ID E9Q3Z4_MOUSE Unreviewed; 867 AA.
AC E9Q3Z4;
DT 05-APR-2011, integrated into UniProtKB/TrEMBL.
DT 05-APR-2011, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE RecName: Full=hexokinase {ECO:0000256|ARBA:ARBA00012324};
DE EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
GN Name=Hk3 {ECO:0000313|Ensembl:ENSMUSP00000115227.2,
GN ECO:0000313|MGI:MGI:2670962};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000115227.2, ECO:0000313|Proteomes:UP000000589};
RN [1] {ECO:0000313|Ensembl:ENSMUSP00000115227.2, ECO:0000313|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000115227.2,
RC ECO:0000313|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0007829|PubMed:21183079}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3] {ECO:0000313|Ensembl:ENSMUSP00000115227.2}
RP IDENTIFICATION.
RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000115227.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000256|ARBA:ARBA00004888}.
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000256|ARBA:ARBA00005028}.
CC -!- SIMILARITY: Belongs to the hexokinase family.
CC {ECO:0000256|ARBA:ARBA00009225}.
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DR RefSeq; NP_001193321.1; NM_001206392.1.
DR AlphaFoldDB; E9Q3Z4; -.
DR SMR; E9Q3Z4; -.
DR MaxQB; E9Q3Z4; -.
DR PeptideAtlas; E9Q3Z4; -.
DR ProteomicsDB; 369579; -.
DR Antibodypedia; 29158; 483 antibodies from 35 providers.
DR DNASU; 212032; -.
DR Ensembl; ENSMUST00000153665.8; ENSMUSP00000115227.2; ENSMUSG00000025877.15.
DR GeneID; 212032; -.
DR AGR; MGI:2670962; -.
DR CTD; 3101; -.
DR MGI; MGI:2670962; Hk3.
DR VEuPathDB; HostDB:ENSMUSG00000025877; -.
DR GeneTree; ENSGT00950000182787; -.
DR HOGENOM; CLU_014393_1_0_1; -.
DR OMA; VRPCEVG; -.
DR OrthoDB; 5481886at2759; -.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR BioGRID-ORCS; 212032; 3 hits in 80 CRISPR screens.
DR Proteomes; UP000000589; Chromosome 13.
DR Bgee; ENSMUSG00000025877; Expressed in granulocyte and 86 other cell types or tissues.
DR ExpressionAtlas; E9Q3Z4; baseline and differential.
DR Genevisible; E9Q3Z4; MM.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0004396; F:hexokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro.
DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR Gene3D; 3.30.420.40; -; 2.
DR Gene3D; 3.40.367.20; -; 2.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; HEXOKINASE; 1.
DR PANTHER; PTHR19443:SF1; HEXOKINASE-3; 1.
DR Pfam; PF00349; Hexokinase_1; 2.
DR Pfam; PF03727; Hexokinase_2; 2.
DR PRINTS; PR00475; HEXOKINASE.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR PROSITE; PS00378; HEXOKINASE_1; 2.
DR PROSITE; PS51748; HEXOKINASE_2; 2.
PE 1: Evidence at protein level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Proteomics identification {ECO:0007829|EPD:E9Q3Z4,
KW ECO:0007829|MaxQB:E9Q3Z4};
KW Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..174
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 182..415
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
FT DOMAIN 426..617
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 623..857
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
SQ SEQUENCE 867 AA; 94231 MW; 00043A686D3E52DE CRC64;
MEQALKGQDS PAPSVRMLPT YVRSTPHGTE QGDFLVLELG ATGASLRVLW VTLTGTKECR
VEPRSREFVI PQEVILGAGQ QLFDFAARCL SEFLDAYPVE NQGLKLGFNF SFPCHQTGLD
RSTLISWTKG FRCSGVEGQD VVQLLRDAIQ RQGTYRIDVV AMVNDTVGTM MGCELGTRPC
EVGLIVDTGT NACYMEEARH VAALDEDRGR TCVSIEWGSF YDEDALGPVL TTFDSALDRE
SLTPGAQRFE KMIGGLYLGE LVRLVLVHLT QHGVLFDGCA SPALLSQGCI LLDHVAEMED
TATGTARVHT ILQDLGLSPR ASDAELVQYV CVAVCTRAAQ LCAAALAAVL SRLQHSREQQ
TLQVAVATGG RVFERHPRFL RILKETVTLL APNCDVSFIP SVDGGGRGVA MVTAVAARLA
AHRRILEETL APFQLTLEQM TVVQAQMREA MIRGLQGEAS SLRMLPTYVR ATPDGSERGD
FLALDLGGTN FRVLLVRVAE GSVQIINQVY SIPECRAQGS GQKLFDHIVD CIVDFQKRQG
LSGQSLPLGF TFSFPCKQLG LDQGILLNWT KGFNASGCEG QDVVYLLREA IRRRQAVELN
VVAIVNDTVG TMMSCGYDDP RCEMGLIVGT GTNACYMEEL RNVASVPGDS GLMCINMEWG
AFGDDGSLGT LSTRFDTSVD QASINPGKQR FEKMISGMYL GEIVRHILLH LTNLGVLFRG
QKTQCLQARD IFKTKFLSEI ESDSLALRQV RAILEDLGLT LTSDDALMVL EVCQAVSRRA
AQLCGAGVAA VVEKIRENRG LQELTVSVGV DGTLYKLHPH FSKLVSATVR KLAPQCTVTF
LQSEDGSGKG AALVTAVACR LTQMAHV
//
