ID F0VJK2_NEOCL Unreviewed; 2300 AA.
AC F0VJK2;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=Serine/threonine-protein phosphatase {ECO:0000256|RuleBase:RU004273};
DE EC=3.1.3.16 {ECO:0000256|RuleBase:RU004273};
GN ORFNames=BN1204_036950 {ECO:0000313|EMBL:CEL67911.1}, NCLIV_036950
GN {ECO:0000313|EMBL:CBZ53913.1};
OS Neospora caninum (strain Liverpool).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Sarcocystidae; Neospora.
OX NCBI_TaxID=572307 {ECO:0000313|EMBL:CBZ53913.1, ECO:0000313|Proteomes:UP000007494};
RN [1] {ECO:0000313|EMBL:CBZ53913.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Liverpool {ECO:0000313|EMBL:CBZ53913.1};
RA Aslett M.;
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CBZ53913.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Liverpool {ECO:0000313|EMBL:CBZ53913.1};
RA Reid A.J., Sohal A., Harris D., Quail M., Sanders M., Berriman M.,
RA Wastling J.M., Pain A.;
RT "Comparative genomics and transcriptomics of Neospora caninum and
RT Toxoplasma gondii.";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000007494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Liverpool {ECO:0000313|Proteomes:UP000007494};
RX PubMed=22457617; DOI=10.1371/journal.ppat.1002567;
RA Reid A.J., Vermont S.J., Cotton J.A., Harris D., Hill-Cawthorne G.A.,
RA Konen-Waisman S., Latham S.M., Mourier T., Norton R., Quail M.A.,
RA Sanders M., Shanmugam D., Sohal A., Wasmuth J.D., Brunk B., Grigg M.E.,
RA Howard J.C., Parkinson J., Roos D.S., Trees A.J., Berriman M., Pain A.,
RA Wastling J.M.;
RT "Comparative genomics of the apicomplexan parasites Toxoplasma gondii and
RT Neospora caninum: Coccidia differing in host range and transmission
RT strategy.";
RL PLoS Pathog. 8:e1002567-e1002567(2012).
RN [4] {ECO:0000313|EMBL:CEL67911.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Liverpool {ECO:0000313|EMBL:CEL67911.1};
RX PubMed=25875305; DOI=10.1371/journal.pone.0124473;
RA Ramaprasad A., Mourier T., Naeem R., Malas T.B., Moussa E., Panigrahi A.,
RA Vermont S.J., Otto T.D., Wastling J., Pain A.;
RT "Comprehensive Evaluation of Toxoplasma gondii VEG and Neospora caninum LIV
RT Genomes with Tachyzoite Stage Transcriptome and Proteome Defines Novel
RT Transcript Features.";
RL PLoS ONE 10:e0124473-e0124473(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC Evidence={ECO:0000256|RuleBase:RU004273};
CC -!- SIMILARITY: Belongs to the PPP phosphatase family.
CC {ECO:0000256|ARBA:ARBA00008294, ECO:0000256|RuleBase:RU004273}.
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DR EMBL; FR823390; CBZ53913.1; -; Genomic_DNA.
DR EMBL; LN714483; CEL67911.1; -; Genomic_DNA.
DR RefSeq; XP_003883945.1; XM_003883896.1.
DR GeneID; 13443560; -.
DR VEuPathDB; ToxoDB:NCLIV_036950; -.
DR eggNOG; KOG0374; Eukaryota.
DR InParanoid; F0VJK2; -.
DR OrthoDB; 206845at2759; -.
DR Proteomes; UP000007494; Chromosome VIII.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.60.21.10; -; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR006186; Ser/Thr-sp_prot-phosphatase.
DR PANTHER; PTHR11668; SERINE/THREONINE PROTEIN PHOSPHATASE; 1.
DR PANTHER; PTHR11668:SF508; SERINE_THREONINE-PROTEIN PHOSPHATASE; 1.
DR Pfam; PF00149; Metallophos; 1.
DR PRINTS; PR00114; STPHPHTASE.
DR SMART; SM00156; PP2Ac; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF56300; Metallo-dependent phosphatases; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS00125; SER_THR_PHOSPHATASE; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Hydrolase {ECO:0000256|RuleBase:RU004273};
KW Reference proteome {ECO:0000313|Proteomes:UP000007494}.
FT DOMAIN 1118..1153
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..226
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 296..366
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 436..465
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 471..490
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 521..593
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 628..662
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 703..722
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 855..1007
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1223..1249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1294..1314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1750..1794
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1839..1908
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1972..1993
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2038..2095
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2107..2271
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2281..2300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..101
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..140
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..313
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 855..906
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 932..975
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1773..1788
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1890..1907
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2184..2211
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2300 AA; 240739 MW; F51A0E1E44CB0B89 CRC64;
MDSAATERGA RLAPKAPISS PSAAVSPSNV ELFPVLASSL SEAKLPDKRS LLFLHASPME
TSLPSPPGCF SRRRSLEAAH AEQDPPRNVA CVEDREGLSH PASFDDPQGA FRAAAPSRDQ
AELLQIHQRL REEALQRQRE GHSSEAGAPP ADVSQESAKT YHYVPNALAH GPSHAAENRS
PHGLPQSVAP AHAARPLFTG NSPTCFSSGP RAEGRTDGAW TETGGTRTWV VSEPRPAASS
GTYASSVQAA PPALAPSLYV QPMASVDPQK HTRLVSPAFA SALAPTLEQR YAVGAPASCA
STTSGSSAYP SSLRREAPMS RSRVMVRDSG TGAYLSPARS PDGPFSRADG GRGAATKERR
RGRKKRLWGG RWGEKLQAFY ESGESECDSA GGEDTQEAET HVGEATVSGF AGEAPTWTSP
SVVSTLFSVF SNPERAASRD SRVSVHPMSP IQGAPAGPRP DAHLLSAFPC ETPRQAHSGS
PAHPRVLSDG QVYIPGTGDF RVSALAASAS GSSQAVSVAS PTFAARGHSP SAASRSRGGY
LPSGTSPRVS GVERGRSPGV PDPGARFGAA SPVQPRRRRR QRKAKGGDGA KRSFSETAGA
VAANAGAAVV GAIGGFVGGV VDHIIESHSE ASRSRSPRDS SLFSSASEHG ARSGDEADGF
AHPHAALEPV PRFDLQTGNR LQNPGALTAA AALSGGLPAP WQGAPGFGGS SNPGSVLPGL
SRQDSLASAH GAHAPGLLPR AAVSPCLQTG VSASVCPSVG ASVSGASAPG QPALPLRAPL
PFPRSAAPPS LTVEEKGRAL GNITASVVLE NLSLTARSLL HVASVAPGLA ASVALAAADV
VKAVKRGMEG VEGDDAWELE HKLSPRPRER SEERGQEETP KKLGTDRGVR EDTGAGTDRD
ASQGRAAKNE SVEAGTCGDS SEKPTPKGRK QAAQSRRASP SAGARQVEQG RTSAAPTPSA
RQASRESSPR QAAQQSDARL GKLRGDSPTN SPKDAAGGAA SQLPPTETEA AELAAAFREW
QSFGFLWDRV VLEECCVEEA QRLKQLFDRF AEPSSPPRLS REGFAKLLAA YPCLPPEHHD
FFFRTLARRS EDSILLRDFR AGFYVAQPQL PEEVERASGK LRLQFVFKAY DLDGDGWLQE
EELRGLLSHL HSAGLHAEDL KIKQDRDALD KLVKGEAQVL LQRFPRFGYS AFLQCVRSGV
FNATHRLLRC RASLPGLVKA EERTRRRSCA ARPSPQVPAK STVAESTPSL EAQSPATAVV
SAAAAAVSAA AAASAVATAA FAHDCPERRT QRLLPASRSP SPEAVHGQGW PSTAAGVAAP
RAPAVCLAGG AQLADARWVG AYPGEASSEA GPRPLTTPEK PVAKKAAYPS VPAQQTRVAQ
KIVHHVKTCL PFVSRADLDA RLPALLSVNA SSPAHAGDRE GAPSPSFLQM PLAVEEVLAL
CDAVERLVAA EETVIELDVP LKIYGDLHGH LADLLEFFGS FGWPGDEDKL TEQIEDFLFL
GDYVDRGPCS LEVLLLLFSL KVLSPNRVFL LRGNHEDRQM NRVYGFLEEL ERKLGGDAST
RVWEKANEVF DLLPLAALVP VAGVFCLHGC LGDSIETVDD LRVIQRPLDI SVLQLDGVDA
GARGASAADH KVLDCLWSDP ERPPERAEDA AGPASPRGGH VVRSSAAFIE AFLERNRLAL
LVRGHECVAP GYCYDLGGRC LTLFSASNYC GTANNDGAAL HIYREEEPLP NPDRPRQHIT
RHILLVERQD RSRAGVSGLP PQTRFSPQGF PTPLGSPSGP PPTRPPPLHG GEVGGAMFSE
LTRLASEAAR SPLGSSSFPS SAEAAALLSS ALRSSTTVPL TSSSAFGEPP LASPSQHEKI
DADGPFGPST LGAWLASPGP HIEIPSEFLS SHSPPHSPLS SSPLTASGPR AAARASASVS
AALQGSFGLP AASLHGDGWV SPLSPAYLAD GLRESGATWM QAVSPGRDTA ATAGLEGADA
SPPSPRPAHL SEEMQNAHPP LPVASPLVPI PTVSEVFSEG EEGGDRGLPT PRLGARAETE
IGGSRGPAFP GADSPSSAAR RVFSGASEGG QAPAGRTRPN LQVLEPPHRE ECSPLSPHWR
AVHGILAPTS NPADSRLPEE GHGEVTDGSA SGPGSPRRTR RLTSRFALAD VDHPGAALPP
DVVPQSRPPA FSPPSSSLGD DGARCLRSRS EEARERDQLS LSDRDRSPRG ADPSGSRVSL
GECRDDTERA TASSAAFSRG GGGRRAAGWR GPEPRGRRSL PAGGSRWGGE EDDPVAAAFL
AAAQAGPSRK RAGRRKSFAG
//
