ID F0FCB3_STRSA Unreviewed; 985 AA.
AC F0FCB3;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 24-JAN-2024, entry version 48.
DE RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN Name=hsdR {ECO:0000313|EMBL:EGC23387.1};
GN ORFNames=HMPREF9388_0296 {ECO:0000313|EMBL:EGC23387.1};
OS Streptococcus sanguinis SK353.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=888815 {ECO:0000313|EMBL:EGC23387.1, ECO:0000313|Proteomes:UP000004185};
RN [1] {ECO:0000313|EMBL:EGC23387.1, ECO:0000313|Proteomes:UP000004185}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SK353 {ECO:0000313|EMBL:EGC23387.1,
RC ECO:0000313|Proteomes:UP000004185};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC -!- SIMILARITY: Belongs to the HsdR family.
CC {ECO:0000256|ARBA:ARBA00008598}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGC23387.1}.
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DR EMBL; AEWY01000002; EGC23387.1; -; Genomic_DNA.
DR RefSeq; WP_002897161.1; NZ_GL872307.1.
DR AlphaFoldDB; F0FCB3; -.
DR REBASE; 234148; Ssa353ORF292P.
DR PATRIC; fig|888815.3.peg.295; -.
DR HOGENOM; CLU_010804_0_0_9; -.
DR Proteomes; UP000004185; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.90.1570.50; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR010908; Longin_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR InterPro; IPR040980; SWI2_SNF2.
DR PANTHER; PTHR42927; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR42927:SF1; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF18766; SWI2_SNF2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS50859; LONGIN; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:EGC23387.1};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT DOMAIN 281..487
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 933..985
FT /note="Longin"
FT /evidence="ECO:0000259|PROSITE:PS50859"
SQ SEQUENCE 985 AA; 112755 MW; 7654615D6466AFC3 CRC64;
MANLDITERY DFQQWILKAL IQYNGYIQRA QSAYNQRLAM DIDILWEFLM STQEATVEKL
LKRFDRETIL NLINQEIIKG GLLFALKDGV YIENKKLRLM YRRPATDFNE KASHQYNLNR
LTVMEEVVYK DQERIDLVIF LNGLALFAIE LKLNSSGQSV QDAIKQLKGR DVKNRLFRFN
AGVLVSFAVD WMEVYMTTQL KGSDTYFLPF NKGMGEGIDQ GAGNPQNNQG PETEYLWRDL
LKKESILQLI ERFIFLTPDK KDIIFPRYQQ RRAVNRILED MRVNHTDRNY LIQHSAGSGK
TNTIAWLAHS LVSLHDEENQ NIVDTVLVVT DRIVVDRQLQ DAIRSIEHQP GVIKVMDDKA
TSSDLADALA GNTKIVATTI HKFAQINKSN WMSAGVTAEK KFAILIDEAH SSTTGSYMSA
VSQVLTETDI ENGTMDEPQE VSIGDAIEQE IARTGKQDNI SIIAFTATPK ATTLQLFGST
RPDGSKGPFD LYSMKQAIEE GFILDVLNNY TTYKTYYKLN KAVEEDPELK TTVAKRKIAK
FVELSDENIH QKVEIIMDHF ITHDIMADLG GHGKAMIVTS GREAAVKYQQ AFKKYLKEHH
ITSIGAAVAF SGRLSLDGAE FSESSMNGFS EDKLKSKFDT DDYQVLIVAN KYQTGFDQPK
LLAMYVDKKL RDVAAVQTLS RLNRIVKGYH KKTFILDFKN TYEDINRAFA PYYRETILTE
TITPSDVFDL DAKIESYGIV DNDDVHAYNQ FLYQEKRSSR DKQKMLALLS NGYARVQRFD
EKEQVNIRKV IRGFLRLYTF LIQATAYQNE LLHERYNYLQ GLVKMIDVRL GGNDFTIADK
IVVDYMKHKQ TGFYTVAELE GNYSVTLNKP NLSNVSEEQE KKLSEIIEEI NEQLDLDIDD
QIAVSGAVSI RELMKKNPKL KQSALVNSRE DFEFSFEDEI DKALTDGYTQ NQDFFGVLLS
NEDFKKRIAK IFIDDVYQSF KKSSD
//
