ID   F1S0D7_PIG              Unreviewed;      1898 AA.
AC   F1S0D7;
DT   03-MAY-2011, integrated into UniProtKB/TrEMBL.
DT   11-DEC-2019, sequence version 4.
DT   27-MAR-2024, entry version 75.
DE   RecName: Full=Lactase-phlorizin hydrolase {ECO:0008006|Google:ProtNLM};
GN   Name=LCT {ECO:0000313|Ensembl:ENSSSCP00000016633.4};
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823 {ECO:0000313|Ensembl:ENSSSCP00000016633.4, ECO:0000313|Proteomes:UP000008227};
RN   [1] {ECO:0000313|Ensembl:ENSSSCP00000016633.4, ECO:0000313|Proteomes:UP000008227}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Duroc {ECO:0000313|Ensembl:ENSSSCP00000016633.4,
RC   ECO:0000313|Proteomes:UP000008227};
RG   Porcine genome sequencing project;
RL   Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSSSCP00000016633.4}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
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DR   SMR; F1S0D7; -.
DR   STRING; 9823.ENSSSCP00000016633; -.
DR   PeptideAtlas; F1S0D7; -.
DR   Ensembl; ENSSSCT00000017091.5; ENSSSCP00000016633.4; ENSSSCG00000015691.5.
DR   GeneTree; ENSGT00940000155324; -.
DR   HOGENOM; CLU_001859_5_1_1; -.
DR   InParanoid; F1S0D7; -.
DR   TreeFam; TF314803; -.
DR   Proteomes; UP000008227; Chromosome 15.
DR   Bgee; ENSSSCG00000015691; Expressed in oocyte and 5 other cell types or tissues.
DR   ExpressionAtlas; F1S0D7; baseline.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000016; F:lactase activity; IBA:GO_Central.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 4.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR018120; Glyco_hydro_1_AS.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR   PANTHER; PTHR10353:SF38; LACTASE_PHLORIZIN HYDROLASE; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 3.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 4.
DR   PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 2.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 3.
PE   1: Evidence at protein level;
KW   Glycosidase {ECO:0000256|RuleBase:RU004468};
KW   Hydrolase {ECO:0000256|RuleBase:RU004468};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:F1S0D7};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008227};
KW   Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           20..1898
FT                   /note="Lactase-phlorizin hydrolase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5023907246"
FT   TRANSMEM        1854..1874
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   REGION          1194..1215
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        1247
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
FT   ACT_SITE        1720
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
SQ   SEQUENCE   1898 AA;  214699 MW;  06195019235E318C CRC64;
     MELAWHLVSI VLLSFSCWGL DWESDRNFIL AAGPLTTDLL YDLRGPLGHQ DSHLEQETKV
     FMCVASHCLM ITHYKVFLPW AQLLPEGSSK NPDKGMVQCY RQLLETLPAS TVQRSEAFAD
     LFADYASFVF HSFGDLVKIW FTFSDLEEVI TELPHQESRA SHLQILAEAH RKAYEIYHEK
     YSSQGGKLSV VLRAEAVSQL LPEPSTSVLA KDAVDFLSLD LSSECQESET SLPQKLRKLQ
     TIEPNVKLFI FSLKLQDCLS TRKNPADVLF SLLKALNEDQ VFTIGFDIHT FLSCSSSSKK
     SRSCSLTDSL TLQAALQLDP ETAVDSSPPR SAYQAVWETF ANQSRAERDA LLQGVFPEGF
     LWGVSTGAFN VEGGWAEDGR GASVWDRLGH QDTAKGQATP EVASDSYHKV DTDVALLRGL
     RAQVYKFSIS WSRIFPSGQG HSPNLQGVAY YNKLIDSLLD SHIEPMATLF HWDLPQALQD
     RGGWQNESVV DAFLDYAAFC FSTFGDRVKM WVTFHEPWVM SYAGYGTGQH APGISDPGVA
     SFKVAHLVLK AHARAWHHYN SHHRPRQQGR VGIVLNSDWA EPLSPERPED LRASERFLHF
     MLGWFAHPIF VDGDYPATLR AQVQRVNKGC PSPVAQLPEF TEVEKQLLKG SADFLGLSHY
     TSRLISKAHQ DTCIPSYDTI GGFSQHVDPT WPQTASPWIR VVPWGIRRLL RFVSLEYTRG
     KVPIYLAGNG MPIGEGEDLL HDSSRVTYFN QYINEVLKAV KEDSVDVRSY IARSLLDGFE
     GPSGYSQRFG LYHVNFNDSS RSRTPRKSAY FLTSMIEKNS LLSKAVKEQP PSSRADIPQK
     IRAFAFPSEV PSKAKVVWEK FSNQPKFERD LFYHGTFRDD FLWGVSSSAY QIEGAWDADG
     KGPSIWDNFT HTPGSNVKDN ATGDVACDSY NHLNADLNML RALKVKAYRF SISWSRVFPT
     GRNSSINTRG VDYYNRLIDG LVASNISPMV TLFHWDLPQA LQDIGGWENP ALTELFNSYA
     DFCFQTFGDR VKFWMTFNEP TYQAWLGYGS GDFPPNVKDP GSGPYRIGHA ILKAHATVYH
     TYDEKYRQEQ KGVISLSLST HWAEPQSPGV PRDVEAADRM LQFSLGWFAH PIFRNGDYPD
     AMKWKVGNRS ELQHLATSRL PSFTEQEKAY IRATADVFCL NTYSSRIVRH ATPRLNPPSY
     EDDQELTEEE DPSWPSTAVN RAASWGMRRL LNWIKEEYGD IPIYITENGV GLTDPGVEDT
     DRIFYHKTYI NEALKAYRLD GVDLRGYAAW SLMDNFEWLN GYTVKFGLYH VDFNNVNRPR
     TARASARYYT EVITNNGMPL PAEDEFLYGQ FPEGFIWSAA TAAYQIEGAW RADGKGLSIW
     DTFSHTPLRI GNDDTGDVAC DSYHKIAEDV VALQNLGVSH YRFSISWTRI LPDGTTKYIN
     EAGLNYYVRL IDALLAANIQ PQVTIYHWDL PQALQDVGGW ENETIVQRFK EYADVLFQRL
     GDKVKFWITL NEPFVVAQQG YGYGISAPGI SFRPGTAPYI VGHNLIKAHA EAWHLYNDVY
     RASQGGVISI TINSNWAEPR DPSNQEDVEA ARRYVQFMGG WFSNPIFKNG DYPEVMKTRI
     RDRSLAGRAG SNLFTESEKR RINGTYDFFG FNHYTTVLAY SFNYATWLSS FDADRGVASI
     TDRSWPDSGS FWLKMTPFGF RRILNWLKEE YNNPPIYVTE NGVSQREESD LNDTARIYYL
     RSYLNEALKA VQDKVDLRGY TVWTLMDNFE WATGFAERFG LHFVNYTDTS LPRIPKASAK
     FYASIVRCNG FPDPAAGPHP CLQQEGAEPT ISPMSQEDVQ FLGLTLGTTE AQTALYVLFA
     LLLLGVCGVA FLSYKYCKRS KQKETQPGEQ ELNRISSF
//