ID E5Q8U0_GEOSE Unreviewed; 389 AA.
AC E5Q8U0;
DT 08-FEB-2011, integrated into UniProtKB/TrEMBL.
DT 08-FEB-2011, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=site-specific DNA-methyltransferase (cytosine-N(4)-specific) {ECO:0000256|ARBA:ARBA00012185};
DE EC=2.1.1.113 {ECO:0000256|ARBA:ARBA00012185};
GN Name=bsrIM2 {ECO:0000313|EMBL:ADR72995.1};
OS Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=1422 {ECO:0000313|EMBL:ADR72995.1};
RN [1] {ECO:0000313|EMBL:ADR72995.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NEBM140 {ECO:0000313|EMBL:ADR72995.1};
RA Lunnen K.D., Wilson G.G.;
RT "BsrI restriction-modification system genes.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC Evidence={ECO:0000256|ARBA:ARBA00001893};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC subfamily. {ECO:0000256|ARBA:ARBA00010203}.
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DR EMBL; HQ636105; ADR72995.1; -; Genomic_DNA.
DR AlphaFoldDB; E5Q8U0; -.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR002941; DNA_methylase_N4/N6.
DR InterPro; IPR017985; MeTrfase_CN4_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF01555; N6_N4_Mtase; 2.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00093; N4_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 22..81
FT /note="DNA methylase N-4/N-6"
FT /evidence="ECO:0000259|Pfam:PF01555"
FT DOMAIN 239..348
FT /note="DNA methylase N-4/N-6"
FT /evidence="ECO:0000259|Pfam:PF01555"
SQ SEQUENCE 389 AA; 45556 MW; 6E3296F9B5EED359 CRC64;
MVDITAIQNL KIFEEDYSNT TDLNHGIHSY PAKFPPQIPG KLLDKFAKDN YVVLDPFCGS
GTTLVEASLR NLDSVGNDIN PIALLISTVK TTKYFDTDFE ELEKIIYDLK DDYMNNKNNL
KSTIDFPNKD HWFQKNVQKE IELILKHINL CSNEKYRNLL KVVLSEIIVT VSNQESDTRY
AAIDKNIPDG KTIELFEKRY FAIKDKILSF SQMVKDFEYE TKIISNDARN LIDIRSESID
IIITSPPYAN TYDYYLYHKH RMNWLGYNFK ETQNIEIGSR NEYSSKKQKP EKWKHDLMLV
LQEMYRVMKK DRLCFIIIGD SVINKEHIKI NDVIREIATK IGFEYLNEES VPLSKNSRKF
NKKFGTDQTK LEHLISLYKP KLTIIMKAH
//
