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Database: UniProt
Entry: E8LFI0_9FIRM
LinkDB: E8LFI0_9FIRM
Original site: E8LFI0_9FIRM 
ID   E8LFI0_9FIRM            Unreviewed;       268 AA.
AC   E8LFI0;
DT   05-APR-2011, integrated into UniProtKB/TrEMBL.
DT   05-APR-2011, sequence version 1.
DT   28-FEB-2018, entry version 27.
DE   RecName: Full=Nitrogenase {ECO:0000256|SAAS:SAAS00692419};
DE            EC=1.18.6.1 {ECO:0000256|SAAS:SAAS00692419};
GN   ORFNames=HMPREF9443_01621 {ECO:0000313|EMBL:EFY04384.1};
OS   Phascolarctobacterium succinatutens YIT 12067.
OC   Bacteria; Firmicutes; Negativicutes; Acidaminococcales;
OC   Acidaminococcaceae; Phascolarctobacterium.
OX   NCBI_TaxID=626939 {ECO:0000313|EMBL:EFY04384.1, ECO:0000313|Proteomes:UP000004923};
RN   [1] {ECO:0000313|EMBL:EFY04384.1, ECO:0000313|Proteomes:UP000004923}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YIT 12067 {ECO:0000313|EMBL:EFY04384.1,
RC   ECO:0000313|Proteomes:UP000004923};
RA   Weinstock G., Sodergren E., Clifton S., Fulton L., Fulton B.,
RA   Courtney L., Fronick C., Harrison M., Strong C., Farmer C.,
RA   Delahaunty K., Markovic C., Hall O., Minx P., Tomlinson C.,
RA   Mitreva M., Hou S., Chen J., Wollam A., Pepin K.H., Johnson M.,
RA   Bhonagiri V., Zhang X., Suruliraj S., Warren W., Chinwalla A.,
RA   Mardis E.R., Wilson R.K.;
RL   Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: 8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP
CC       + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16
CC       phosphate. {ECO:0000256|SAAS:SAAS00692418}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|SAAS:SAAS00700914};
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|SAAS:SAAS00700909}.
CC   -!- PTM: The reversible ADP-ribosylation of Arg inactivates the
CC       nitrogenase reductase and regulates nitrogenase activity.
CC       {ECO:0000256|PIRSR:PIRSR605977-50}.
CC   -!- SIMILARITY: Belongs to the NifH/BchL/ChlL family.
CC       {ECO:0000256|RuleBase:RU003688, ECO:0000256|SAAS:SAAS00700907}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:EFY04384.1}.
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DR   EMBL; AEVN01000083; EFY04384.1; -; Genomic_DNA.
DR   RefSeq; WP_009145970.1; NZ_GL830916.1.
DR   STRING; 626939.HMPREF9443_01621; -.
DR   EnsemblBacteria; EFY04384; EFY04384; HMPREF9443_01621.
DR   eggNOG; ENOG4105DSM; Bacteria.
DR   eggNOG; COG1348; LUCA.
DR   OrthoDB; POG091H0230; -.
DR   Proteomes; UP000004923; Unassembled WGS sequence.
DR   GO; GO:0016612; C:molybdenum-iron nitrogenase complex; IEA:InterPro.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd02040; NifH; 1.
DR   InterPro; IPR030655; NifH/chlL_CS.
DR   InterPro; IPR000392; NifH/frxC.
DR   InterPro; IPR005977; Nitrogenase_Fe_NifH.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR42864; PTHR42864; 1.
DR   Pfam; PF00142; Fer4_NifH; 1.
DR   PIRSF; PIRSF000363; Nitrogenase_iron; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00746; NIFH_FRXC_1; 1.
DR   PROSITE; PS00692; NIFH_FRXC_2; 1.
DR   PROSITE; PS51026; NIFH_FRXC_3; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|RuleBase:RU003688, ECO:0000256|SAAS:SAAS00700918};
KW   ADP-ribosylation {ECO:0000256|PIRSR:PIRSR605977-50};
KW   ATP-binding {ECO:0000256|RuleBase:RU003688,
KW   ECO:0000256|SAAS:SAAS00700908};
KW   Complete proteome {ECO:0000313|Proteomes:UP000004923};
KW   Iron {ECO:0000256|RuleBase:RU003688, ECO:0000256|SAAS:SAAS00700910};
KW   Iron-sulfur {ECO:0000256|RuleBase:RU003688,
KW   ECO:0000256|SAAS:SAAS00700920};
KW   Metal-binding {ECO:0000256|RuleBase:RU003688,
KW   ECO:0000256|SAAS:SAAS00700915};
KW   Nitrogen fixation {ECO:0000256|SAAS:SAAS00692421};
KW   Nucleotide-binding {ECO:0000256|RuleBase:RU003688,
KW   ECO:0000256|SAAS:SAAS00700911};
KW   Oxidoreductase {ECO:0000256|RuleBase:RU003688,
KW   ECO:0000256|SAAS:SAAS00700919};
KW   Reference proteome {ECO:0000313|Proteomes:UP000004923}.
FT   MOD_RES      96     96       ADP-ribosylarginine; by dinitrogenase
FT                                reductase ADP-ribosyltransferase.
FT                                {ECO:0000256|PIRSR:PIRSR605977-50}.
SQ   SEQUENCE   268 AA;  29034 MW;  70E93DBE87209852 CRC64;
     MKKIAIYGKG GIGKSTTTSN LSAAFSEDGL KVCQIGCDPK NDSTRLLLGR ICSQTVLDMV
     RDNDEVAAED IVHTGYNGIK CVEAGGPEPG VGCAGRGIIV ALEKLRSLEV LTDEDLIMYD
     VLGDVVCGGF AVPIREGYAT DIYIVSSGEL MSLYAANNIA KGVKRFAMRG GVRLGGIIGN
     GRNTPNEQQL LQEFAKRLHT QLIAFIPRDV IVNKAENNRQ TVLQYAPESA QAGVYRKLAH
     DIWHNENMAI PTPMSFEELE KLVAEYGN
//
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