ID E3KAD9_PUCGT Unreviewed; 957 AA.
AC E3KAD9;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 2.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=DUF221-domain-containing protein {ECO:0008006|Google:ProtNLM};
GN ORFNames=PGTG_06917 {ECO:0000313|EMBL:EFP81296.2};
OS Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)
OS (Black stem rust fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia.
OX NCBI_TaxID=418459 {ECO:0000313|EMBL:EFP81296.2, ECO:0000313|Proteomes:UP000008783};
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CRL 75-36-700-3;
RG The Broad Institute Genome Sequencing Platform;
RA Birren B., Lander E., Galagan J., Nusbaum C., Devon K., Cuomo C., Jaffe D.,
RA Butler J., Alvarez P., Gnerre S., Grabherr M., Mauceli E., Brockman W.,
RA Young S., LaButti K., Sykes S., DeCaprio D., Crawford M., Koehrsen M.,
RA Engels R., Montgomery P., Pearson M., Howarth C., Larson L., White J.,
RA Zeng Q., Kodira C., Yandava C., Alvarado L., O'Leary S., Szabo L., Dean R.,
RA Schein J.;
RT "The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-
RT 700-3.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000008783}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CRL 75-36-700-3 / race SCCL
RC {ECO:0000313|Proteomes:UP000008783};
RX PubMed=21536894; DOI=10.1073/pnas.1019315108;
RA Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E.,
RA Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L.,
RA Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E.,
RA Goldberg J., Grabherr M.G., Kodira C.D., Kohler A., Kuees U.,
RA Lindquist E.A., Lucas S.M., Mago R., Mauceli E., Morin E., Murat C.,
RA Pangilinan J.L., Park R., Pearson M., Quesneville H., Rouhier N.,
RA Sakthikumar S., Salamov A.A., Schmutz J., Selles B., Shapiro H.,
RA Tanguay P., Tuskan G.A., Henrissat B., Van de Peer Y., Rouze P.,
RA Ellis J.G., Dodds P.N., Schein J.E., Zhong S., Hamelin R.C.,
RA Grigoriev I.V., Szabo L.J., Martin F.;
RT "Obligate biotrophy features unraveled by the genomic analysis of rust
RT fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family.
CC {ECO:0000256|ARBA:ARBA00007779}.
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DR EMBL; DS178278; EFP81296.2; -; Genomic_DNA.
DR RefSeq; XP_003325715.2; XM_003325667.2.
DR AlphaFoldDB; E3KAD9; -.
DR EnsemblFungi; EFP81296; EFP81296; PGTG_06917.
DR GeneID; 10536865; -.
DR KEGG; pgr:PGTG_06917; -.
DR VEuPathDB; FungiDB:PGTG_06917; -.
DR eggNOG; KOG1134; Eukaryota.
DR HOGENOM; CLU_009187_0_0_1; -.
DR InParanoid; E3KAD9; -.
DR OrthoDB; 1330110at2759; -.
DR Proteomes; UP000008783; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005227; F:calcium-activated cation channel activity; IBA:GO_Central.
DR InterPro; IPR045122; Csc1-like.
DR InterPro; IPR003864; CSC1/OSCA1-like_7TM.
DR InterPro; IPR027815; CSC1/OSCA1-like_cyt.
DR InterPro; IPR032880; Csc1/OSCA1-like_N.
DR PANTHER; PTHR13018:SF154; DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G11660)-RELATED; 1.
DR PANTHER; PTHR13018; PROBABLE MEMBRANE PROTEIN DUF221-RELATED; 1.
DR Pfam; PF14703; PHM7_cyt; 1.
DR Pfam; PF02714; RSN1_7TM; 1.
DR Pfam; PF13967; RSN1_TM; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000008783};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 31..52
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 391..414
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 434..460
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 481..507
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 544..566
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 587..606
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 612..630
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 650..673
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 31..185
FT /note="CSC1/OSCA1-like N-terminal transmembrane"
FT /evidence="ECO:0000259|Pfam:PF13967"
FT DOMAIN 210..374
FT /note="CSC1/OSCA1-like cytosolic"
FT /evidence="ECO:0000259|Pfam:PF14703"
FT DOMAIN 386..668
FT /note="CSC1/OSCA1-like 7TM region"
FT /evidence="ECO:0000259|Pfam:PF02714"
FT REGION 763..798
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 840..957
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 850..880
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 934..951
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 957 AA; 107512 MW; 2E5539B375813ED2 CRC64;
MSSTDTAASS AASGLLSQYQ AKLTQVSTSS VLIQLVLMFC CSVGTVLAFS ILRPKNKIVY
MPRYKYSAED KRPPKLEDGL FDWLKPLSKA TENELLAQIG LDAVVFLRFL RMCRWMCSLL
AMLACALLIP CDVFYNLKIM DKSQQLSTSS NTLAMVSISN VRGSWLYVHV VYGYLVTFIV
LYVIYVNYKT VVRLRWEWFR SPEYQNSIYS RSIMMTHVGS KHMSDSGLQN LLSQLQIPYP
TTAVHIGRRV GMLAFLIERH NQTVRDLEQV LVTYLKGGKI SPNRPTIRIG KNFLGLGGRK
VDAIDFYTAK IKQYELKIQA ARDAISGRKP ENYGFASFAA VAYAHIVAKK LGAKRIKGVA
FSLAPPPQDI IWENLIKSDI VVFRQRVIGE AFMTVIATFY VIPLVALALL ANLASLTQYV
GFLNSWSTAS PETFSAFAGI APPLLSTLLQ LALPVIMRAL ARFQAATTHQ KLDRAVFSRY
FAFLTATQFL IFSLLSVVFS TVAEIVVEIG KKESFLRILQ NFNKLPDKIQ STYLQQSNYW
LTWFPLRGFT SVLDLVQIVS LLLVFIRTKV WGRTPREIRE WTKPPEFDYP VYSANVLLML
LVAFVYAPLA PLVPLLASAS FFASSWIYKY QLMYISITRC ESGGRLWRML INRVLFALCA
MHAFLCLTIG LQLGWFKSIA TLPPFAIVIA FKFFLKSTFD SKFTWYIPTG AEFSQVHKHN
ADARKNRLMK RFGHPSLHAE LFTPMIHKDH QLLLSEVYRG RIGDPSEDPK NPGAQQNYGQ
ANYAGRSDDK APLSSGPQPV VGGLRFAAIA QHDLMYSQQQ FLRERGDDEM SLASTSLGNL
GHAREHSNAP APQNDLNIQG YLENGPRPSF QYQEKGGTGS RSASPAPHEY PPEFGSNVRL
PYHLPDDSYI PSPNPTHEES LEFDGYHPGI PASRHSTYGT SHSRSTSPLI TTRRPPA
//
