ID F3NJK6_9ACTN Unreviewed; 5650 AA.
AC F3NJK6;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:EGG46267.1};
GN ORFNames=SGM_3320 {ECO:0000313|EMBL:EGG46267.1};
OS Streptomyces griseoaurantiacus M045.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
OX NCBI_TaxID=996637 {ECO:0000313|EMBL:EGG46267.1, ECO:0000313|Proteomes:UP000003022};
RN [1] {ECO:0000313|EMBL:EGG46267.1, ECO:0000313|Proteomes:UP000003022}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M045 {ECO:0000313|EMBL:EGG46267.1,
RC ECO:0000313|Proteomes:UP000003022};
RX PubMed=21551298; DOI=10.1128/JB.05053-11;
RA Li F., Jiang P., Zheng H., Wang S., Zhao G., Qin S., Liu Z.;
RT "Draft genome sequence of the marine bacterium Streptomyces
RT griseoaurantiacus M045, which produces novel manumycin-type antibiotics
RT with a pABA core component.";
RL J. Bacteriol. 193:3417-3418(2011).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGG46267.1}.
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DR EMBL; AEYX01000037; EGG46267.1; -; Genomic_DNA.
DR STRING; 996637.SGM_3320; -.
DR eggNOG; COG1020; Bacteria.
DR Proteomes; UP000003022; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProt.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:UniProt.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd17646; A_NRPS_AB3403-like; 1.
DR CDD; cd17652; A_NRPS_CmdD_like; 2.
DR CDD; cd17651; A_NRPS_VisG_like; 1.
DR CDD; cd19544; E-C_NRPS; 1.
DR CDD; cd19540; LCL_NRPS-like; 3.
DR Gene3D; 3.30.300.30; -; 5.
DR Gene3D; 3.40.50.980; -; 10.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 5.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 5.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR001031; Thioesterase.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 5.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 5.
DR Pfam; PF13193; AMP-binding_C; 5.
DR Pfam; PF00668; Condensation; 5.
DR Pfam; PF00550; PP-binding; 5.
DR Pfam; PF00975; Thioesterase; 1.
DR SMART; SM00823; PKS_PP; 5.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 5.
DR SUPFAM; SSF47336; ACP-like; 5.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 10.
DR PROSITE; PS00455; AMP_BINDING; 5.
DR PROSITE; PS50075; CARRIER; 5.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 4.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000003022}.
FT DOMAIN 974..1049
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2031..2106
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3134..3208
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4212..4287
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 5286..5361
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 2520..2552
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5629..5650
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2520..2547
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5630..5650
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5650 AA; 605083 MW; 32D1CA9C44DB8F43 CRC64;
MSGSRGGRRD LMAGQLGVWH AQQLDPDNPI YNMAEYIEIR GTVDARLFEK AVRTAVSEID
CFALRFEEAP DGTPRQYAVP PADWPFHTVD LSEAEDPGAR AAEWMRADLS RPVDLRGGEL
FTEALLKVED GLFFWYQRIH HIIADGLAGS RIAAHVAAVY TALRNGTPLP GEAPRSTSVL
MDADAEYRAS ADFAQDRQYW TERLAGVPRA IGLGGREPSG TPRELVRHTL HVPAGAAAEL
RSSARRLGTS VSGLALAANA AYLHRATGQE DVVLGVPVLG RRTALRDIPG MTANLIPLRL
AVRPKDTVKE LVKQVSRGVR DALKHQRYRH EDILRDLKLV GGGRLYPLLV NIVSFDYDLT
FDGAPSRAHS LGGINFNDLS ISVYDRSSDG SMSVVAEGNP ELYDPEAVRA HAAQFLDVLN
WLARAGADER VHRVPLTSDA GQRLVPEEWN DTARETPAAT LPELLRTWAA RTPDALAVVH
EGSEVTYAEL HARANRLARL LIDRGAGPET PVAVMLHRSV DLVVALLAVL KAGGAYVPVD
PDYPAERVAY VLEDARPLLV LTSTDLAPRL GGTHPPHLAV DAPETLTALR GLADTDPTDA
ERRAPLLPAH PAYVIYTSGS TGRPKGVAVP HAGVVNWLTW MQGAYQLSSE DRVLQKTPFG
FDVSVREFFW PLLHGARIVV AKPSGHRDPA YLAELIRREG VTIAHFVPSL LQVFLREPTA
AACTALRAVF CSGEALTGEV VGQFRDVFAG LPLHNLYGPT EASVEVTAWT CEGGTGGSGV
PIGAPIWNTR VHVLDAALRP VPVGVAGELY LAGAGLARGY LGRPGLTAER FVANPYGGPG
ERMYRTGDLA RWNADGQVEY LGRTDDQVKI RGFRIELGEI EAALATHPDV AQSTVTVRED
RPGDRRLVGY LVPVRGPEGV EIAAVRRHVQ AALPEYMVPS ALVVLDALPL TVNGKLDRKA
LPAPAYQAAG AGRGPGTVQE ELLRTVFADI LGLPTVGLDD NFFELGGHSL LATRLVSRIR
SVLGAEVLLR TVFEAPTPAA LADRLTSSGE QRRALVRAER TERTPLSFAQ QRLWFLGELE
GPSATYNIPF ALRLTGSLDV DALENALRDL VERHEVLRTV FATVAGEPYQ RVLTPDEAAL
TLPVRRVTDL DAALAEAADH RFDLANEIPL RARLLRTGPE EHVLVLVVHH IAGDGWSLAP
LARDLSAAYT ARKAGRAPAW ATLSVQYADY SQWQRHLLGE EGDADSVLAR QLDHWRAALA
DLPQELALPF DRPRPALSSH RGGTVDLTVD ARLHAALTEL ARAEGVTVFM VLQAALAVLL
HRLGAGEDIP LGTPIAGRTD EALDDLVGFF VNTLVLRSDL SGAPTFAQLL ARTRETDLAA
YTHQDVPFER LVEDLAPARS MARHPLFQVM LTLQNTAAAR FDLPGLHVET LPTGPLPAKF
DLDFQIAERT AEDGTAAGLA GSLTYATDLF DHASAEAVVR RLVLVLRAVV ADPTVPVTRV
DVLHPGERER LLPRAGATTP EPAPTTLVAL LEAQVARTPD ATALVHDDSG LTYAEMNARA
NRLARALVDR GARPEARVAV LMDRSADLVV ALLAVIKTGA AYVPVDPAHP ADRVGHMLRD
AGVELLVTHR ALSDTVDEGV ARLVTDATET AHVLAAQGAG NLTQEERGAA LLPDHPAYVI
YTSGSTGRPK GVVVSHAAVT HYLDWALRAY PGLNGRTVLH SSAAFDLTVT PLYGTLLAGG
TLRIADLREG LPAGPAPTFL KVTPSHLGLL AEEPTRAFER GDLVVGGEHL TGEQLTRWRS
AHPDVLVTNE YGPTEAAVGC VTFTLGPGEP DTTGGIPIGR PAQHTGVLVL DAGLRPVPPG
VAGELYLAGA QLARGYLDRP GLTAERFVAN PYGVPGERMY RTGDLARWNG DGELEYLGRT
DDQVKIRGFR IELGEVEAAL ATHPAVARSA VVVREDRPGD RRLVAYIVPA TGNPGGVDTV
ALRAHLGSVL PEYMVPSALV ALDALPLTVN GKLDRAALPA PEYRASGDGR GPATVQEELL
CSVFAEVLGL PAVGVDDNFF ELGGHSLLAT RLVSRIRSVF GAEVPVRAVF EAPTPAVLVS
RLTTSTARRR PLVRAERAER MPLSFAQQRL WFLGELEGPS ATYNIPVAVR LTGVLEVEAL
RSALADVVGR HEVLRTVFTA DAGVPRQRIL PAPESFELPV TVVERSGVER AVAGLAVRAF
DLAAEMPLRA WLLRVAPDEH VLLMVVHHIA GDGWSMGPLA RDVSVAYRAR VAGGVPGWGP
LVVQYADYTL WQRELLGEEG DPGSVLSEQL AFWRKALDGV PQELALPFDR PRPAVASHRG
GRVEMCIPAE VHARIAELAR AEGVTVFMVL QASLAVLLSR MGAGCDIPVG TPVAGRTDEA
LDELVGFFVN TLVMRTDLSG DPTFVELLER VRENGLGAFA HQDVPFERLV EDLAPARSMA
RHPLFQVMLA LQNTAPADLD LPGIHTELLP TGEPVAKFDL SFALTESFGI ETETAIGTDS
VTGTATGADA DATTGTGIET RTETPTGTEA ETARRRPAGL LGAVDYAADL FEHDTVQALA
DRFVRVLDTL TREPHRRVGA VDILDPAERH RLLVDWNDSA RDVPAATLPE LFQAQVARTP
DAPAVVFAGA EVSYAELNAR ANRLARLLIE RGVGPESPVA VMVHRSVDLV LGFLAITKAG
GAYLPVDPDY PAERVAYVLE DARPALVLTS TDLAPRLGGA HPPHLAVDAP EILAALRGLA
DTDLTDAERL APLRPAHPAY VIYTSGSTGR PKGVTVTHRG LPSVAAAQAV DLGIAPGTRM
LQFASMSFDA SVWEMVTALC NGACLVSAPS HDLLPGPALV RLVAEHRVTH AMLTPAVLAL
LDPEELSTLR TLVTGGEAVG QSVVESWAPG RRLVNAYGPT ESTVCATSAG PLSPGPTGPP
IGGPVPNSRV YVLDSALRPV PPGVGGELYV AGAGLARGYL GRPRLTAERF VANPYGGPGE
RMYRTGDLAR WNGDGELEYL GRTDDQVKIR GFRIELGEVE AALAAHPAVG RSTVLVREDR
PGDRRLVGYL VPAGGRTDEV DTAALRAHLG GTLPEYMVPS ALVVLDALPL TVNGKLDRRA
LPVPDHRATG GGRGPATVEE EILCSVFAEV LGLPAVGVDD NFFELGGHSL LAVRVVELLR
ARGVSIDVRS LFAAPTAAGL AAAGGPAEVP VPENRIPAEA TALSPDMLPL VDLNRAELDR
IVARVPGGAP NVADVYPLAP LQEGILFHHL MGESGDAADV YVLPVLLGFD SPQRLDGFVA
ALQRVVDRHD ILRTAVLWEG LREPVQVVAR RATIPVTGVD LPEGPDEEAA GRMLAACPGT
MDIGRAPLIR VYRANTPVNG QWLALVQAHH LIADHTTLDV LLREVRAFLT GRENTLPVPL
PFRTFVAQSR LRVTEEEHKR YFSALLGDVT EPTAPFGLLD VRGDGSGVTE TRRVMDTGLS
RRLREQSRRL GVSPATILHV VWARVLAATS GREDVVFGTV LFGRMAAGSG ADRVLGLFIN
SLPVRVRTHE ARVEDTVRAV QNQLADLLVH EHAPLKLARQ AGGIKADTPL FTSLFNYRHS
PSTDPGTEHA LDGVELLYDQ ERTNYPIGLS VDDTGEDFAL ALQAAAPVSG TALCAMVHTA
AEGVVAALEE APDRLIGTVD VLGPGERHRI LTDWNDSARE VPAATLPQLF QDRAARTPEA
PALAFGDVEL TYAELNARAN RLARLLIERG VGPESPVAVM VHRSVDLVLG FLAVTKAGGA
YLPLDPDYPA ERLAYVLDDA RPSLMLTSGD LAGRPAAAKV PLLVVDDPGT VAALREFTDT
DPVDADRRAP LLPAHPAYVI YTSGSTGRPK GVTVTHRGLP SLAAEQAEHL ALSPGSRVLQ
FASMSFDASV WETVMALCSG ACLVSAPSPE LLPGPALVRL VAERGVTHAT LPPAALALME
PDSLPSLTTL VTAGEALPRN LAAGWSAGRR LVNAYGPTES TVCATTTGPL SPEDPTTPPI
GTPIVNTGVY VLDAALRPVP VGVAGELYLA GAGLARGYLG RPGLTAERFV ANPYGGPGER
MYRTGDLARW NADGQVEYLG RTDDQVKIRG FRIELGEVES ALAAHSGVAQ SAVVVREDRP
GDRRLVGYLV AVGGPESVEV AAVRRHVQGV LPEYMVPSAL VVLDALPLTV NGKLDRKALP
APDHRAGDAG RGPATVQEEL LRTVFADVLG LPTVGMDDNF FELGGHSLLA VQLINRARTA
LGVELPMRAL FEALTVAALA QRLTDTGTAR PALTARTRPD TLPLSFAQQR LWFLGELEGP
SATYNIPVAV RLTGVLEVEA LRSALADVVG RHEVLRTVFT ADEGTPHQRI IPPSDSFELP
VTVVERSGVE RAVAELAVRA FDLAAETPLR AWLLRVAPDE HVLLMVVHHI AGDGWSMGPL
ARDVSVAYRA RVAGGVPGWN PLVVQYADYT LWQRELLGEE GDPGSVLSEQ LAFWRKALDG
VPQELALPFD RSRPAVASHR GGRVEIRVPA EVHARLVELA RAEGVTVFMV LQASLAVLLS
RMGAGSDIPV GTPVAGRTDE ALDELVGFFV NTLVMRTDLS GDPTFVELLE RVRENGLGAL
AHQDVPFERL VEDLAPARSM ARHPLFQVML ALQNTAPADL DLPGMRAEPF PTGEPPAKFD
LFLSLSETFS AASGGGGGGR PAGLLGGVDY ALDLFEHDTV QALADRFVRV LDTLTREPGR
RVGQVEVLSA EERHRVVTGW NDTAREVPAA TLPELFQAQA ARTPDTVAVA SGGAEVTYAQ
LDGRANRLAR LLVRRGIGPE DIVGLAVPRS VDLIVALLAV LKSGAGYLPI DPEYPAERIA
FMLADADPAL TLTTSAVAAR PAAGPTLLLD DPEVLAALDA LPAEAPDDGE RTTPLLPQHP
AYLIYTSGST GRPKGVVMPG GALVNLLSWH ASAVPAEPGA RVAQFTAISF DVSAQEVLSA
LLDGKTLVIP DEETRRDPEA FVTWLGEQRI TELYAPTMLL DALGEAVAER GTPLPRLRHV
AQAGEALVLG ERVRELCGEP GTVLHNHYGP SETHVVTAHT LPGDRETWPR GAAPLGRPVW
NTRVHVLDAA LRPVPVGVAG ELYVSGAQLA RGYHARPALT AQRFVASPFS PGERMYRTGD
LARWTTDGEL EYLGRTDDQV KVRGFRIELG EVEAALTAHP SVARAAAVVR EDRPGDRRLV
GYLVPADRTD GIDTAGLRAH LGARLPEHMV PSALVLLESL PLTANGKLDR RELPAPDHTA
SAAGRRPSTV QEEILCRVFA EELNVPHIGV TDNFFERGGH SLLAVRLVRR IRAVMGVDVS
VQTLMESPSV EGLAHRLSLP ATDESLRTLL PIRTHGSRPP LFCVHPRSGL SWCFAPLAAF
VPPEVPLYGL QARDADASGE LPGSIREMAE EYVAELRTVR PSGPYHLLGW SMGGRVAHEM
AVQLREDGQE VALVIMGGYA PAPEDTPGTD EAVKESAELL RRAMYERAGL DPDAPAEESD
TLDEAPDLRD LAGLPDHFRA EISKDEAAVR ARIIANNTRV YVGHRPRVFD GDVLFLSSEE
LEDGWGRAMW QPYTTGRISE ARLPCTHDEM ADPPMLPLLW NAVAAWLPTT DGDRKEEPPT
TPGAKDRSEG
//
