ID   E5VDU6_9BACE            Unreviewed;       981 AA.
AC   E5VDU6;
DT   08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT   08-MAR-2011, sequence version 1.
DT   27-MAR-2024, entry version 39.
DE   RecName: Full=Gluconolaconase {ECO:0008006|Google:ProtNLM};
GN   ORFNames=HMPREF1007_02930 {ECO:0000313|EMBL:EFV24936.1};
OS   Bacteroides sp. 4_1_36.
OC   Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Bacteroides.
OX   NCBI_TaxID=457393 {ECO:0000313|EMBL:EFV24936.1, ECO:0000313|Proteomes:UP000004735};
RN   [1] {ECO:0000313|EMBL:EFV24936.1, ECO:0000313|Proteomes:UP000004735}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=4_1_36 {ECO:0000313|EMBL:EFV24936.1,
RC   ECO:0000313|Proteomes:UP000004735};
RG   The Broad Institute Genome Sequencing Platform;
RA   Ward D., Earl A., Feldgarden M., Young S.K., Gargeya S., Zeng Q.,
RA   Alvarado L., Berlin A., Bochicchio J., Chapman S.B., Chen Z., Freedman E.,
RA   Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D.,
RA   Howarth C., Mehta T., Neiman D., Pearson M., Roberts A., Saif S., Shea T.,
RA   Shenoy N., Sisk P., Stolte C., Sykes S., White J., Yandava C.,
RA   Allen-Vercoe E., Sibley C., Strauss J., Daigneault M., Haas B., Nusbaum C.,
RA   Birren B.;
RT   "The Genome Sequence of Bacteroides sp. strain 4_1_36.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC         residues in alpha-D-galactosides, including galactose
CC         oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC         Evidence={ECO:0000256|ARBA:ARBA00001255};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-
CC         galactosidic residues, producing free D-galactose.; EC=3.2.1.n1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001271};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EFV24936.1}.
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DR   EMBL; ACTC01000110; EFV24936.1; -; Genomic_DNA.
DR   AlphaFoldDB; E5VDU6; -.
DR   HOGENOM; CLU_303632_0_0_10; -.
DR   Proteomes; UP000004735; Unassembled WGS sequence.
DR   GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 2.160.20.10; Single-stranded right-handed beta-helix, Pectin lyase-like; 2.
DR   Gene3D; 2.120.10.30; TolB, C-terminal domain; 2.
DR   InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR   InterPro; IPR024535; Pectate_lyase_SF_prot.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR013658; SGL.
DR   PANTHER; PTHR47572:SF4; LACTONASE DRP35; 1.
DR   PANTHER; PTHR47572; LIPOPROTEIN-RELATED; 1.
DR   Pfam; PF12708; Pectate_lyase_3; 2.
DR   Pfam; PF08450; SGL; 1.
DR   SUPFAM; SSF63829; Calcium-dependent phosphotriesterase; 1.
DR   SUPFAM; SSF51126; Pectin lyase-like; 2.
PE   4: Predicted;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801}.
FT   DOMAIN          29..238
FT                   /note="Pectate lyase superfamily protein"
FT                   /evidence="ECO:0000259|Pfam:PF12708"
FT   DOMAIN          356..409
FT                   /note="Pectate lyase superfamily protein"
FT                   /evidence="ECO:0000259|Pfam:PF12708"
FT   DOMAIN          920..972
FT                   /note="SMP-30/Gluconolactonase/LRE-like region"
FT                   /evidence="ECO:0000259|Pfam:PF08450"
FT   REGION          484..515
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   981 AA;  109712 MW;  A8AF934E2810A082 CRC64;
     MPSGWAAEGG SAYTQRPDDP EAFYFTPENY GFKADGKSDV TDALQEAINQ VKREKNFGIL
     FLPEGNYRIS KTIQIPSSIR LIGYGKKRPV IYLGADTPGF QTTQNYMIWF TGGLAQEGRK
     PSDAGAGTFY SAVSNVDFRI DKGNPQAVAI RAHFAQHGFI NHCDIRIGSG KAGMYDVGNE
     LEDVRFYGGE YGIISSRTSP GWPMMMVDTY FEGQRKAAVY SKEVGFAIVN MHVKNTPVAF
     EMAENLADRL HVENSLWENI SEAGVRVSVE GNTFSQLNLV NVDCRNVPVL VGYAQSGKKV
     AGKAKMYRVK EFTYGLVYQD LNDASSFREI CEIEPVAKLP VTLGKDLPVL PAMETWVNIR
     DLGAKGDGKT DDTEVFEKAV SLHKNIYVPQ GWYRLTRTLK LSPGTKLIGL HPFGTQFLLK
     ESEPAFSGFG VPVPLVESSE GGDDMLNGIG INTGAYNYRA VGCKWMAGER SYLNDVKFVG
     GHGTLRKPAP NASGQSSYRR DERRISSPSS PVMETGKDMA WDNQYWSLWI TNNGGGTIKD
     VWTASTYAAS GLYISETKTP GRIYAMSLEH HVRTEARFHN VANWKIYAFQ FEEEGREGPD
     CYMAEMSNCQ NIEMVNVWMY RVIRAFMPKR IGFRIWDCKN ITFRNMHNYT QILPVIEFPI
     YDMNKKLPVY SWDFARLTVS GSEKSLRPSC TVMDKPVKLA TGFELASGAT TDSKGNIYFC
     ENRLKKIYKW SADTEQITLI ADYPWKPFTL ATDTQDNLLV IFRYDPQPGY LVNGKQETVV
     RLPDDNPMYS GWGNSGWSAW GYSFNPDNVD ATMKPMPRVV TASMKNIQKV IHPSSRWRGD
     FDKVVASMPE YSFVAPDGVT IIPDTYDLGR SCALTVTVPG QSEPVYIVNE MNKTTVQLSV
     GVDGRLTEQG IICPYGQYSN VTDADGNVYV ADGEIFVYDK SGKEQRRIQI EERPISLAIG
     GRQKDMLFVT TSSSFYGIKI R
//