ID E4MT81_CAPOC Unreviewed; 469 AA.
AC E4MT81;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE SubName: Full=Alpha amylase, catalytic domain protein {ECO:0000313|EMBL:EFS97101.1};
GN Name=amyA2 {ECO:0000313|EMBL:EFS97101.1};
GN ORFNames=HMPREF1977_1591 {ECO:0000313|EMBL:EFS97101.1};
OS Capnocytophaga ochracea F0287.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Capnocytophaga.
OX NCBI_TaxID=873517 {ECO:0000313|EMBL:EFS97101.1, ECO:0000313|Proteomes:UP000005391};
RN [1] {ECO:0000313|EMBL:EFS97101.1, ECO:0000313|Proteomes:UP000005391}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0287 {ECO:0000313|EMBL:EFS97101.1,
RC ECO:0000313|Proteomes:UP000005391};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFS97101.1}.
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DR EMBL; AEOH01000041; EFS97101.1; -; Genomic_DNA.
DR RefSeq; WP_002674126.1; NZ_GL573160.1.
DR AlphaFoldDB; E4MT81; -.
DR eggNOG; COG0366; Bacteria.
DR HOGENOM; CLU_024572_3_0_10; -.
DR Proteomes; UP000005391; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 2.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}.
FT DOMAIN 43..388
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 236
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 260
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 201
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 469 AA; 53777 MW; AD348518550AFEB9 CRC64;
MKYYIYLLAG LLAFSCDKKS NNDNPEPQNT VPLDLTKIDN GNRVMMQAFY WDVTPLGEWW
NTITPKLTEW KANGVDRIWL PPASKGASGG LSMGYDPSDY FDFGEYFQHE TTKTRFGSRA
ELENLISKAH ESGLQVIADI VIGHNNGGGK EWNPYRNKET YTLFDETHGN ASGRFNRNYE
CFHPNKYATS DEEENFYPER DLSHKVPYVR EELWEKDNSM AKYYKNTMKF DGWRFDYVKS
FGAWVVRDWL KAVGGFAVGE LWDGNPETLK KWVDESGASA FDFACFYALE KALDRNKDMH
ELMTDTHPML RTLRTEKAVT FTANHDTEKD KIADNTIAPD KKLMAYAYIL THSGYPCIFY
SDYENEAFKA KLQKLMLINR SLAVGEEKFH LASNTEYVAS RLGNEKSPGL VLFINNSTLP
AKRTITTHWK NKTLMDYSGN SHLFFVTDSD GNVTIQVPAN SYTVWSIGK
//
