ID F3CC70_PSESG Unreviewed; 495 AA.
AC F3CC70;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=Pgy4_27890 {ECO:0000313|EMBL:EGH16862.1};
OS Pseudomonas savastanoi pv. glycinea str. race 4.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=875330 {ECO:0000313|EMBL:EGH16862.1, ECO:0000313|Proteomes:UP000005466};
RN [1] {ECO:0000313|EMBL:EGH16862.1, ECO:0000313|Proteomes:UP000005466}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=race 4 {ECO:0000313|Proteomes:UP000005466};
RX PubMed=21799664; DOI=10.1371/journal.ppat.1002132;
RA Baltrus D.A., Nishimura M.T., Romanchuk A., Chang J.H., Mukhtar M.S.,
RA Cherkis K., Roach J., Grant S.R., Jones C.D., Dangl J.L.;
RT "Dynamic evolution of pathogenicity revealed by sequencing and comparative
RT genomics of 19 Pseudomonas syringae isolates.";
RL PLoS Pathog. 7:E1002132-e1002132(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGH16862.1}.
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DR EMBL; ADWY01001380; EGH16862.1; -; Genomic_DNA.
DR AlphaFoldDB; F3CC70; -.
DR PATRIC; fig|875330.6.peg.4668; -.
DR HOGENOM; CLU_020164_1_1_6; -.
DR BioCyc; PSYR875330:G11XH-5325-MONOMER; -.
DR Proteomes; UP000005466; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002941; DNA_methylase_N4/N6.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR002295; N4/N6-MTase_EcoPI_Mod-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF01555; N6_N4_Mtase; 1.
DR PIRSF; PIRSF015855; TypeIII_Mtase_mKpnI; 1.
DR PRINTS; PR00506; D21N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EGH16862.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 65..405
FT /note="DNA methylase N-4/N-6"
FT /evidence="ECO:0000259|Pfam:PF01555"
SQ SEQUENCE 495 AA; 55866 MW; 7DB0B470DDF134B6 CRC64;
MPTLHWIGKN KVVNHHHEVP FRVLNHQCRY RAPEGAPPNS TDNRIIHGDN LEALKSLLPE
FEGQVDCIYI DPPYNTGNEG WAYNDNVNDP KLKKWLGEVV GKEGEDLSRH DKWLCMMYPR
IKLLHKLLSN TGVIFVSLDE NEFCRMRLVL EEIFGESNFL AQIIVQSNKR GQTYKQIAKT
HEYLICFGKT KDAALGELET NGENLPEVDS FGKYSSRELR NRNPKFGRFN RPNLFYPIYA
HLSNPDENGL FPVSAIDSGE GVSILPYNSE GIESCWRWGK EKLARDNVSN NTQMLFAKQT
RDGGWRVFEK YRKGSVKAKS IWFDTKHISE QGTTALNRLG LGEQFQFPKP VALIEDCISL
ATEDDALILD SFAGSGTTAE AVLNLNAKNG GNRRFILIET LEYAETLTAE RVRRVMAGYE
TGPKLTAGLG GQFDFFTVGE PLFLDPDTLN ETLDIAVIRD YVAYAEGVPV ADRINADNPF
SPFLLGLNSD TAWIF
//
