ID F2KAC9_PSEBN Unreviewed; 317 AA.
AC F2KAC9;
DT 31-MAY-2011, integrated into UniProtKB/TrEMBL.
DT 31-MAY-2011, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=Glycine--tRNA ligase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00254};
DE AltName: Full=Glycyl-tRNA synthetase alpha subunit {ECO:0000256|HAMAP-Rule:MF_00254};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00254};
GN Name=glyQ {ECO:0000256|HAMAP-Rule:MF_00254};
GN ORFNames=PSEBR_a12 {ECO:0000313|EMBL:AEA66128.1};
OS Pseudomonas brassicacearum (strain NFM421).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=994484 {ECO:0000313|EMBL:AEA66128.1, ECO:0000313|Proteomes:UP000006692};
RN [1] {ECO:0000313|EMBL:AEA66128.1, ECO:0000313|Proteomes:UP000006692}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NFM421 {ECO:0000313|EMBL:AEA66128.1,
RC ECO:0000313|Proteomes:UP000006692};
RX PubMed=21515771; DOI=10.1128/JB.00411-11;
RA Ortet P., Barakat M., Lalaouna D., Fochesato S., Barbe V., Vacherie B.,
RA Santaella C., Heulin T., Achouak W.;
RT "Complete genome sequence of a beneficial plant root-associated bacterium,
RT Pseudomonas brassicacearum.";
RL J. Bacteriol. 193:3146-3146(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NFM421;
RA Ortet P., Barakat M., Lalaouna D., Fochesato S., Barbe V., Santaella C.,
RA Heulin T., Achouak W.;
RT "Complete Genome Sequence of a beneficial plant roots-associated bacterium
RT Pseudomonas brassicacearum.";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00254};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|ARBA:ARBA00011209, ECO:0000256|HAMAP-Rule:MF_00254}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00254}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00254}.
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DR EMBL; CP002585; AEA66128.1; -; Genomic_DNA.
DR RefSeq; WP_003177094.1; NC_015379.1.
DR AlphaFoldDB; F2KAC9; -.
DR SMR; F2KAC9; -.
DR STRING; 994484.PSEBR_a12; -.
DR GeneID; 57472987; -.
DR KEGG; pba:PSEBR_a12; -.
DR HOGENOM; CLU_057066_1_0_6; -.
DR Proteomes; UP000006692; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00733; GlyRS_alpha_core; 1.
DR HAMAP; MF_00254; Gly_tRNA_synth_alpha; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR InterPro; IPR002310; Gly-tRNA_ligase_asu.
DR NCBIfam; TIGR00388; glyQ; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF02091; tRNA-synt_2e; 1.
DR PRINTS; PR01044; TRNASYNTHGA.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00254};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00254}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00254};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00254};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00254};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00254}.
SQ SEQUENCE 317 AA; 36247 MW; 0BB3AFD6CCB2A600 CRC64;
MSQPTPAVRT FQDLILALQQ YWAEQGCVVL QPYDMEVGAG TFHTATFLRA IGPETWNAAY
VQPSRRPTDG RYGENPNRLQ HYYQFQVVLK PNPENFQELY LGSLKHVGLD PLVHDIRFVE
DNWESPTLGA WGLGWEVWLN GMEVTQFTYF QQAGGIECYP VTGEITYGLE RLAMYLQGVD
SVYDLVWADG PFGKVTYGDV FHQNEVEQST YNFEHANVEK LFELFDFYES EAKRLIELDQ
PLPLPSYEMV LKASHTFNLL DARRAISVTA RQQYILRVRT LARSVAQAYL LARAKLGFPM
ATPDLRDEVL AKLEAAQ
//
