ID   E5B7Z9_ERWAM            Unreviewed;       344 AA.
AC   E5B7Z9;
DT   08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT   08-FEB-2011, sequence version 1.
DT   27-MAR-2024, entry version 34.
DE   SubName: Full=Putative tRNA/rRNA methyltransferase {ECO:0000313|EMBL:CBX81604.1};
DE            EC=2.1.1.- {ECO:0000313|EMBL:CBX81604.1};
GN   Name=yfiF {ECO:0000313|EMBL:CBX81604.1};
GN   ORFNames=EAIL5_2784 {ECO:0000313|EMBL:CBX81604.1};
OS   Erwinia amylovora ATCC BAA-2158.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Erwinia.
OX   NCBI_TaxID=889211 {ECO:0000313|EMBL:CBX81604.1};
RN   [1] {ECO:0000313|EMBL:CBX81604.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC BAA-2158 {ECO:0000313|EMBL:CBX81604.1};
RA   Powney R., Smits T.H., Sawbridge T., Frey B., Blom J., Frey J.E.,
RA   Plummer K.M., Beer S.V., Luck J., Duffy B., Rodoni B.;
RT   "Genome Sequence of an Erwinia amylovora Strain with Pathogenicity
RT   Restricted to Rubus Plants.";
RL   J. Bacteriol. 193:785-786(2011).
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DR   EMBL; FR719194; CBX81604.1; -; Genomic_DNA.
DR   AlphaFoldDB; E5B7Z9; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   CDD; cd18095; SpoU-like_rRNA-MTase; 1.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR029064; Ribosomal_eL30-like_sf.
DR   InterPro; IPR004441; rRNA_MeTrfase_TrmH.
DR   InterPro; IPR001537; SpoU_MeTrfase.
DR   InterPro; IPR013123; SpoU_subst-bd.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   InterPro; IPR016479; YfiF_prd.
DR   PANTHER; PTHR46429; 23S RRNA (GUANOSINE-2'-O-)-METHYLTRANSFERASE RLMB; 1.
DR   PANTHER; PTHR46429:SF2; TRNA_RRNA METHYLTRANSFERASE-RELATED; 1.
DR   Pfam; PF00588; SpoU_methylase; 1.
DR   Pfam; PF08032; SpoU_sub_bind; 1.
DR   PIRSF; PIRSF006280; YfiF_prd; 2.
DR   SMART; SM00967; SpoU_sub_bind; 1.
DR   SUPFAM; SSF75217; alpha/beta knot; 1.
DR   SUPFAM; SSF55315; L30e-like; 1.
PE   4: Predicted;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000313|EMBL:CBX81604.1};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CBX81604.1}.
FT   DOMAIN          109..184
FT                   /note="RNA 2-O ribose methyltransferase substrate binding"
FT                   /evidence="ECO:0000259|SMART:SM00967"
FT   REGION          1..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..28
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        35..67
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   344 AA;  37263 MW;  D2C98E9FDBEA6815 CRC64;
     MNDELKGKSN KVKVMYVRSD DENSKSGKNP RTGKGGYRQD GERRPARNAD SKPRGGRDRD
     EGRSDSPWRT VSRAPNAAAA DDKPDHGGIS GKSFINPEQL RRQRMEETRV YGENACQALF
     QSRPESIVRA WFLQSVTPRF REALKWMAAN RKAYHTVDDA ELQKASGTEH HGGVCFLIKK
     RVGMPVSEWL AQSGQKDCVV ALEDIGNPHN LGGIMRSCAH FGAKGLLVDD ASLLESGAAV
     RTAEGGAEHV QAISGESFAA GLDAFRQAGY TIVTTSSHQG VPLSSAELPA RMVLVLGQER
     DGLSDSSFQR GDLSVSIGGT GNVESLNVSV ATGVLLAEWW RQNA
//