ID F0Y2J4_AURAN Unreviewed; 1114 AA.
AC F0Y2J4;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 03-MAY-2023, entry version 50.
DE RecName: Full=subtilisin {ECO:0000256|ARBA:ARBA00023619};
DE EC=3.4.21.62 {ECO:0000256|ARBA:ARBA00023619};
GN ORFNames=AURANDRAFT_71024 {ECO:0000313|EMBL:EGB11061.1};
OS Aureococcus anophagefferens (Harmful bloom alga).
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Pelagophyceae; Pelagomonadales;
OC Aureococcus.
OX NCBI_TaxID=44056 {ECO:0000313|Proteomes:UP000002729};
RN [1] {ECO:0000313|EMBL:EGB11061.1, ECO:0000313|Proteomes:UP000002729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP 1984 {ECO:0000313|Proteomes:UP000002729};
RX PubMed=21368207; DOI=10.1073/pnas.1016106108;
RA Gobler C.J., Berry D.L., Dyhrman S.T., Wilhelm S.W., Salamov A.,
RA Lobanov A.V., Zhang Y., Collier J.L., Wurch L.L., Kustka A.B., Dill B.D.,
RA Shah M., VerBerkmoes N.C., Kuo A., Terry A., Pangilinan J., Lindquist E.A.,
RA Lucas S., Paulsen I.T., Hattenrath-Lehmann T.K., Talmage S.C., Walker E.A.,
RA Koch F., Burson A.M., Marcoval M.A., Tang Y.Z., Lecleir G.R., Coyne K.J.,
RA Berg G.M., Bertrand E.M., Saito M.A., Gladyshev V.N., Grigoriev I.V.;
RT "Niche of harmful alga Aureococcus anophagefferens revealed through
RT ecogenomics.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:4352-4357(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of proteins with broad specificity for peptide
CC bonds, and a preference for a large uncharged residue in P1.
CC Hydrolyzes peptide amides.; EC=3.4.21.62;
CC Evidence={ECO:0000256|ARBA:ARBA00023529};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU01032};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PROSITE-
CC ProRule:PRU01032};
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DR EMBL; GL833123; EGB11061.1; -; Genomic_DNA.
DR RefSeq; XP_009034615.1; XM_009036367.1.
DR AlphaFoldDB; F0Y2J4; -.
DR EnsemblProtists; EGB11061; EGB11061; AURANDRAFT_71024.
DR GeneID; 20228038; -.
DR KEGG; aaf:AURANDRAFT_71024; -.
DR eggNOG; KOG1012; Eukaryota.
DR InParanoid; F0Y2J4; -.
DR OrthoDB; 1405251at2759; -.
DR Proteomes; UP000002729; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00030; C2; 1.
DR CDD; cd04056; Peptidases_S53; 1.
DR CDD; cd11377; Pro-peptidase_S53; 1.
DR CDD; cd06974; TerD_like; 2.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR Gene3D; 2.60.60.30; sav2460 like domains; 2.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015366; S53_propep.
DR InterPro; IPR030400; Sedolisin_dom.
DR InterPro; IPR003325; TerD.
DR PANTHER; PTHR14218; PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2; 1.
DR PANTHER; PTHR14218:SF15; TRIPEPTIDYL-PEPTIDASE 1; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR Pfam; PF09286; Pro-kuma_activ; 1.
DR Pfam; PF02342; TerD; 2.
DR SMART; SM00239; C2; 1.
DR SMART; SM00944; Pro-kuma_activ; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF54897; Protease propeptides/inhibitors; 1.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS51695; SEDOLISIN; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|PROSITE-
KW ProRule:PRU01032}; Hydrolase {ECO:0000256|PROSITE-ProRule:PRU01032};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|PROSITE-
KW ProRule:PRU01032}; Protease {ECO:0000256|PROSITE-ProRule:PRU01032};
KW Reference proteome {ECO:0000313|Proteomes:UP000002729};
KW Serine protease {ECO:0000256|PROSITE-ProRule:PRU01032}.
FT DOMAIN 19..147
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 740..1114
FT /note="Peptidase S53"
FT /evidence="ECO:0000259|PROSITE:PS51695"
FT ACT_SITE 816
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01032"
FT ACT_SITE 820
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01032"
FT ACT_SITE 1031
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01032"
FT BINDING 1073
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01032"
FT BINDING 1074
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01032"
FT BINDING 1092
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01032"
FT BINDING 1094
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01032"
SQ SEQUENCE 1114 AA; 117794 MW; 9D4350A9DDBF8BB5 CRC64;
MASLVDNMDG LAVADAVVVA GGVSGTVAGT SAEHMMNPGT LEIEVIQGRD LVIKDRGTFR
SNKSDPFCVV AVDGAKVGKT KTVDRNLSPV WNFSTAAKVK RGAQKRLVVN CFDKDKLSSS
DPMGTVVIEV LEALRGADVA TRVRRWYDVE NCEGCDNARG QIEIAFAWKP KTVIALEKGT
PFRVEHPEQA LFVGLGWTGA CGAKVDLDAS CVFFDDQGAV VDALYFGNTQ CFDGAALHSG
DALTGDEAAA EEDADERIEL RLAKLPRAVA SMLFVVTAYA EKSSFVDMKS AFVGLFDPVE
GEMCRYAFDC RGDHTGLVMC RVARSGPAWV LNAIGDVAAG PRDYGTWVPE LKAYLSDLVS
HVRVGDPNDR VAIMHKGSVV DLSYYQAGPL AEVRMGVAWD ITGGRSIDLD ASCLLLKAGC
SEATTEIVSY QKLSSSDGRV RHSGDDTTGD GGGDDEVITV ELDKLAPDVR YVAFVVNSYS
GQPFSQVDNV SCHLFLPPSR ARPKPQDLAI FNLSSKTYHT TALVMTILER STRPDGSPTW
LMRAVGEGTE AKVAKQCLDE IQLVRGERVA ASALIELEFW LKHDAADLAA FHDDLVERST
PGSAKYSDWL SKEEVRAMLA PSREALDAVL DYVVHDLGAA DVHVDDFKSV VSVAVPAGAV
ERALDTKLYA HAHVDYAHVE VIRVGEAYSL PAAVAAHVSL VSELVRFPRL RRSDLVAAAV
DAAPNATGAW AKCGAKNSAY TNPYVLAERY GFEFPLTDAA DGNSMAVAEF QGQYWDPKDL
GAFSTACGLP SAISVSKTVG GNVPLLCEGL GQGCVESLLD IEYAGSIAGA IPLEVYYSGT
YSLLAWANKL GDATPAPLVN SVSYGNDEAQ QTGSALCSAY MESVNAAFMK VSNAGGKRVV
SNTGVSILFA AGDQGVWGRE GPGLKYHPDF PAASPYVTAV GGTDFATKSV IGDETTWNDG
GSGFSNEFAQ PAWQADDVAA YLKSATGLPK ARMYNATGRA YPDVAALAGL VNPYLVALSG
GKSFAGVGGT SAASPTVAAM IAQVNNNRLK AGKKSMGWLN PFLYKTGEAA FHDVTTGKTS
GGFTGGFPAA PGWDAATGFG TVDFKKLNAA ALAA
//
