ID E6MGS5_9FIRM Unreviewed; 3225 AA.
AC E6MGS5;
DT 08-MAR-2011, integrated into UniProtKB/TrEMBL.
DT 08-MAR-2011, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE SubName: Full=AMP-binding enzyme {ECO:0000313|EMBL:EFV01815.1};
GN ORFNames=HMP0721_1208 {ECO:0000313|EMBL:EFV01815.1};
OS Pseudoramibacter alactolyticus ATCC 23263.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Pseudoramibacter.
OX NCBI_TaxID=887929 {ECO:0000313|EMBL:EFV01815.1, ECO:0000313|Proteomes:UP000004754};
RN [1] {ECO:0000313|EMBL:EFV01815.1, ECO:0000313|Proteomes:UP000004754}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23263 {ECO:0000313|EMBL:EFV01815.1,
RC ECO:0000313|Proteomes:UP000004754};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFV01815.1}.
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DR EMBL; AEQN01000016; EFV01815.1; -; Genomic_DNA.
DR RefSeq; WP_006598632.1; NZ_GL622359.1.
DR STRING; 887929.HMP0721_1208; -.
DR eggNOG; COG1020; Bacteria.
DR HOGENOM; CLU_225397_0_0_9; -.
DR OrthoDB; 51171at2; -.
DR Proteomes; UP000004754; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0009403; P:toxin biosynthetic process; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 2.
DR CDD; cd19535; Cyc_NRPS; 2.
DR Gene3D; 3.30.300.30; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 2.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 2.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR013120; Far_NAD-bd.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR PANTHER; PTHR45527:SF10; PHENYLOXAZOLINE SYNTHASE MBTB; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF00668; Condensation; 2.
DR Pfam; PF08242; Methyltransf_12; 2.
DR Pfam; PF07993; NAD_binding_4; 1.
DR Pfam; PF00550; PP-binding; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 4.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000004754}.
FT DOMAIN 1334..1409
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 3225 AA; 363478 MW; E581ECAC88DBB63D CRC64;
MNNRLSFPMT NAQLAYYMGR SQRYALGDTA AHVYYEFKNN LDADLLERAL NFAIRRHAML
RTVFHDNGMQ QILEETAPYH IQRYDLSASD DAAWETFVSR KRAVLSHEKM DPEQWPLFRV
EAAKTPDGST YLFFSFDLLI ADGSSLILVF RSIMDYYDAI ERGELPSVQP DLRFAEYAMW
QNEIKQSEKY RRDRKYWESR AEDLCFAPDL LHTEAVPKKK QYKRLIHQIL PKQWEIIKQQ
IRKNNEILTS VLFELYGEVL AYWSGDERFT IALTMSNRGA KRGRYLDVVG DFTSLMLLPY
ENTAQTNIYS RAHQVTKELM AAYRHSGCDG FEVERIISRQ RKQQHENHFD IVFTSMLDES
DANGMPMRIG RLMNSISQTP QVRLDCQLLE IENKLEITWD YLDGYYREEN MTLAFSQYIK
MIERLAVSGQ RLVLSPDAKT AEAVRAYNAT KQPELPHVSM IPIIENALKK FANKTAFKDS
RRSITFQELD IYSEVVKNAI LAEYPAGSHI AIKGNRSIST VIYIVGVIRA GCVYIPIHPD
YPADRVSYIC DACACGLYFD CDFFDGKLYG APVSAHEGTA PVAEDSSAYI IYTSGSTGLP
KGVEITHGQL CNTIFDMNAR LGLESDDVLL NVSAFSFDLS VFDVFSGITS GACVYIADDA
RDIRTLKSVL HAEQVTILNG VPSVVQLITE QSEEETYRSL RYVLMSGDFI PLTLPEALAK
ICPNAELLSL GGATEATIWS IAYPIDASVR YSESIPYGYP LANQTCYVLD KNLQLCPAGV
KGTIFIGGRG VAKGYHRDDE RTRAAFIQHH QYGRLYNTGD LGVFTSEGFI RILGRKDGQV
KVNGFRVETG EIEKTLMDSD KVNSAAVIAS GAALHKQLIG YIVPHVQNHT ALPEEAGQTL
QLLCDRCREV EAPDVAEFNQ FAALAEKTAT AHMKRIILQL CKESGLGDDF FTPELLVRRS
AVKPGYTKLM RQWLKNLAED GSIDADHDCY CVARLQSVSD AEMDALIREA ASVAGNPAFF
LNTIKYGIDI LKGEKEALTY LFPDGDFGAA EAIYRGNDIS RYFNELAAEA VFELVKSMLS
RSDGAVRILE IGAGVGGTTQ RIVELLAGLP EERIAYTFTD LSQYFLSEAK KHFSSYPFMQ
YRLFDIDKAI PEQRFGYESF DVVIAANVMH DARDVDHAMT LTTQLLAPGG VMVMLETTTD
SRIQMVSTHM IEGYNHYEDF RVAACAPLIA ADEWEQVMKR SGYAQTAAFP QRDAAASVFG
EHLILGMRGS KRNFLTETEL AQLKEDINGK LPPYMLPAHL IQLGELPLTV NGKVNKSMLP
AASTHAKGQN EHKHPKTQTE QRIFSVWQEM LGTENFGITD NLFAIGGDSI MMVRMTAILE
KQYGYKLSLN DFLTHATVKA LAELIDSSDS ADTAQSYAHD EANAYAPFPL TSVQLAYLVG
GSQNQGMESI TTQAYYELKT SWDIDQLQRA VNQVIKQQPM LHAVVSENGT QRILADYPEF
VIRRHQYHSD DDVVAIRKRL EGRPIDPHRY PMFEMESMAL PDGDHYLFLH FNLLIADGMS
MQIMIRQLYD AYYGNKMCVD HDAFTFRDYV CCRSNLLKEG IDREDAEYWR NIMPDLAPAP
PIADKPETEI INPHFRRLRT LIPKTKWQWI LSQIAENQTT AASYLLTMYS KVLGYWSGTQ
AMTINVTTFD RMPLSDNVNR IIGDFTTLVL VPVVLEKASL WDQVRRVQRQ ILQSIGHARY
DGIRVMRDYR ERVADPEKAK MPVVFTSMLF SDHGKSFEKT PGKVCYSVSQ TPQVHLDCQV
MENAEGLSVT WDFVDELFEP EKIKSMFAQF EALICSGADG VPDDGILQPL PEFIEAIDQY
NSTESATGAL TLKALIEAGL KQAPDLVAIS DDTGSMTYRE LDERTGQLAA KLQRCGAGPG
ALVAVECRKD LETVVRLLAV ARSGAAYVPL DQDYPMQRKQ TIIDQSGCRL IMRAGTDDIV
AVADKAGQEA SEDLAYVIYT SGSTGTPKGV EISQSAIINT LLDMRERMEL TSRDVFAVIT
SFCFDLSVFD LFESILLHAH LVVILDSRNI RQTARMLTEQ GVTVINCVPA TAELIVDHMT
EQNHTVRTVL LSGDFIPPGL PEKIMKKMPN AKVWSLGGAT EAAVWSIAYP ITRDYDRSQH
IPYGYPLTNQ TCYVLDEMMR LCPQGVTGEI CIGGRGVALG YRNAPELTKT AFRMHPRFGR
IYRTGDLGKA DADGCIHILG RKDHQIKING FRVETGEIEH VLEESRRIER AVVVYDKGQN
SLTGYIKPAV RPVNAPEIWR AESLFDEMRM ASNDRIRALM TGSDEAHMRR ANQSVINQMK
QVIYELIAEG RVAQPFTLAS FFDQTGVRKI YEKLMRQWFA ILIREGFMDC DGQRYSLRYD
ASSLKDWRVG LGAVQTAIGD ITFISQCREN LKEILLGKVD VLQLLFPDGK MAAAEELYKS
NQISAHYNKL TADVIGRYVG AVGEPASVME MGAGTGATSA GVIEKIQESC REYLFTDVSD
FFTDAAREKF KTYSFMKYQN YDINRSPCEQ GYPYEQHDVI LAANVMHDAV DVKKSLEYAA
SMIKPGGVLV LQEMTRTSLI QLVSTRMIEG YSDFNDFRLE KNSPILDAKE WASVLKAAGF
ETVFCFPDAK IESDLFGEHI IFAGKPAVSC YLNKAQLQDE TKRIRDKLPA YMMPSAWMQL
RAFPLTYNQK VDLKALPQPS QPEKKSEAFA QPETQEEKFL WELWRRQLGH THFGVTDDFF
LVGGDSLKAI KTIAEINERY SLEMTAFFES PDIRHIAAKM QSDGRVIREK LSALYGRMNG
QAVTPPVDAA DEWAQYEKKI SEDMAHLKLK NAAGSNENIL LFGATGYLGC YLLRSLLKNV
PRDITVIVRS ANDAAAAKRV AEGYRFYFGD AQSKAAAQRI HAVSGDFTQP RFGLKPERYD
KLANTITCVI NTAAMVKHQG VYEAFYQTNV RSVANIIQFA STGIPKTVHH VSSNGIMFGA
YPDGDNRMLF TEYDCDRGQT FTNYYLRSKF EAEKIIMQAR QTQGMNINIY RAGSIMFDSS
SGHFQRNMAE NTAYMFFRAF KKMGIAPADL DFSFDITFVD QLADAMTRII LSGVSLNDVY
HMVNPNRANL SEVFRHGLVA KNLRPMNFSE VCEFFERHFE DETYRPYIQD VLYNCEMIAL
LNRHAKQLTT RRTERLLEKL GFNWRPVSDE MIARAMHYAE KAAYI
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