ID E3UMI2_9REOV Unreviewed; 310 AA.
AC E3UMI2;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 03-MAY-2023, entry version 35.
DE RecName: Full=Non-structural protein 3 {ECO:0000256|HAMAP-Rule:MF_04094};
DE Short=NSP3 {ECO:0000256|HAMAP-Rule:MF_04094};
DE AltName: Full=NCVP4 {ECO:0000256|HAMAP-Rule:MF_04094};
DE AltName: Full=Non-structural RNA-binding protein 34 {ECO:0000256|HAMAP-Rule:MF_04094};
DE Short=NS34 {ECO:0000256|HAMAP-Rule:MF_04094};
GN Name=NSP3 {ECO:0000313|EMBL:AHV80057.1};
GN ORFNames=L312_46533gpNSP3 {ECO:0000313|EMBL:AHZ32737.1},
GN L312_46643gpNSP3 {ECO:0000313|EMBL:AHZ32372.1}, L312_46644gpNSP3
GN {ECO:0000313|EMBL:AHZ32397.1}, L312_46646gpNSP3
GN {ECO:0000313|EMBL:AHZ32625.1}, L312_46649gpNSP3
GN {ECO:0000313|EMBL:AHZ33406.1}, L312_46698gpNSP3
GN {ECO:0000313|EMBL:AHZ32421.1}, L312_46703gpNSP3
GN {ECO:0000313|EMBL:AHZ32409.1}, L312_46704gpNSP3
GN {ECO:0000313|EMBL:AHZ33526.1}, L312_46711gpNSP3
GN {ECO:0000313|EMBL:AHZ32897.1}, L312_46718gpNSP3
GN {ECO:0000313|EMBL:AHZ33334.1}, L312_46719gpNSP3
GN {ECO:0000313|EMBL:AHZ32577.1}, L312_46720gpNSP3
GN {ECO:0000313|EMBL:AHZ91548.1}, L312_49820gpNSP3
GN {ECO:0000313|EMBL:AHZ91500.1}, NC78_46725gpNSP3
GN {ECO:0000313|EMBL:AKA40110.1}, W907_45292gpNSP3
GN {ECO:0000313|EMBL:AHV80534.1}, W907_45330gpNSP3
GN {ECO:0000313|EMBL:AHV80277.1}, W907_45334gpNSP3
GN {ECO:0000313|EMBL:AHV80485.1}, W907_45338gpNSP3
GN {ECO:0000313|EMBL:AHV80370.1}, W907_45339gpNSP3
GN {ECO:0000313|EMBL:AHV80214.1}, W907_45340gpNSP3
GN {ECO:0000313|EMBL:AHV80337.1}, W907_45341gpNSP3
GN {ECO:0000313|EMBL:AHV80094.1}, W907_45342gpNSP3
GN {ECO:0000313|EMBL:AHV80253.1}, W907_45343gpNSP3
GN {ECO:0000313|EMBL:AHV80057.1}, W907_45349gpNSP3
GN {ECO:0000313|EMBL:AHV80456.1};
OS Rotavirus A.
OC Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC Reovirales; Sedoreoviridae; Rotavirus.
OX NCBI_TaxID=28875 {ECO:0000313|EMBL:ADO20279.1, ECO:0000313|Proteomes:UP000222699};
RN [1] {ECO:0000313|Proteomes:UP000222699, ECO:0000313|Proteomes:UP000223298}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LB2744 {ECO:0000313|EMBL:ADO20279.1,
RC ECO:0000313|Proteomes:UP000222699}, and LB2772
RC {ECO:0000313|EMBL:ADO20281.1, ECO:0000313|Proteomes:UP000223298};
RX PubMed=21264876; DOI=10.1002/jmv.21977;
RA Banyai K., Mijatovic-Rustempasic S., Hull J.J., Esona M.D., Freeman M.M.,
RA Frace A.M., Bowen M.D., Gentsch J.R.;
RT "Sequencing and phylogenetic analysis of the coding region of six common
RT rotavirus strains: evidence for intragenogroup reassortment among co-
RT circulating G1P[8] and G2P[4] strains from the United States.";
RL J. Med. Virol. 83:532-539(2011).
RN [2] {ECO:0000313|Proteomes:UP000098428, ECO:0000313|Proteomes:UP000098588}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RVA/Human-wt/PRY/1005SR/2005/G2P[4] mixed_genotype
RC {ECO:0000313|EMBL:AHV80277.1}, RVA/Human-wt/PRY/1040SR/2005/G2P[4]
RC {ECO:0000313|EMBL:AHV80485.1}, RVA/Human-wt/PRY/1255SR/2005/G2G9P[4]
RC mixed_genotype {ECO:0000313|EMBL:AHV80370.1},
RC RVA/Human-wt/PRY/1297SR/2005/G2P[4] {ECO:0000313|EMBL:AHV80214.1},
RC RVA/Human-wt/PRY/1387SR/2006/G2G9P[4] mixed_genotype
RC {ECO:0000313|EMBL:AHV80337.1}, RVA/Human-wt/PRY/1388SR/2006/G2P[4]
RC {ECO:0000313|EMBL:AHV80094.1}, RVA/Human-wt/PRY/1395SR/2006/G2P[4]
RC {ECO:0000313|EMBL:AHV80253.1}, RVA/Human-wt/PRY/1463SR/2006/G2G9P[4]
RC mixed_genotype {ECO:0000313|EMBL:AHV80057.1},
RC RVA/Human-wt/PRY/1488SR/2007/G2P[4] {ECO:0000313|EMBL:AHV80456.1}, and
RC RVA/Human-wt/PRY/412/1999/G1G4P[4] mixed_genotype
RC {ECO:0000313|EMBL:AHV80534.1};
RA Kirkness E., Halpin R.A., Akopov A., Fedorova N., Tsitrin T., Stockwell T.,
RA Amedeo P., Bishop B., Edworthy P., Gupta N., Hoover J., Katzel D.,
RA Schobel S., Shrivastava S., Martinez M., Russomando G., Arbiza J.,
RA Wentworth D.E., Parra G.I.;
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000095875, ECO:0000313|Proteomes:UP000104860}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RVA/Human-wt/ZAF/MRC-DPRU11070/1999/G9P[6]
RC {ECO:0000313|EMBL:AHZ32409.1},
RC RVA/Human-wt/ZAF/MRC-DPRU1815/1999/G2P[6]
RC {ECO:0000313|EMBL:AHZ33406.1},
RC RVA/Human-wt/ZAF/MRC-DPRU1845/1999/G2P[6]
RC {ECO:0000313|EMBL:AHZ32625.1},
RC RVA/Human-wt/ZAF/MRC-DPRU2126/2003/G9P[6]
RC {ECO:0000313|EMBL:AHZ32737.1},
RC RVA/Human-wt/ZAF/MRC-DPRU3861/2000/G9P[X]
RC {ECO:0000313|EMBL:AHZ91548.1},
RC RVA/Human-wt/ZAF/MRC-DPRU4018/XXXX/G2P[6]
RC {ECO:0000313|EMBL:AHZ32397.1},
RC RVA/Human-wt/ZAF/MRC-DPRU4079-00/2000/G9P[6]
RC {ECO:0000313|EMBL:AHZ32421.1},
RC RVA/Human-wt/ZAF/MRC-DPRU421/2002/G9P[6]
RC {ECO:0000313|EMBL:AHZ33334.1},
RC RVA/Human-wt/ZAF/MRC-DPRU751/2006/G9P[6]
RC {ECO:0000313|EMBL:AHZ32897.1},
RC RVA/Human-wt/ZAF/MRC-DPRU774/2000/G9P[6]
RC {ECO:0000313|EMBL:AHZ33526.1},
RC RVA/Human-wt/ZAF/MRC-DPRU797/2000/G9P[6]
RC {ECO:0000313|EMBL:AHZ32577.1}, and
RC RVA/Human-wt/ZAF/MRC-DPRU800/XXXX/G9P[6]
RC {ECO:0000313|EMBL:AHZ91500.1};
RA Wentworth D.E., Halpin R.A., Stucker K.M., Akopov A., Fedorova N.,
RA Tsitrin T., Puri V., Stockwell T., Amedeo P., Bishop B., Gupta N.,
RA Hoover J., Katzel D., Schobel S., Shrivastava S., Nyaga M.M.,
RA Magagula N.B., Peenze I., Seheri M.L., Mphahlele J., Steele A.D.,
RA Mwenda M.J.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:AHZ32372.1, ECO:0000313|Proteomes:UP000153356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RVA/Human-wt/GHA/MRC-DPRU1818/1999/G2P[6]
RC {ECO:0000313|EMBL:AHZ32372.1};
RA Wentworth D.E., Halpin R.A., Stucker K.M., Akopov A., Fedorova N.,
RA Tsitrin T., Puri V., Stockwell T., Amedeo P., Bishop B., Gupta N.,
RA Hoover J., Katzel D., Schobel S., Shrivastava S., Nyaga M.M.,
RA Magagula N.B., Peenze I., Seheri M.L., Mwenda J., Mphahlele J.,
RA Steele A.D., Mwenda M.J.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:AKA40110.1, ECO:0000313|Proteomes:UP000223807}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RVA/Human-wt/ZAF/MRC-DPRU11051/1999/G9P[6]
RC {ECO:0000313|EMBL:AKA40110.1, ECO:0000313|Proteomes:UP000223807};
RA Das S.R., Halpin R.A., Stucker K.M., Akopov A., Fedorova N., Puri V.,
RA Stockwell T., Amedeo P., Katzel D., Schobel S., Shrivastava S., Nyaga M.M.,
RA Magagula N.B., Peenze I., Seheri M.L., Wentworth D.E., Mphahlele J.;
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:QXY09208.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=RVA/Human-wt/KEN/KLF0584/2012/G8P[4]
RC {ECO:0000313|EMBL:QXY09208.1};
RA Lambisia A.W., Mwanga M.J., Makori T.O., Mudibo E.O., de Laurent Z.R.,
RA Lewa C.S., Cheruiyot R., Mutunga M., Nokes J.D., Agoti C.N.;
RT "Rotavirus group A genomic surveillance before and after vaccine
RT introduction in Kilifi, Kenya.";
RL Submitted (MAY-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays an important role in stimulating the translation of
CC viral mRNAs. These mRNAs are capped but not polyadenylated, instead
CC terminating in a conserved sequence 'GACC' at the 3' that is recognized
CC by NSP3, which competes with host PABPC1 for EIF4G1 binding. The
CC interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1
CC interaction, thereby facilitating the initiation of capped mRNA
CC translation. {ECO:0000256|HAMAP-Rule:MF_04094}.
CC -!- SUBUNIT: Homodimer. Interacts (via the coiled-coil region) with host
CC ZC3H7B (via LD motif). Interacts with host EIF4G1. {ECO:0000256|HAMAP-
CC Rule:MF_04094}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000256|HAMAP-Rule:MF_04094}.
CC -!- SIMILARITY: Belongs to the rotavirus A NSP3 protein family.
CC {ECO:0000256|HAMAP-Rule:MF_04094}.
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DR EMBL; HM467973; ADO20279.1; -; Genomic_RNA.
DR EMBL; HM467975; ADO20281.1; -; Genomic_RNA.
DR EMBL; KJ626567; AHV80057.1; -; Genomic_RNA.
DR EMBL; KJ626601; AHV80094.1; -; Genomic_RNA.
DR EMBL; KJ626712; AHV80214.1; -; Genomic_RNA.
DR EMBL; KJ626748; AHV80253.1; -; Genomic_RNA.
DR EMBL; KJ626770; AHV80277.1; -; Genomic_RNA.
DR EMBL; KJ626826; AHV80337.1; -; Genomic_RNA.
DR EMBL; KJ626856; AHV80370.1; -; Genomic_RNA.
DR EMBL; KJ626935; AHV80456.1; -; Genomic_RNA.
DR EMBL; KJ626962; AHV80485.1; -; Genomic_RNA.
DR EMBL; KJ627007; AHV80534.1; -; Genomic_RNA.
DR EMBL; KJ751621; AHZ32372.1; -; Genomic_RNA.
DR EMBL; KJ751644; AHZ32397.1; -; Genomic_RNA.
DR EMBL; KJ751655; AHZ32409.1; -; Genomic_RNA.
DR EMBL; KJ751666; AHZ32421.1; -; Genomic_RNA.
DR EMBL; KJ751809; AHZ32577.1; -; Genomic_RNA.
DR EMBL; KJ751853; AHZ32625.1; -; Genomic_RNA.
DR EMBL; KJ751957; AHZ32737.1; -; Genomic_RNA.
DR EMBL; KJ752103; AHZ32897.1; -; Genomic_RNA.
DR EMBL; KJ752505; AHZ33334.1; -; Genomic_RNA.
DR EMBL; KJ752571; AHZ33406.1; -; Genomic_RNA.
DR EMBL; KJ752681; AHZ33526.1; -; Genomic_RNA.
DR EMBL; KJ753267; AHZ91500.1; -; Genomic_RNA.
DR EMBL; KJ753311; AHZ91548.1; -; Genomic_RNA.
DR EMBL; KP752457; AKA40110.1; -; Genomic_RNA.
DR EMBL; MZ094052; QXY09208.1; -; Genomic_RNA.
DR Proteomes; UP000095875; Genome.
DR Proteomes; UP000098428; Genome.
DR Proteomes; UP000098588; Genome.
DR Proteomes; UP000104860; Genome.
DR Proteomes; UP000105026; Genome.
DR Proteomes; UP000105692; Genome.
DR Proteomes; UP000110524; Genome.
DR Proteomes; UP000117880; Genome.
DR Proteomes; UP000120466; Genome.
DR Proteomes; UP000123731; Genome.
DR Proteomes; UP000125457; Genome.
DR Proteomes; UP000128534; Genome.
DR Proteomes; UP000132347; Genome.
DR Proteomes; UP000133895; Genome.
DR Proteomes; UP000135279; Genome.
DR Proteomes; UP000142235; Genome.
DR Proteomes; UP000148795; Genome.
DR Proteomes; UP000153356; Genome.
DR Proteomes; UP000155083; Genome.
DR Proteomes; UP000160303; Genome.
DR Proteomes; UP000164648; Genome.
DR Proteomes; UP000175457; Genome.
DR Proteomes; UP000222699; Genome.
DR Proteomes; UP000223298; Genome.
DR Proteomes; UP000223807; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR CDD; cd20714; NSP3_rotavirus; 1.
DR Gene3D; 3.30.70.1610; -; 1.
DR Gene3D; 1.20.5.970; Nonstructural RNA-binding protein; 1.
DR Gene3D; 6.10.280.20; Rotavirus non-structural protein NSP3, N-terminal domain; 1.
DR HAMAP; MF_04094; ROTA_A_NSP3; 1.
DR HAMAP; MF_04090; ROTA_NSP3; 1.
DR InterPro; IPR042519; NSP3_N_rotavirus.
DR InterPro; IPR036082; NSP3_sf.
DR InterPro; IPR002873; Rotavirus_NSP3.
DR Pfam; PF01665; Rota_NSP3; 1.
DR SUPFAM; SSF69903; NSP3 homodimer; 1.
DR SUPFAM; SSF58030; Rotavirus nonstructural proteins; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|HAMAP-Rule:MF_04094};
KW Host cytoplasm {ECO:0000256|HAMAP-Rule:MF_04094};
KW Host-virus interaction {ECO:0000256|HAMAP-Rule:MF_04094};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_04094};
KW Translation regulation {ECO:0000256|ARBA:ARBA00022845, ECO:0000256|HAMAP-
KW Rule:MF_04094}.
FT REGION 1..146
FT /note="RNA-binding"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
FT REGION 147..203
FT /note="Dimerization"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
FT REGION 167..231
FT /note="Interaction with host ZC3H7B"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
FT REGION 205..310
FT /note="Interaction with host EIF4G1"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
FT COILED 163..234
FT /evidence="ECO:0000256|HAMAP-Rule:MF_04094"
SQ SEQUENCE 310 AA; 35978 MW; 9451322FE6607412 CRC64;
MESTQQMASS IINSSFEAAV VAATSTLELM GIQYDYNEVY TRVKSKFDFV MDDSGVKNNL
IGKAATIDQA LNGKFSSSIR NRNWMTDSKT VARLDEDVNK LRLLLSSKGI DQKMRVLNAC
FSVKRVPGKS SSIIKCTRLM KEKIERGEVE VDDAFIEEKM EIDTIDWKSR YDQLERRFES
LKQRVNEKYN NWVIKARKVN ENMNSLQNVI SQQQAHINEL QIYNNKLERD LQSKIGSVIS
SIEWYLRSME LSDDIKSDIE QQLNSIDHIN PVNAFDDFES ILRNLISDYD RIFIMFKGLL
QQSNYTYTYE
//
