 371 AA; 39128 MW; 5FFA2556AD2F7158 CRC64;
MSQWGSGAIL VQPDSLGRGY DGDWHTAVAT RGGGVVQLNL VNRRAVAFMP KVSGDSGWAV
GRVSLDLRMA MPADFCAIIH APALASPGHH VILGLIDSGY RGTVMAVVVA PKRTREFAPG
TLRVDVTFLD ILATPPALTE PISLRQFPQL APPPPTGAGI REDPWLEGAL GAPSVTTALP
ARRRGRSLVY AGELTPVQTE HGDGVREAIA FLPKREEDAG FDIVVRRPVT VPANGTTVVQ
PSLRMLHADA GPAACYVLGR SSLNARGLLV VPTRWLPGHV CAFVVYNLTG VPVTLEAGAK
VAQLLVAGAD ALPWIPPDNF HGTKALRNYP RGVPDSTAEP RNPPLLVFTN EFDAEAPPSE
RGTGGFGSTG I
//
