ID E1NQT5_9LACO Unreviewed; 629 AA.
AC E1NQT5;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=HMPREF9212_1035 {ECO:0000313|EMBL:EFO68548.1};
OS Lactobacillus iners LactinV 03V1-b.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=879298 {ECO:0000313|EMBL:EFO68548.1, ECO:0000313|Proteomes:UP000005160};
RN [1] {ECO:0000313|EMBL:EFO68548.1, ECO:0000313|Proteomes:UP000005160}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LactinV 03V1-b {ECO:0000313|EMBL:EFO68548.1,
RC ECO:0000313|Proteomes:UP000005160};
RA Durkin A.S., Madupu R., Torralba M., Gillis M., Methe B., Sutton G.,
RA Nelson K.E.;
RL Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFO68548.1}.
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DR EMBL; AEHP01000066; EFO68548.1; -; Genomic_DNA.
DR AlphaFoldDB; E1NQT5; -.
DR Proteomes; UP000005160; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR NCBIfam; TIGR02074; PBP_1a_fam; 1.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF29; PENICILLIN-BINDING PROTEIN 1A; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 26..53
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 78..255
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 349..626
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
SQ SEQUENCE 629 AA; 69928 MW; 6DAB0EB433BEDB11 CRC64;
MSESKNSSER NTRSSKYHKN KKEKHLGWLV LKLILLFVVV IIVSGVGLFA YYAKDAPSIS
KAQLQSGGTS SLYTTDGKFL LSLGSEKRIY VDDKHIPQKL KDAVISVEDK RFYSEGFGLD
PVRIVGSVLV NARAKGVAAG GSTITQQLVK LSVFSTAMSQ RTLKRKAQEA WLSMKVEREF
SKKQILEFYI NKVYMNYGNY GMGTAADFYY GKPLKDLNLA QTALIAGMPN APISYDPYVY
PKKAKYRRDI VLYSMLKNKK ISSAQYNAAV KVPITDGLLS KKDKVNSNLR KVDDPYIKEV
INEVKEKGFN PYNDNLKITI NINQHIQQKL YDLANNGEVP FTNDKMQIGA TIIDPNNGHV
VAILGGRKLP AVQLGLNRAV QTARSTGSTI KPVLDYAPAI EYLKWSTSHI INDSKYIYPG
TKIQLYDWDN VYQGKMSIRR ALEQSRNVPA VKTLKKVGLA RASLFVRKMG INIPSDSGLS
VGIGANASTL QMASAYAAFA NNGRYYKPQF VSKIETPDGI TRNYDSSSTK VMKESTAYMI
TDMLKGVLTR GSGTMAKCDN IYEAGKTGTV KYSDEDLVKF PSYAHTPKDS WFIGYTKAYS
IGIWTGYDNL KDGTISGIGE NSAQLFYKK
//
