ID E2N753_9BACE Unreviewed; 1229 AA.
AC E2N753;
DT 30-NOV-2010, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
GN ORFNames=BACCELL_00095 {ECO:0000313|EMBL:EEF92273.1};
OS Bacteroides cellulosilyticus DSM 14838.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=537012 {ECO:0000313|EMBL:EEF92273.1, ECO:0000313|Proteomes:UP000003711};
RN [1] {ECO:0000313|EMBL:EEF92273.1, ECO:0000313|Proteomes:UP000003711}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14838 {ECO:0000313|EMBL:EEF92273.1,
RC ECO:0000313|Proteomes:UP000003711};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEF92273.1, ECO:0000313|Proteomes:UP000003711}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14838 {ECO:0000313|EMBL:EEF92273.1,
RC ECO:0000313|Proteomes:UP000003711};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Bacteroides cellulosilyticus (DSM 14838).";
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEF92273.1}.
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DR EMBL; ACCH01000006; EEF92273.1; -; Genomic_DNA.
DR RefSeq; WP_007209492.1; NZ_JANSWE010000051.1.
DR AlphaFoldDB; E2N753; -.
DR HOGENOM; CLU_270292_0_0_10; -.
DR Proteomes; UP000003711; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:EEF92273.1};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..1229
FT /note="beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003161312"
FT DOMAIN 246..410
FT /note="Glycosyl hydrolases family 2 sugar binding"
FT /evidence="ECO:0000259|Pfam:PF02837"
FT DOMAIN 466..573
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 576..807
FT /note="Glycoside hydrolase family 2 catalytic"
FT /evidence="ECO:0000259|Pfam:PF02836"
SQ SEQUENCE 1229 AA; 140252 MW; 9F1081A6AF645B86 CRC64;
MRKLYATFLI SACALVSLPS NAQAEWKNDF SNQGEILKTV GKGVCGVTDG VFRSVDAYSC
FGNPEWKNYT MSFKARAPKD ADQVQIWAGF RAYNRFDRYV LGLKGGLQDD IYLMRLGYMG
TDEFLGVRPL GFHPVPGEWY NIKVEVCGNR IRVFLNNEKE PRIDVTDKNG NLAPSGQVTL
GGGWIETEFD DLVVTPMRED ALKDVKVVEY RKMITPQEKE NKRQQERANY RTVKVNELVD
SRTDISLDGT WLFMPEYQLD DKDKAISVAT DDKNWHVMSV PNFWNPIRIW LHGETMPSPA
GPQPKGVSDT YYQQETERCE GYTFDYRKTK AAWYRQWVEL PANVEGKNMT LTFDAVSKVA
EIYINGELAG SHIGMFGEIQ VDGSKLLKSG KNLVVVKVIS KTDGSSSESG SAAIDFFYSS
VRESEKEDGK VSAKSNLLKD IAHGFYGDEP AGIWQPVKLT ITSPLKVENV FIKPSLDGAT
FDLTVKNHGT KKNQFNLYTD IVDKETGSVL YSKLSLQKLV LNADEEKMFT YNINGLKPRL
WTPQHPNLYD FRFRLVAAKG HELDCLTETS GFRTFEVKEG LFFLNGNRYW LRGGNHIPFA
LAPNDLNLAN TFMQLMKAGN IDVTRTHTTP WNKLWMGAAD KNGIGVSFEG TWPWLMIHST
PLPDAKLIEM WKEEFLSLLK KYRNHPSLLF WTVNNEMKFY DNDNDLERAK EKYRVISDVV
KEMRRIDPTR PICFDSNYQA KGKKEKYGAD FMNTIDDGDI DDMHAYYNWY DYSLFRFFNG
EFQKRFKMVD RPLISQEMST GYPNNETGHP TRSYQLIHQN PQSLIGYECY DFSNPQSFLN
VQAFITGELA EALRRSNDQA SGIMHFALLT WFRQVYDYQK IEPYPTYYAL KRALQPILVS
AELWGRNLYG GEKLPTRIYV VNDREDGTDL KPTLLRWEIQ DETGKKLVWG DEELPAVKHY
GRYYIEPNIQ LPAKLPSNKV NAKLVLKLTE NGLPVSENEY NLLLADKKWN IGQVAENKKI
VLLDNDGTKA IFDFLRINYQ TVSSVREAVN SKQKADLCVI SGLTNCTEEE KEFLRGYQAK
GGKLLLLNSK EAAKIIYPEH ITGWIMPTEG DIVNMERNDA PVFDGIGVLE LRYFNNNKRE
IPTACNATLH VNRNENLTEL AGQMKIHAYI DGGAPEDRIN RINSMRGFTL IQIKDGRGIA
TVSTMCTEKA ETDPIAGKLL VNMINDLMK
//
