ID E6K3N0_9BACT Unreviewed; 346 AA.
AC E6K3N0;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE SubName: Full=Glycosyl hydrolase, family 43 {ECO:0000313|EMBL:EFU31798.1};
DE EC=3.2.1.99 {ECO:0000313|EMBL:EFU31798.1};
GN ORFNames=HMPREF6485_0183 {ECO:0000313|EMBL:EFU31798.1};
OS Segatella buccae ATCC 33574.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC Segatella.
OX NCBI_TaxID=873513 {ECO:0000313|EMBL:EFU31798.1, ECO:0000313|Proteomes:UP000003112};
RN [1] {ECO:0000313|EMBL:EFU31798.1, ECO:0000313|Proteomes:UP000003112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33574 {ECO:0000313|EMBL:EFU31798.1,
RC ECO:0000313|Proteomes:UP000003112};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834, ECO:0000256|PIRNR:PIRNR026534}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|PIRNR:PIRNR026534}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFU31798.1}.
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DR EMBL; AEPD01000006; EFU31798.1; -; Genomic_DNA.
DR RefSeq; WP_004344058.1; NZ_GL586311.1.
DR AlphaFoldDB; E6K3N0; -.
DR STRING; 873513.HMPREF6485_0183; -.
DR eggNOG; COG3507; Bacteria.
DR HOGENOM; CLU_009397_5_1_10; -.
DR UniPathway; UPA00667; -.
DR Proteomes; UP000003112; Unassembled WGS sequence.
DR GO; GO:0046558; F:arabinan endo-1,5-alpha-L-arabinosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0031222; P:arabinan catabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR016840; Glyco_hydro_43_endo_a_Ara-ase.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR PIRSF; PIRSF026534; Endo_alpha-L-arabinosidase; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR026534};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR026534};
KW Reference proteome {ECO:0000313|Proteomes:UP000003112};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..346
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003206844"
FT ACT_SITE 41
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-1"
FT ACT_SITE 227
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-1"
FT BINDING 41
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT BINDING 119
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT BINDING 156..159
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT BINDING 176..178
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-2"
FT SITE 159
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-3"
FT SITE 298
FT /note="Important for substrate recognition"
FT /evidence="ECO:0000256|PIRSR:PIRSR026534-3"
SQ SEQUENCE 346 AA; 38954 MW; 074C9CCA9442EF8A CRC64;
MKRPLFLMLF FAATAITLAQ PDGRSHGGFD TFVTDTPMVH DPVMAFENGR YYLFSTGHGI
RQMVSSDRRS WTVSRHPALD SIPAWTRDSV PGFAIHVWAP DIIRWHGRWW MAYSCSTFGK
NTSAIGLMSR SSLSDSVGWK DEGPLVCSRE GRDNWNAIDP NFIIDEHDRP WLVWGSFWDG
IQLVRLDSTL HIASGEHPHT IARRYAPSST AQEKNPTSEY AGPNAIEAPF IFRHGGYYYL
FVSWDYCCRG TQSSYRVAVG RSKTIEGPYI DESGRDMLQG GGTVLVEGDK KTFEALGHCA
AYHFADGDLF ICHGYSVEKQ GTSVLVQRRM EWKILADGTD WPVLVP
//
