ID E9HDJ7_DAPPU Unreviewed; 785 AA.
AC E9HDJ7;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=[heparan sulfate]-glucosamine N-sulfotransferase {ECO:0000256|ARBA:ARBA00012979};
DE EC=2.8.2.8 {ECO:0000256|ARBA:ARBA00012979};
GN ORFNames=DAPPUDRAFT_61590 {ECO:0000313|EMBL:EFX70155.1};
OS Daphnia pulex (Water flea).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
OC Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
OX NCBI_TaxID=6669 {ECO:0000313|EMBL:EFX70155.1, ECO:0000313|Proteomes:UP000000305};
RN [1] {ECO:0000313|EMBL:EFX70155.1, ECO:0000313|Proteomes:UP000000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21292972; DOI=10.1126/science.1197761;
RA Colbourne J.K., Pfrender M.E., Gilbert D., Thomas W.K., Tucker A.,
RA Oakley T.H., Tokishita S., Aerts A., Arnold G.J., Basu M.K., Bauer D.J.,
RA Caceres C.E., Carmel L., Casola C., Choi J.H., Detter J.C., Dong Q.,
RA Dusheyko S., Eads B.D., Frohlich T., Geiler-Samerotte K.A., Gerlach D.,
RA Hatcher P., Jogdeo S., Krijgsveld J., Kriventseva E.V., Kultz D.,
RA Laforsch C., Lindquist E., Lopez J., Manak J.R., Muller J., Pangilinan J.,
RA Patwardhan R.P., Pitluck S., Pritham E.J., Rechtsteiner A., Rho M.,
RA Rogozin I.B., Sakarya O., Salamov A., Schaack S., Shapiro H., Shiga Y.,
RA Skalitzky C., Smith Z., Souvorov A., Sung W., Tang Z., Tsuchiya D., Tu H.,
RA Vos H., Wang M., Wolf Y.I., Yamagata H., Yamada T., Ye Y., Shaw J.R.,
RA Andrews J., Crease T.J., Tang H., Lucas S.M., Robertson H.M., Bork P.,
RA Koonin E.V., Zdobnov E.M., Grigoriev I.V., Lynch M., Boore J.L.;
RT "The ecoresponsive genome of Daphnia pulex.";
RL Science 331:555-561(2011).
CC -!- PATHWAY: Glycan metabolism; heparan sulfate biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005093}.
CC -!- PATHWAY: Glycan metabolism; heparin biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004841}.
CC -!- SIMILARITY: Belongs to the sulfotransferase 1 family. NDST subfamily.
CC {ECO:0000256|ARBA:ARBA00010420}.
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DR EMBL; GL732624; EFX70155.1; -; Genomic_DNA.
DR AlphaFoldDB; E9HDJ7; -.
DR STRING; 6669.E9HDJ7; -.
DR EnsemblMetazoa; EFX70155; EFX70155; DAPPUDRAFT_61590.
DR KEGG; dpx:DAPPUDRAFT_61590; -.
DR eggNOG; KOG3703; Eukaryota.
DR HOGENOM; CLU_011357_2_0_1; -.
DR InParanoid; E9HDJ7; -.
DR OMA; GRQYPTM; -.
DR PhylomeDB; E9HDJ7; -.
DR UniPathway; UPA00756; -.
DR UniPathway; UPA00862; -.
DR Proteomes; UP000000305; Unassembled WGS sequence.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0015016; F:[heparan sulfate]-glucosamine N-sulfotransferase activity; IBA:GO_Central.
DR GO; GO:0019213; F:deacetylase activity; IBA:GO_Central.
DR GO; GO:0015014; P:heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; IBA:GO_Central.
DR GO; GO:0030210; P:heparin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR021930; Heparan_SO4_deacetylase.
DR InterPro; IPR037359; NST/OST.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000863; Sulfotransferase_dom.
DR PANTHER; PTHR10605:SF56; BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE; 1.
DR PANTHER; PTHR10605; HEPARAN SULFATE SULFOTRANSFERASE; 1.
DR Pfam; PF12062; HSNSD; 1.
DR Pfam; PF00685; Sulfotransfer_1; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR637359-3};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000000305}.
FT DOMAIN 7..406
FT /note="Heparan sulphate-N-deacetylase"
FT /evidence="ECO:0000259|Pfam:PF12062"
FT DOMAIN 496..744
FT /note="Sulfotransferase"
FT /evidence="ECO:0000259|Pfam:PF00685"
FT ACT_SITE 505
FT /note="For sulfotransferase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-1"
FT BINDING 603
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT BINDING 724..728
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-2"
FT DISULFID 709..719
FT /evidence="ECO:0000256|PIRSR:PIRSR637359-3"
SQ SEQUENCE 785 AA; 90691 MW; F8E96683D213AC92 CRC64;
MYFPFSRYKL EVVGKSLPIL TNVDKGKYGV IVFENLDRYL TMDKWNRELL DKYCRQYQVG
IIGFMSPREE SLIGAQLRGF PLHIHTNFKL KDAELKADSP VLRITRAGDV LWGPLPGNDW
TVFSPNHSTY EPLAMGSIQA LEGSAGFGGL SSNVITVLRD CGEFDGIQRI FFGSGLDFWL
HRLLFLDSMS FLSHGRLSTS LQRYIQVDID DIFVGQKGTR LTPSDVESII DTQSRIQTIV
PGFRFNMGYS GKFFNHGTDE ENKGDEYLLQ RAKEFTWFGH MWSHQQPHLY ENITLLEADM
ILNKKFAIEH GIPTDSGYSV SPHHSGVYPV HEALYEMWKK LWNIHVTSTE EYPHLRPARL
RRGFIHRGIM VLPRQTCGLY THTIYIDKYP GGRSTLDSSI KGGELFQTIV FNRINIFMTH
MSNYGNDRLA LYTFEAATKF LQCWTNLQFQ SISALQLADK YFRMYPEESE PVWGNPCDDQ
RHMKIWSQNK TCDQLPRFLV LGPQKTGTTA LYTFLSMHPA IVSNYPSPET FEEIQFFNGK
NYYKGLDWYM SFFPVPKNST AKFLFEKSAT YFDGELVPRR VHALLPKAKL VIIIISPAKR
AYSWYQHMRS HGDPTALNYT FYQVLTAGDA QPKALRDIRF RCLSPGMYAH HLDRWLLYFP
PQQISVIDGE QVRLDPVTSM TKLQHFLKIR PIFDYSLHLR YDARKGFFCQ VVNGDHTKCL
GRSKGRHYPA MDAQSAKYLQ SFYMTHNIAL EKLLKRLGYP IPLWLEEDLS DRPVNNQAVE
LSNSI
//
