ID E3VXA4_AQUSE Unreviewed; 552 AA.
AC E3VXA4;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN Name=aseIM {ECO:0000313|EMBL:ADO24185.1};
OS Aquaspirillum serpens.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
OC Aquaspirillaceae; Aquaspirillum.
OX NCBI_TaxID=190 {ECO:0000313|EMBL:ADO24185.1};
RN [1] {ECO:0000313|EMBL:ADO24185.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NEB 448 {ECO:0000313|EMBL:ADO24185.1};
RA Stewart F., Morgan R.D.;
RT "AseI restriction-modification system genes.";
RL Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; HQ327700; ADO24185.1; -; Genomic_DNA.
DR AlphaFoldDB; E3VXA4; -.
DR REBASE; 3285; M.AseI.
DR PRO; PR:E3VXA4; -.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR025931; TaqI_C.
DR PANTHER; PTHR33841:SF1; ADENINE-SPECIFIC METHYLTRANSFERASE PGLX; 1.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF12950; TaqI_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 105..280
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 406..519
FT /note="TaqI-like C-terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF12950"
SQ SEQUENCE 552 AA; 62978 MW; A169844B74C7254C CRC64;
MGMHEMRNME QQTFLDIENL EYFSIEESAK ILGVSSATIR NWVKTGYLEI ISKGKISRNS
VENFDKKISG VEKLNQRANK SKKDNHNHEY LAAEFLRKID LGIEKIDSMG DLYESGLSDS
YRNKEGIYYT PSYIVNDLLS LKNQLTGDET FCDPCCGSGN FLVRALEIGF KPENIFGFDT
DHVAVEIAKR RILEKTGYKS NNIKCYDFLH LSVSEKAGSY DYIFTNPPWG KKIEQAEKKS
ISKILKGGSS IDTCSLFYFC CIKSLNLGGT LGLLLPESFF NVATYEDARL SALMLQIQRL
SHYGKPFKGI LTGAVGMVIS KKDSILDQNI DCVYKDKNFK RNVDSFRKNP KSIFNISCTD
DEAKVIEHVY SIPYVTLRGR AQWGIGIVTG NNKKYVDNIL KDGLIPVYKG SDITKYGLKP
ASTYIPKDFS LYQQVAPINL YEAPEKLIYK FISSNLCFFY DTESRFVINS ANILITEKEF
PVSMKVICEL FNSDFMNWIF RKLFNTHKVL RGDLELLPIH DQFLSGKNFI ESEYLTSINV
ERTNCGTFRI KK
//
