ID F1M8G6_RAT Unreviewed; 3132 AA.
AC F1M8G6;
DT 03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 03-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 89.
DE RecName: Full=DNA polymerase zeta catalytic subunit {ECO:0000256|ARBA:ARBA00021589};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=Rev3l {ECO:0000313|Ensembl:ENSRNOP00000054053.2,
GN ECO:0000313|RGD:1307087};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116 {ECO:0000313|Ensembl:ENSRNOP00000054053.2, ECO:0000313|Proteomes:UP000002494};
RN [1] {ECO:0000313|Ensembl:ENSRNOP00000054053.2, ECO:0000313|Proteomes:UP000002494}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000054053.2,
RC ECO:0000313|Proteomes:UP000002494};
RX PubMed=15057822; DOI=10.1038/nature02426;
RG Rat Genome Sequencing Project Consortium;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2] {ECO:0000313|Ensembl:ENSRNOP00000054053.2}
RP IDENTIFICATION.
RC STRAIN=Brown Norway {ECO:0000313|Ensembl:ENSRNOP00000054053.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755}.
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DR RefSeq; NP_001077435.1; NM_001083966.1.
DR SMR; F1M8G6; -.
DR STRING; 10116.ENSRNOP00000054053; -.
DR PaxDb; 10116-ENSRNOP00000054053; -.
DR Ensembl; ENSRNOT00000057227.4; ENSRNOP00000054053.2; ENSRNOG00000000593.8.
DR GeneID; 309812; -.
DR KEGG; rno:309812; -.
DR AGR; RGD:1307087; -.
DR CTD; 5980; -.
DR RGD; 1307087; Rev3l.
DR eggNOG; KOG0968; Eukaryota.
DR GeneTree; ENSGT00940000156226; -.
DR HOGENOM; CLU_000203_1_0_1; -.
DR InParanoid; F1M8G6; -.
DR OMA; DPTSWIR; -.
DR OrthoDB; 211439at2759; -.
DR TreeFam; TF101072; -.
DR Reactome; R-RNO-110312; Translesion synthesis by REV1.
DR Reactome; R-RNO-5655862; Translesion synthesis by POLK.
DR Reactome; R-RNO-5656121; Translesion synthesis by POLI.
DR Proteomes; UP000002494; Chromosome 20.
DR Bgee; ENSRNOG00000000593; Expressed in quadriceps femoris and 20 other cell types or tissues.
DR ExpressionAtlas; F1M8G6; baseline and differential.
DR Genevisible; F1M8G6; RN.
DR GO; GO:0005730; C:nucleolus; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0016035; C:zeta DNA polymerase complex; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; ISO:RGD.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0042276; P:error-prone translesion synthesis; ISO:RGD.
DR CDD; cd05778; DNA_polB_zeta_exo; 1.
DR CDD; cd05534; POLBc_zeta; 1.
DR CDD; cd22287; REV3L_RBD; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 3.30.342.10; DNA Polymerase, chain B, domain 1; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR032757; DUF4683.
DR InterPro; IPR030559; PolZ_Rev3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR025687; Znf-C4pol.
DR PANTHER; PTHR45812; DNA POLYMERASE ZETA CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR45812:SF1; DNA POLYMERASE ZETA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 2.
DR Pfam; PF15735; DUF4683; 1.
DR Pfam; PF14260; zf-C4pol; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000002494};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 95..187
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 746..1132
FT /note="DUF4683"
FT /evidence="ECO:0000259|Pfam:PF15735"
FT DOMAIN 2275..2486
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 2552..3002
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 3044..3111
FT /note="C4-type zinc-finger of DNA polymerase delta"
FT /evidence="ECO:0000259|Pfam:PF14260"
FT REGION 425..458
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 483..509
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 524..549
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 840..874
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1034..1076
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1153..1285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1427..1452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1536..1614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1840..1867
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1957..1978
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2089..2136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..452
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..499
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1040..1059
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1182..1203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1235..1268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1536..1554
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1569..1583
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2097..2118
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3132 AA; 351898 MW; 8BACCEAEEF6A1CF1 CRC64;
MFSVRIVTAD YYMASPLPGL DICQSPLTQH PVKKVPVVRV FGATPAGQKT CLHLHGVFPY
LYVPYDGYGQ QPERYLSQMA LSIDRALNVA LGNPSSTAQH VFKVSLVSGM PFYGYHEKER
HFMKIYLYNP AMVKRICELL QSGAIMNKFY QPHEAHIPYL LQLFIDYNLY GMNLINLAAV
KFRKARRKGD ASHATGLFKP QLSGNSPAGT LFRWEEDEIP SSLLLEGVEP LSTCELEVDA
VAADILNRLD IEAQIGGNPG LQAIWEDEKQ RRRNRNENSQ ISQPESQDCR LVPATESEKI
FQKRLQEILK QNDFSVTLSG SVDYSSGSQE FSAELTLHSE ILSPEMLPCS PANMIEVHRD
NDWSKGNTKH KVEEALINEE AILNLIENSQ TFQPLTQRLS ETPVFMDSSP DESLVHLLAG
LESDGYQGEK NRMPLPRHTF GESQNPQNSD DEENAPQMEK EEMELSVLMS QRWDSDTEEQ
CAKKRSLCRN AHRSSTEEDD SSSEEEMEWT DNSLLFANLS IPQLDGTADE NSDNPLNNEN
SRAHSSVIAT SKLSVRPSVF HKDAATLEPP SSAKITFQCK HTSALSSHGL NKDDLIEGLS
QPNNTEKGRD SSVTFTKEST YSMKYSGSLS STVHSDNSHK EICKKDKSLP VPSCESSVFD
YEEDIPSVTR QAPSRKYSNM RKIEKDAPFI HANRHTSEST LGKNSFNFAD LNHSKNKLSS
EGNERGNSTA LNSVFPSSLA ESCDLLSSSG ENRSVAHSLD SITDESGRSK LKIRYEEFQE
HKMERPSLSQ QAAHYMFFPS VVLSNCLTRP QKLSPVTYKL QPGSKTSRLK LNKKKLISLQ
ETSTKSSETG AAKDSCTHNH PYSGTPEKES GLSSDLIKAT HGTFENKTPT EHFIDCHFGD
GSLETEQSFG LYGNKYTLRA KRKVNYETED SESSFVTHNS KISLPHPMEI GENLDGTLKS
RKRRKMSKKL PPVIIKYIII NRFRGRKNML VKLGKIDSKE KQVILTEEKM ELYKKLAPLK
DFWPKVPDSP ATKYPIYPLT PKKSHRRKSK HKSAKRKPGK QHRASSENIK RTLSFRKKRT
HAVLSPPSPS YIAETEDCDL NYSDVMSKLG FLSERSTSPV NSSPPRCWSP TDPRAEEIMA
AAEKESVLFK GPNVCNTKTV SPRVGKASRA RAQVKKSKAR LAKSSVVTNK RNKRNQTNQL
VDNGKKKPRA KQKQRANEKG ISRKPVIPTD ETTKPPSEAE LTPSQRSVPE LTSSSGIHTL
SKQKEMTQTG PAMDHPLPPC QPTGINAQQR LSNCFSSFLE SRKSVQFPTF PSSRGDSRSS
VYSSIGPGVS KVNTQRSHSQ SAMFTRKETT LIQKSIFDLS NHLSQVAQST QLCSGIISPK
TEESSNTQKN CGSSIGKLNE YRSSLESKPE KVCAPNFLHC RDSQQQTVNV SEQSKPSETC
SPGNAASEES QTPNCFVTSL RSPIKQITWE QRQRGCILEM SSFKPEKVKQ RSLSEAISQT
KALSQCKNQN VSTPSVFGEG QSGLAVLKEL LQKRQQKAQT TNVQDSASSH QPDKDISVSA
EHKKTNKRTR AVASPRKPRA PRRTKPKEQT PRRLKVDPLN LQTSGHLDNS LSDDSPILFS
DPGFESCYSL EDSLSPEHNY NFDINTIGQT GFCSLYSGSQ FVPADQNLPQ KFLSDAVQDL
FPGQAIEKNE LLSHDRQSCT EEKHHVSDSG PWVRASTLNP ELFEKVGIDN NENHRHSQWK
TSFHPLTSHS NSIMESFCVQ QAESCLPEKS RLNRSSVSKE VFLSLPQASS SDWIQGHNGK
EIEQSLDSAN TSFTTVLSSP DGELVDAASE DLELYVSRNN DVLTPTPDSS PRSTSSPLQS
KNGSFTPRTA HILKPLMSPP SREEIVATLL DHDLSEAIYQ EPFCSNPSDV PEKPREIGGR
LLMVETRLPN DLIEFEGDFS LEGLRLWKTA FSAMTQNPRP GSPLRNGPAV VNKESSNSHK
TVEDKKIVIM PCRYAPSRQL VQAWLQAKEE YVRSKRLPRT ESTPVIKSAD NVSPSLNPGD
TRAVAPQMDT CPHTLSSTAH AKEEVCQSQI ALQTSRTGCG QTVSASQTLL PAASAAVPEE
EEEEEEEEEE EEEDENGYVS YSSPDSPGIP PWQQAASPDF RALNGEHRCS SPVEELCSRA
VENFLKPIKD GIQKASCCES QVISPIHART RTEKCEPLCL HSTPVMPRKF LEKLPEATGL
SPLSVEPKTQ KLYNKKGSDT DHLRRVLLTT QVENQFAAVN TPKKETSQID GPSLNNTYGF
KVSIQNLQEA KALHEIQNLT LISVELHART RRDLQPDPEF DPICALFYCI SSDTPLPDTE
KTELTGVIVI DKDKTVTHQD IRCQTPLLIR SGVTGLEVTY AADEKALFQE VTNIMKRYDP
DILLGYEIQM HSWGYLLQRA AALSVDLCQM ISRVPDDKIE NRFAAERDDY GSDTMSEINI
VGRITLNLWR IMRNEVALTN YTFENVSFHV LHQRFPLFTF RVLSDWFDNK TDLYRWKMVD
HYVSRVRGNL QMLEQLDLIG KTSEMARLFG IQFLHVLTRG SQYRVESMML RIAKPMNYIP
VTPSVQQRSQ MRAPQCVPLI MEPESRFYSN SVLVLDFQSL YPSIVIAYNY CFSTCLGHIE
NLGKYDEFKF GCTSLRVPPD LLYQIRHDIT VSPNGVAFVK PSVRKGVLPR MLEEILKTRF
MVKQSMKAYR QDRALSRMLN ARQLGLKLIA NVTFGYTSAN FSGRMPCIEV GDSIVHKARE
TLERAIKLVN DTKKWGARVV YGDTDSMFVL LKGATKEQSF KIGQEIAEAV TATNPKPVKL
KFEKVYLPCV LQTKKRYVGY MYETLDQKDP VFDAKGIETV RRDSCPAVSK ILERSLKLLF
ETRDISLIKQ YVQRQCMKLV EGKASIQDFI FAKEYRGSFS YRPGACVPAL ELTRKMLAYD
RRSEPRVGER VPYVIVYGTP GLPLIQLIRR PAEVLQDPTL RLNATYYITK QILPPLARIF
SLIGIDVFSW YQELPRIQKA TSSSRSDLEG RKGTISQYFT TLHCPVCDGL TQHGICSQCR
SQPQHVAIIL NQEIRELERQ QEQLVKICRN CTGSFDRHIP CVSLNCPVLF KLSRVNRELS
KAPYLRQLLD QF
//
