ID E4KMU1_9LACT Unreviewed; 1181 AA.
AC E4KMU1;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:EFR31790.1};
GN ORFNames=HMPREF9257_0154 {ECO:0000313|EMBL:EFR31790.1};
OS Eremococcus coleocola ACS-139-V-Col8.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Aerococcaceae; Eremococcus.
OX NCBI_TaxID=908337 {ECO:0000313|EMBL:EFR31790.1, ECO:0000313|Proteomes:UP000005990};
RN [1] {ECO:0000313|EMBL:EFR31790.1, ECO:0000313|Proteomes:UP000005990}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ACS-139-V-Col8 {ECO:0000313|EMBL:EFR31790.1,
RC ECO:0000313|Proteomes:UP000005990};
RA Durkin A.S., Madupu R., Torralba M., Gillis M., Methe B., Sutton G.,
RA Nelson K.E.;
RL Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFR31790.1}.
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DR EMBL; AENN01000006; EFR31790.1; -; Genomic_DNA.
DR RefSeq; WP_006417922.1; NZ_AENN01000006.1.
DR AlphaFoldDB; E4KMU1; -.
DR STRING; 908337.HMPREF9257_0154; -.
DR eggNOG; COG1197; Bacteria.
DR Proteomes; UP000005990; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000005990}.
FT DOMAIN 639..800
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 821..975
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1181 AA; 134438 MW; DC88EE1D4D2A7EA6 CRC64;
MQVELNRNAF QESIQQINAD KKRKLPYLIT GLDGSARAIY LAQLFQARPT TILIVESSDL
QMQELSQDLA HILPEVPVLT YPADDNLAIE YAQASMDFAS QRVAALNFLA QGQPGFVLTT
LAGLRQKLNP VDQWVAYNKI IQVGQELDRQ ALLDTLIALS YKSVNMVMTP GEFSVRGGIV
DFYPLDSDYP VRLDFFDSEV DSIRYFNAET QESVEQIDEV ILSPVQELIF PNFMQLMVAD
ELKKRAKKYL DKVKDPAIKN NLQINLLGDL KNLADHNEPL KYASAFFGLI PETSSLLDYL
RYDGHLVIND YAKLQQEQVQ QIEQNHYWIE QEIEKGQLIP NLPIKWDFED LVKNFSGSTL
HFSVLQRGYV AMALAGLFNY KYQTMTPYFS QMPMIIDDLQ TWLKQGYVVQ VALPSKKQVL
NFRQALADHG FDKVYLQDEQ PIQANRVNLV TLDLSKGFVL SEQAWALVTH HDLFKEVNRP
RGRVRQPHLS NAEKIKSYNE LSVGDYVVHL NHGVGRYTGM ETLEMNGVHR DMLAIEYQNN
ARVLIPVDKI HLIQKYVSSE SKTPKINKLG GTEWAKTKQK VQATVEDIAD DLIKLYAKRE
AEKGYRFSPD TPEQSEFEQA FGFVETPDQL TSSQEIKADM EKSRPMDRLL VGDVGYGKTE
VAMRAIFKAV MDGKQVAFLV PTTVLAQQHY NTLQARFNDW PFEIGLLSRF VSRVQQKETI
AKLKSGAVSI VVGTHRILSK DIVFNDLGLL IVDEEQRFGV KHKERLKQLK AQVDVLTLTA
TPIPRTLHMS MIGIRDLSVI ETPPNNRFPV QTYIMERNEG AIKTAIEREM ARGGQCFYLY
NRVATIYQRA DEISQLVPQA RVAVAHGQMS ETELETVLVD FIQGLYDVLV TTTIIETGVD
IPNANTLFVD HADKMGLSTL YQLRGRVGRT HRVAYAYLMY EPFKQLSEVS EKRLKAIREF
TDLGSGFKIA MRDLSIRGAG NLLGKQQSGF IDSVGYDLYT QMLKEAVDQR KGIKKKQSQY
ESQAIEWNVD VDAYLPKDYI QDERQKINMY KAIQQIDSEE AYRSIQDQLI DRFGEFPDQV
ADLLDIALIK YYGLKSGIEE IKQDRQRIVV TYNERASMKL KGPNIFQALQ EVPLKAEVTV
NGGKLAIRLL VQGKVSYQWL PALKALAKES MSIVNQGEEQ E
//
