ID E4LB44_9FIRM Unreviewed; 494 AA.
AC E4LB44;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE SubName: Full=23S rRNA (Uracil-5-)-methyltransferase RumA {ECO:0000313|EMBL:EFR61098.1};
DE EC=2.1.1.- {ECO:0000313|EMBL:EFR61098.1};
GN Name=rumA {ECO:0000313|EMBL:EFR61098.1};
GN ORFNames=HMPREF9199_0682 {ECO:0000313|EMBL:EFR61098.1};
OS Veillonella sp. oral taxon 158 str. F0412.
OC Bacteria; Bacillota; Negativicutes; Veillonellales; Veillonellaceae;
OC Veillonella.
OX NCBI_TaxID=879309 {ECO:0000313|EMBL:EFR61098.1, ECO:0000313|Proteomes:UP000003565};
RN [1] {ECO:0000313|EMBL:EFR61098.1, ECO:0000313|Proteomes:UP000003565}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0412 {ECO:0000313|EMBL:EFR61098.1,
RC ECO:0000313|Proteomes:UP000003565};
RA Durkin A.S., Madupu R., Torralba M., Gillis M., Methe B., Sutton G.,
RA Nelson K.E.;
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFR61098.1}.
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DR EMBL; AENU01000003; EFR61098.1; -; Genomic_DNA.
DR RefSeq; WP_009372064.1; NZ_AENU01000003.1.
DR AlphaFoldDB; E4LB44; -.
DR STRING; 879309.HMPREF9199_0682; -.
DR eggNOG; COG2265; Bacteria.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000003565; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 44..102
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 449
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 324
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 353
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 374
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 422
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 494 AA; 54513 MW; 786264407456AA0A CRC64;
MNGTGRTSRK HKSKQQNRRG ARSNQGQITS GTVSKKQQAI LNAPVKVGDE VLVTIHGIGS
SGEGVGRVDD FTVFVPFALP EETVKVAIDM VKKTYATGRL LEIVTMAPNR IDASCDLYGF
CGGCQLQHIT YEGQLSLKTQ KVKDVIERIG HQNPDLVKPA LGPKEPWAYR NKMQMPVGGT
KGDIQMGFYA MGSHDIVQGT NCPIQDEGNN IIAQACYQIA KEFDIEPYDE HTGNGILRHV
IGRIGQSGWM IILVTATDHL PHQEKWVEKL TERIPQVETI VHNVNGKRTN VILGPKNNIL
YGDGTITDHI KDLRFTLSPH SFFQVNPEQT TVLYDQALAY ADLKGDETVI DAYCGTGTIS
LFLAHKAKHV IGIEIVEPAI INARENAKCN GYDNTEFIVA DAAVEMPKLY KAGVRADVIV
FDPIRAGCKE EVLTSAAGME PKRIVYVSCN PATMARDIEI LTHYGYELKE VQPVDMFPMT
AHVETVCLLS RKDK
//
