ID E1Z526_CHLVA Unreviewed; 922 AA.
AC E1Z526;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=CHLNCDRAFT_56762 {ECO:0000313|EMBL:EFN59449.1};
OS Chlorella variabilis (Green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Chlorella clade; Chlorella.
OX NCBI_TaxID=554065 {ECO:0000313|Proteomes:UP000008141};
RN [1] {ECO:0000313|EMBL:EFN59449.1, ECO:0000313|Proteomes:UP000008141}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NC64A {ECO:0000313|EMBL:EFN59449.1,
RC ECO:0000313|Proteomes:UP000008141};
RX PubMed=20852019; DOI=10.1105/tpc.110.076406;
RA Blanc G., Duncan G., Agarkova I., Borodovsky M., Gurnon J., Kuo A.,
RA Lindquist E., Lucas S., Pangilinan J., Polle J., Salamov A., Terry A.,
RA Yamada T., Dunigan D.D., Grigoriev I.V., Claverie J.M., Van Etten J.L.;
RT "The Chlorella variabilis NC64A genome reveals adaptation to
RT photosymbiosis, coevolution with viruses, and cryptic sex.";
RL Plant Cell 22:2943-2955(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00560}.
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DR EMBL; GL433836; EFN59449.1; -; Genomic_DNA.
DR RefSeq; XP_005851551.1; XM_005851489.1.
DR AlphaFoldDB; E1Z526; -.
DR STRING; 554065.E1Z526; -.
DR GeneID; 17358418; -.
DR KEGG; cvr:CHLNCDRAFT_56762; -.
DR eggNOG; ENOG502SVD3; Eukaryota.
DR InParanoid; E1Z526; -.
DR OrthoDB; 208346at2759; -.
DR Proteomes; UP000008141; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.10.160; -; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000008141}.
FT DOMAIN 1..403
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 328..715
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 619..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 780..809
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 922 AA; 99176 MW; BDB95FC997191CDF CRC64;
MTGRARFLLG QGVAAEEVLV LTLSRKAAGE FRERLLRHVP GAKAATVCTF HSWSWMVVWR
HWREAGFERR PTILAAEQQE LALMRDCIMW DQLAAAQAEM RGWLFLPPSV PWPQVVAAVA
ERHAPLFHKC MQAALAAHSS EQQPGSRPAP SAGEAAARSF AELPTRLQLL LVGELHASLQ
QRFDGHCRGE PDGGVLELLA AADAQALFFL RWLEGEKRRG HMPDGAAYAA LCARVGEGQE
RQRQLARVAV AYQAELRRQC LVPLSDLPLL ARQLLAARGG ALAWAQERWT HLLVDEFQDS
AACMIFADYQ RHFGARKLCL QTNFRSTAAI CAFGCTLLHG NRRDAEGGMV PARAGACRPV
HVRQFHTAEE EAAALADEVL RLWREEGVAY SSVCVLYRCL RLQGAELHAP LTASLRRLGI
PHVIAGHGRL AEDPAVAALL AYLAASLEVD PAFEVVLGLP HHGLGREVCG ALAQQQAAAR
LAGQLPPSLL ACAEQVCTQA EAMLTQAPWQ GQQAAVQVGP QSASQLEHVV SQLYTGDLQE
LSRLVLLLRG MRRAVGQEPL PRALRRVLQD SGLAAWLQQH GPAGAAAAVG SQQQRLGGGG
DLPASLRCVL DKAEQLAAEW QQQQAEGRGR RPAGSGVGGA ADGGSWCPAH AAGAASQQEA
GEELVRELLA RLATDTAADE CAVQRRSGAA SQRQQQAPGC DPGALTIGTI HSAKGLEWPV
VLLPSVCQGL LPLRFRPPAW QAGDGAAALR ACPRAHLEEE RRLFHVAATR ARDTLLVSFV
QPPPPGAPAE PGDEELEASA APAPQPELEP AGELRCSSIL AGTVHRLRHQ PDVLRFEGRL
PHQDCCQAEW EEEEAQAGGM LHEVGNRPGS HGGPCQPQAV KLSLAERARR QLADNPRAGK
LRARGGVAKQ AQRQCPAAVL QG
//
