ID E9AUG6_LEIMU Unreviewed; 4822 AA.
AC E9AUG6;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 71.
DE RecName: Full=Midasin {ECO:0000256|PIRNR:PIRNR010340};
GN ORFNames=LMXM_20_0260 {ECO:0000313|EMBL:CBZ26594.1};
OS Leishmania mexicana (strain MHOM/GT/2001/U1103).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania.
OX NCBI_TaxID=929439 {ECO:0000313|EMBL:CBZ26594.1, ECO:0000313|Proteomes:UP000007259};
RN [1] {ECO:0000313|EMBL:CBZ26594.1, ECO:0000313|Proteomes:UP000007259}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MHOM/GT/2001/U1103 {ECO:0000313|EMBL:CBZ26594.1,
RC ECO:0000313|Proteomes:UP000007259};
RX PubMed=22038252; DOI=10.1101/gr.122945.111;
RA Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A.,
RA Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D.,
RA Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F.,
RA Hertz-Fowler C., Mottram J.C.;
RT "Chromosome and gene copy number variation allow major structural change
RT between species and strains of Leishmania.";
RL Genome Res. 21:2129-2142(2011).
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|PIRNR:PIRNR010340}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FR799573; CBZ26594.1; -; Genomic_DNA.
DR RefSeq; XP_003875090.1; XM_003875041.1.
DR GeneID; 13448553; -.
DR KEGG; lmi:LMXM_20_0260; -.
DR VEuPathDB; TriTrypDB:LmxM.20.0260; -.
DR OMA; ILEQWHR; -.
DR OrthoDB; 1221059at2759; -.
DR PhylomeDB; E9AUG6; -.
DR Proteomes; UP000007259; Chromosome 20.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 6.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 7.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR PIRSF; PIRSF010340; Midasin; 3.
DR SMART; SM00382; AAA; 6.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340}.
FT DOMAIN 22..164
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 350..589
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 755..900
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1051..1206
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1370..1585
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1723..1872
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 3400..3430
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3805..3832
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3948..3979
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3994..4482
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4001..4019
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4020..4034
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4035..4051
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4052..4070
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4071..4094
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4095..4127
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4136..4165
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4194..4223
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4228..4264
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4272..4286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4329..4349
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4350..4364
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4379..4398
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4409..4456
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4822 AA; 534708 MW; 62DD0FF012331406 CRC64;
MAMILTPTVA RNLESIQLAL ETSNAVLIQG EIGCGKSASV LHLAEKYAAK ETMIQVNVDD
TFDSKDLLGK FSATQTPGAF QWVPGPLTVA VQKGYWVLME DIDLASFDVF AVLLSLLESS
TLFISDKNKF LRAHSGFRLI ATQQLYSSGA AVLTRKSNSI PYAELWGTVI MENLTAEEMC
TIVNHTYNNV PLSIIQSLAT LASPTSGARL VGLQNLLRWS KRVSKRLPNT PCIDNANSKT
TPAFISSRTR ETMVKEAFDC LVAKYPPGNE RAGVLKMISE ACGVPINVAE PLVLECRPQE
SLVTDMYSVG RVCIPLISTV SGLLRDSRIA FAPTTQAMSL LERLAAAVES HEHVLLTGET
GVGKTFIVQY LADQLGQSLI VHNLNQQTDS SDFMGGWKPL DVGVTVRDLH NGFTELFAAC
FNAEKNAQFL NILQQFVNGR KWGRALTQII KGCGSFRAKS AKVEYDSAVQ QQWEDLEQKA
NELLDTVEKT RTNFAFYFEE GSLVKAWREG HWILLDELNL ASTEVLERVS SVLGDSDRLF
LTDKDSSEPI MRHKNFHVFA NMNPPTDVGK KDLPPSLRSR FVEYYVSEPF NRNDISIVVR
AYIGFLTADA KVDEITDFFL DCVSNAKTKL CTLDGESKAP SFSLRTLTRA LSYVRRATPL
YGFAVALFDG LLLGFATPLQ RQFHAVVEQL IVKNIFRGKR PNSPTLPAAS KEGSFIAYEH
IWIPAGTEAP YCDEAFILTN SVKGHLKNLS RAVFANRPVL LEGPTSSGKS SMVKYLAELT
GNKFVRINNH DSTEVQEYLG HYVTDELGKL RYVDGILVKA VRHGYWVVLD ELNLAPTDVL
EALNRLLDDN CELFVADTQE TIKPHPQLRI FATQNPAGIY SGRKMLSRAF RNRFLEITVD
EIPTAEISTI LCRRYSLPQS FADKMVEVMT ALQIHRQSSQ IFAGRHGFIT PRDLFRWAER
RPETYQQLAE HGFLLLGERC RKPSERQVVR DVIESVTKTE LDDKTIYHPS HWPFVQHYFS
QVESGVIDEF KIVWTESMQR LFTIVGICLE HKEPVLLVGE TGSSKTTVCQ LWAALLGIPL
HILNCHQHTE ASDFLGSLRP SPPDRRDHAL FEWRNGPLVQ SMLNGDMFVL DEISLTEDSV
LERLNSVLEP SRSVTLAEKS SADCIMAHER FLVMATMNPG GDFGKKELSP ALRNRFTEIY
VRSTTDIAEV SMILSHRLEA ELAVWAPHMA AVLCRVSQST TTSSSQQHIS IRDIISWVTF
MNAAHGRCPP EVAFLQGLDA VILDGIGVGT GQSEASCAVL KSSCTSQAVQ EVMKSGHAPI
DHKALLAKPF WEVCEGMAEP HLPPSMQGRF FFGAPATRRN LSKLMRASLV KKAVLLEGSP
GVGKTSIVEA LGCALNRSVV RINLSEQTDI MDLFGTYLPS PSDAAEQEEA AAEGEVSAAT
AGNELERQQP CGGRGSVKGP QFAWSDGVLL RALKEGSWVI LDELNLASQS VLEGLNALLD
HRSTVFIPEL NEEFHAHIDF RVFACQNPLL EGGGRKGLPC SFINRFIKVH VEAFEVSDLI
VIAQAVCPDI EETTLRRMVD FVDRLQHDIM VRRSYGLRGS PWEFNLRDVL RWAKLLQSYE
ARKRPTSFAD MLFLQRMRTD EDAARCRLAL ESCFSKEEVE RDVGHGCDSD FLIHDNAVYF
AKQRLMQRET AAISASDAQQ LSSLLLLPSQ AHLVKSLLVC VEQNQFALLV GPSGAGKTFA
LRTAAVLAGA KLVTFSMTAS CDTVDLLGGF DQVEGKQGEF EWRDSLVLEA MLQGHWLVLD
NVNYCNASVL DRLNPLVEPN GVLFVNEQGI VNNEVRVIRP HPRFRLFATL NPRYGEISRA
MRNRAVELYV KPVAIPSAES FALSAHASVV AQERHRLSQD AKSADEAANF VASLSAPPVR
LLWRLLRYHQ TFVSVGFHAD SQRTGTALLS DALSAGAPSV FTLLRTSCLL RPSSLIADLE
RLILHRYIHN RTGHWASFKP ELLELLRRIM DGDMEENDTA AFADEAMEVL CALRLDGRDD
EMFDRAAATE DEEAATASDP VARALVCRRV GRSALALQRL LSESASATEH DIKDAAEAAK
QNEKRTLANR HRLEDLVLWF VLEQCSDSVP LHAVTETRRL WISHRLPAEL AQRVLSVVRT
DFCSPEMLAG LYTRAVLLLG PHTDALTALW GSQMQVASST LVNNALVHLC GVLQAAFASH
NATPVPPATF QQLLRLAAPF ARLVVQHANE EYGVHTDNYI PLVTYTALVC KAASKMNPSM
PHAMTLAHAA TSFLDSLQQH LGLPFPLLGG KAPLHRYVPT PHITRAEERR LLQAWNTAAN
AASAARPTAQ SNSAVEPRAL LHRLWESAAF REGFACLQQL LYIRATAATY ASVTLADPAH
TVHPLLPALQ TRMTAFVDTY TARLPLFTEL ELALWRGVHQ LSLDKPTDTT EALGKLTPLL
LSRFASRHGV AGTRHLFAGA WAVAAADCMR YGAQVPVRDI GLADNPRLQA LTGLATFLQT
CPLAAASPLE EWATNSQREL VGVLCACFPD LASCVPTPLP PSRTSAAALT RLSVFGQAET
VVASAAPMPS LVLERQRVLM AALLHRLRSN GCDGADTLSA VTFLWVLKCR LLLPQSPVDP
IYRQECKRVV ATEELELLDR LDGAFAWAEQ LAIRHHSPQR EILLTLRVQE KELLRQAQRK
YVTRPDATGM KYATLIRQLY QVCTTLLSDA RVLGLFEMPP ASAGGLNAVA AAEIIASREA
WSDLLYEQVM QLLAEYGGYE DVTVNFAEAA LLASMAARAS CATDFRLLAA ADSSALSRTL
FSRADTLRLL QFPSPLVHAL PMPPLQHTAS LLHHRVVYLD SCLSLLSGTA PQGTSRDFVE
DVFAQYRTLF HQIEEWEAQE RQANQLAVMY KEKATVIEGD DEKLIRKLKE LFPSYEAEFR
IDTLEDDSAD PSDRKKTEEE LEGRRAKFAR ILFKERNGFY LRHLVEVHRT FYWRLISCRM
VEAATASSAC SATSPQSAAL LAAFEKRFDA LAAELHTFPA SSGSTSGVAG MEEQLLLNGF
AARVSLLREE MRGTAVSLEE RRVAAFNIFL DTDVYELSRF SAPLQTLLDA VQRLAERYPD
TPSLQRCLRI GHKIAALPAM STPLMKVMAG CEVLLRECYE WERNASHEVS LMRHMTELSS
FVLRWRRLEL HCWAHVFQAK RAELELQAAQ QWFTLYDLLL GEAVTCESDG EDHDTVAVWD
AETPLLRRCR AAFQRASKFM WDAIFGDFSV RLRILQGFGF QLLAAGSAQA PLANTVLHVC
DFFAQFEPLV QQQRDKAVQP IEEDIAEFAK IMRWEDANYY AVRATAEKSH MKMARVLGAL
EEVLRTPVLA VITAEEQRCE DDASLGFALP LKVDSKKVKQ ARKPAEAAGK GAKQSIKRQR
NGKAVSAAAD NPPLQTVATA EDPALQLLSR NVYLNQVHTF LRDAAHEVIE NVTALQKPKT
PNQMKVRALK TLFGRLSEAG VPHTHENQVG DWGVLFFTTE ALLGCRETHH DQTAAVARVG
AAAAEQYRFA RWVQRLREVE RKPHHDLSGA QAKRGTGIAE SLFANAVHAA TVLCELFHLY
EQLRVLEVAV QHSALASDGD TDAGVVSHAV PRVFLDDAVE VACLVQSVCS GLGWMVQQPH
MVAKTTAEAH GLVHELAGVS NELWSLCTSQ EPLRRSAAST VPPGISKHYF DVLHRVLGVV
CRIPACDVAD IAAAAKPLVD AVTDILARHE QPSVQIHERL AEEAKRRRTE QLPKPWAQRL
RHAIEEVSDA LVPCGEGASV VLEDDGRMPS AAATADAEAK ASGHSDSESD ADAPKCESAV
YARYTIHGAA VHEALQRLVP VLEDLLASPA SSEGATAATV VTSSALPSVC TLAAREREEL
LPRLSVCRRE VQRARDAWCS VLEAQCSFGV VVARLFTVLL KKGFCKTEDE EPEDGEGDGE
GGQQQNGTGM DDGQGEKDVT DQIDNEDQLM NMKDKEEQQG DEQRQDEEEE GEEDTAADVE
TDFNGQKEHR SESDGEGDEE GEESDKEMGD VDDGTEQERK KTKKDAGDAD DMNEDAGDAA
EEVPEDDLAE GDEHREDNEE TGGFDDKADE VRDADEDRSC EHDMVGDDDK SEMDEDGGSG
GDSVEHSDTD GEEHEESARD DKLPSDADEE PEESDVASED RVDSDAATDA ERSEFGEEEV
EQQDNSENAG DASDIEDASD GADENVYDGG QQDDREGDEA TENQDKDRKD TSAKVRKEQA
ANEEEQPGTE QEQDDAGQNW KQQQEQNEQA QRRDNAEQTR SQHNPYRAVK EALQRHQRQM
QQLNLNQHVE VTDEKDEPKA EKPPKTDQAD DPEDVEEFDF DEDGDREGIA AAEQTEAKPT
ETDAYEGEES HSDASSDEDG VGEDVDESAD APQDTRRKRR QNKDELTELE EREAAEATSR
KKRSKEKVRT TAGDEDASAS EDQEDDDSTK KDDQDAAAEA NAKLERGRQL WLEQETAVQG
LSQQLCEQLR LILTPTLADK LQGDYKTGKR LNMKRIIPYI ASQFKKDRIW LRRTKPSRRT
YQILVALDDS LSMQCNNAGV LSCRAVALIA KALQQLEVGE FGVACFGKET HVVHPLEEPF
LADSGPRLFS EITFEQKSTN MKLFLETTLG YLDDARDRMR GQTRSTTQQL QQIMFIIGDG
QITENRDDLR QLMVRAEENH QMVVFVLLDI KAAEVDTATP SAGPSAAPAA AAPLRAKALQ
GLSTAERLRR LKADREARLQ RVQSSSILDM QVVEFRAGRV VRRPYMEDFP FPYYLIIRDI
QTLPEIIADA MRQWFELLNV CA
//
