ID E9GC29_DAPPU Unreviewed; 704 AA.
AC E9GC29;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=acylaminoacyl-peptidase {ECO:0000256|ARBA:ARBA00012917};
DE EC=3.4.19.1 {ECO:0000256|ARBA:ARBA00012917};
GN ORFNames=DAPPUDRAFT_302098 {ECO:0000313|EMBL:EFX83217.1};
OS Daphnia pulex (Water flea).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
OC Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
OX NCBI_TaxID=6669 {ECO:0000313|EMBL:EFX83217.1, ECO:0000313|Proteomes:UP000000305};
RN [1] {ECO:0000313|EMBL:EFX83217.1, ECO:0000313|Proteomes:UP000000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21292972; DOI=10.1126/science.1197761;
RA Colbourne J.K., Pfrender M.E., Gilbert D., Thomas W.K., Tucker A.,
RA Oakley T.H., Tokishita S., Aerts A., Arnold G.J., Basu M.K., Bauer D.J.,
RA Caceres C.E., Carmel L., Casola C., Choi J.H., Detter J.C., Dong Q.,
RA Dusheyko S., Eads B.D., Frohlich T., Geiler-Samerotte K.A., Gerlach D.,
RA Hatcher P., Jogdeo S., Krijgsveld J., Kriventseva E.V., Kultz D.,
RA Laforsch C., Lindquist E., Lopez J., Manak J.R., Muller J., Pangilinan J.,
RA Patwardhan R.P., Pitluck S., Pritham E.J., Rechtsteiner A., Rho M.,
RA Rogozin I.B., Sakarya O., Salamov A., Schaack S., Shapiro H., Shiga Y.,
RA Skalitzky C., Smith Z., Souvorov A., Sung W., Tang Z., Tsuchiya D., Tu H.,
RA Vos H., Wang M., Wolf Y.I., Yamagata H., Yamada T., Ye Y., Shaw J.R.,
RA Andrews J., Crease T.J., Tang H., Lucas S.M., Robertson H.M., Bork P.,
RA Koonin E.V., Zdobnov E.M., Grigoriev I.V., Lynch M., Boore J.L.;
RT "The ecoresponsive genome of Daphnia pulex.";
RL Science 331:555-561(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cleavage of an N-acetyl or N-formyl amino acid from the N-
CC terminus of a polypeptide.; EC=3.4.19.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000721};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; GL732538; EFX83217.1; -; Genomic_DNA.
DR AlphaFoldDB; E9GC29; -.
DR STRING; 6669.E9GC29; -.
DR EnsemblMetazoa; EFX83217; EFX83217; DAPPUDRAFT_302098.
DR KEGG; dpx:DAPPUDRAFT_302098; -.
DR eggNOG; KOG2100; Eukaryota.
DR HOGENOM; CLU_014230_1_1_1; -.
DR InParanoid; E9GC29; -.
DR OMA; QEIATPF; -.
DR PhylomeDB; E9GC29; -.
DR Proteomes; UP000000305; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR045550; AARE_N.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001375; Peptidase_S9.
DR PANTHER; PTHR42776:SF4; ACYLAMINO-ACID-RELEASING ENZYME; 1.
DR PANTHER; PTHR42776; SERINE PEPTIDASE S9 FAMILY MEMBER; 1.
DR Pfam; PF19283; APEH_N; 1.
DR Pfam; PF00326; Peptidase_S9; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF50993; Peptidase/esterase 'gauge' domain; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000000305}.
FT DOMAIN 34..270
FT /note="Acylamino-acid-releasing enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF19283"
FT DOMAIN 488..701
FT /note="Peptidase S9 prolyl oligopeptidase catalytic"
FT /evidence="ECO:0000259|Pfam:PF00326"
SQ SEQUENCE 704 AA; 79140 MW; C30F5FB9EB99804C CRC64;
MALKYTLEEA VTAWRRTSQI VFYNAGRLNI QNDVIDVAVT ASQRNLAKEE SITFQTHYIA
QKSNFNLLSQ TPSSDVSTEK LKAFGKTSNV TAIIREIPDS KSAEKKNRQL LEIWKDNSLH
SSVDLQLFDK HGKVYTDGTF GCLEFSPDEK HLVYLAEKKE PKKQSFLQFV MAATVEGSKV
GVEYDFVEDW GEQLVGKSQP VICIFKVDWE PFQSEDCVRI LEASKEWSPG QLIWCSNNQL
AGVAWFHQPR RLGIIYCSNR PSQIFKVDIS SGKYDWFGLK TNTAASPRHH SESDTVIYLS
SLAYGPHHKE QKISSISSDG TVRQVETGTS ESYQGMYNQS FPDRCWSPDG KLVFFTTPCK
SSVQSYALNL NSSSVCKLSL PEGCTGSVVL DVFQDMILVC GVSLIRPDQL FIGRFNSEKI
NEEPIEWKCL TGKKSLPPTL SLASDVFSVK LAGDMEYEAS LIYPKNPSKK TPLVVAPHGG
PHSVSTDQFK AEVYFFSQLG YAVLLVNYRG STGFGEKSLY SLLGKVGEQD VQEVHNATVQ
MIEKHSEFLD KELVFLFGGS HGGFLVTHLS GQYPDFYRAV STRNPVIDMA TMFPITDIAD
WTIVESNLGD GSQLEKLLEP KTFSKMWELS PIRYVKQVKA PTLLLVGKID RRVPPTQSIE
YYRALQLHGI KTRMIMYEDC HSLSQVPVDT DALINTVMWF QQSL
//
