ID E8R1L2_ISOPI Unreviewed; 841 AA.
AC E8R1L2;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN OrderedLocusNames=Isop_2865 {ECO:0000313|EMBL:ADV63430.1};
OS Isosphaera pallida (strain ATCC 43644 / DSM 9630 / IS1B).
OC Bacteria; Planctomycetota; Planctomycetia; Isosphaerales; Isosphaeraceae;
OC Isosphaera.
OX NCBI_TaxID=575540 {ECO:0000313|EMBL:ADV63430.1, ECO:0000313|Proteomes:UP000008631};
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ATCC 43644;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Saunders E., Brettin T.,
RA Detter J.C., Han C., Tapia R., Land M., Hauser L., Markowitz V.,
RA Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Eisen J.A.;
RT "The complete sequence of chromosome of Isophaera pallida ATCC 43644.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADV63430.1, ECO:0000313|Proteomes:UP000008631}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43644 / DSM 9630 / IS1B
RC {ECO:0000313|Proteomes:UP000008631};
RX PubMed=21475588; DOI=10.4056/sigs.1533840;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Goker M., Cleland D., Saunders E., Lapidus A., Nolan M., Lucas S.,
RA Hammon N., Deshpande S., Cheng J.F., Tapia R., Han C., Goodwin L.,
RA Pitluck S., Liolios K., Pagani I., Ivanova N., Mavromatis K., Pati A.,
RA Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D.,
RA Detter J.C., Beck B., Woyke T., Bristow J., Eisen J.A., Markowitz V.,
RA Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Isosphaera pallida type strain (IS1B).";
RL Stand. Genomic Sci. 4:63-71(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP002353; ADV63430.1; -; Genomic_DNA.
DR AlphaFoldDB; E8R1L2; -.
DR STRING; 575540.Isop_2865; -.
DR KEGG; ipa:Isop_2865; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_5_0; -.
DR InParanoid; E8R1L2; -.
DR Proteomes; UP000008631; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000008631}.
FT DOMAIN 23..299
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 300..574
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 677..739
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 751..775
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 44..51
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 841 AA; 92431 MW; F0D1A3B01F2BE388 CRC64;
MTNPPPPRNV KVADPHALDP LMADLTPAQR QAVAHLEGPM LVLAGAGSGK TRVITRRIAY
LIRSGAVGSS ILAITFTNKA AGEMKRRIED IVPNSGVWVG TFHSVCARLM RTYAPLIGLD
RGFTIHDSSD RIKVIKACVR QLGLDDAGVS AERLEAVISK AKNDLVAPET LARNAWNHEA
VLAARVYPLY QQRLREQSAV DFDDLLLHMV AILKNHPEVR KTLDARFRFL LVDEYQDTNL
AQYAILKALS VDHPNLCVTG DPDQSIYAWR GANIHNILEF EKDFPGCKVV KLERNYRSTR
NILTTADGLI RHNRLRKPKA LLTENPPGAP VRVVTHANEA AEARAVAAAI RDRVDHHGFS
YSDIAIFCRV TALTRNLESA LRSARIPYQV VGGVAFYERQ EVKDLLAYLK LAVNPKDDVA
FERVVNTPPR GLGQTTLDHL RAYASRVGIP LLRAAREAQQ VAAIKDKAAR SLRDFALLMD
ELATLRDQPA EAALRSVLAL TSYRAYLAEA PQGEAEERLA NLDELVSAAR EYDATHTEPT
LDGFLEEVSL ISAVDRMRDD AGAVAVMTLH AAKGLEFPVV FIIGLEEGIL PHSRSSESRE
ELEEERRLLF VGITRAQKEL TISHCKIREY RGQRQVMIPS CFLNELPEES VEYEDRSARE
PSGFHWVEGN HRAFQSSGRN GASWGLVPRE PNHPAGRPRT MRSPTHDDDA IDPPAGDDLP
EVPIGRPAAS SGSSSSQSTG FRMITAADLA GAHPTHSSQD EPGQSPDPIQ KLGPQDVERL
KPGTAVLHPH YGLGRVTGID GAGPQRKGRI RFAAGEKTFV LAKTPLRIIS KPDGLSHLQG
T
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