ID SVR3_ARATH Reviewed; 675 AA.
AC F4K410; Q93Y02; Q9FNA8;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 24-JAN-2024, entry version 90.
DE RecName: Full=Putative elongation factor TypA-like SVR3, chloroplastic;
DE AltName: Full=Protein HAPPY ON NORFLURAZON 23 {ECO:0000303|PubMed:21208309};
DE AltName: Full=Protein SUPPRESSOR OF VARIEGATION 3 {ECO:0000303|PubMed:21187014};
DE Flags: Precursor;
GN Name=SVR3; OrderedLocusNames=At5g13650 {ECO:0000312|Araport:AT5G13650};
GN ORFNames=MSH12.11 {ECO:0000312|EMBL:BAB08691.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 240-675 AND 284-675.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=21187014; DOI=10.1186/1471-2229-10-287;
RA Liu X., Rodermel S.R., Yu F.;
RT "A var2 leaf variegation suppressor locus, SUPPRESSOR OF VARIEGATION3,
RT encodes a putative chloroplast translation elongation factor that is
RT important for chloroplast development in the cold.";
RL BMC Plant Biol. 10:287-287(2010).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF ARG-438.
RX PubMed=21208309; DOI=10.1111/j.1365-313x.2010.04454.x;
RA Saini G., Meskauskiene R., Pijacka W., Roszak P., Sjoegren L.L.,
RA Clarke A.K., Straus M., Apel K.;
RT "'happy on norflurazon' (hon) mutations implicate perturbance of plastid
RT homeostasis with activating stress acclimatization and changing nuclear
RT gene expression in norflurazon-treated seedlings.";
RL Plant J. 65:690-702(2011).
CC -!- FUNCTION: Putative chloroplastic elongation factor involved in response
CC to chilling stress. Required for proper chloroplast rRNA processing
CC and/or translation at low temperature (PubMed:21187014). Involved in
CC plastid protein homeostasis (PubMed:21208309).
CC {ECO:0000269|PubMed:21187014, ECO:0000269|PubMed:21208309}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:21187014}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=F4K410-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Reduced plant growth, pale-green leaves with
CC reduced levels of chlorophyll. Chilling-sensitive phenotype.
CC {ECO:0000269|PubMed:21187014}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. BipA subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL24425.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB08691.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB006704; BAB08691.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91922.1; -; Genomic_DNA.
DR EMBL; AY059943; AAL24425.1; ALT_INIT; mRNA.
DR EMBL; BT001186; AAN65073.1; -; mRNA.
DR RefSeq; NP_851035.1; NM_180704.2. [F4K410-1]
DR AlphaFoldDB; F4K410; -.
DR SMR; F4K410; -.
DR STRING; 3702.F4K410; -.
DR PaxDb; 3702-AT5G13650-2; -.
DR ProteomicsDB; 226788; -. [F4K410-1]
DR EnsemblPlants; AT5G13650.1; AT5G13650.1; AT5G13650. [F4K410-1]
DR GeneID; 831209; -.
DR Gramene; AT5G13650.1; AT5G13650.1; AT5G13650. [F4K410-1]
DR KEGG; ath:AT5G13650; -.
DR Araport; AT5G13650; -.
DR TAIR; AT5G13650; SVR3.
DR eggNOG; KOG0462; Eukaryota.
DR HOGENOM; CLU_017016_4_0_1; -.
DR InParanoid; F4K410; -.
DR OMA; MEMAISF; -.
DR PRO; PR:F4K410; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4K410; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0050821; P:protein stabilization; IMP:UniProtKB.
DR GO; GO:0009409; P:response to cold; IMP:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; IMP:UniProtKB.
DR CDD; cd16263; BipA_III; 1.
DR CDD; cd03710; BipA_TypA_C; 1.
DR CDD; cd03691; BipA_TypA_II; 1.
DR CDD; cd01891; TypA_BipA; 1.
DR Gene3D; 3.30.70.240; -; 1.
DR Gene3D; 2.40.50.250; bipa protein; 1.
DR Gene3D; 3.30.70.870; Elongation Factor G (Translational Gtpase), domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR006298; BipA.
DR InterPro; IPR048876; BipA_C.
DR InterPro; IPR047041; BipA_GTP-bd_dom.
DR InterPro; IPR047042; BipA_II.
DR InterPro; IPR047043; BipA_III.
DR InterPro; IPR035651; BipA_V.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR042116; TypA/BipA_C.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR NCBIfam; TIGR01394; TypA_BipA; 1.
DR PANTHER; PTHR42908:SF8; TR-TYPE G DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR42908; TRANSLATION ELONGATION FACTOR-RELATED; 1.
DR Pfam; PF21018; BipA_C; 1.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SUPFAM; SSF54980; EF-G C-terminal domain-like; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Elongation factor; Plastid;
KW Protein biosynthesis; Reference proteome; Stress response; Transit peptide.
FT TRANSIT 1..58
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 59..675
FT /note="Putative elongation factor TypA-like SVR3,
FT chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000439379"
FT DOMAIN 80..275
FT /note="tr-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01059"
FT REGION 43..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 438
FT /note="R->H: In hon23; tolerance to norflurazon, an
FT inhibitor of phytoene desaturase."
FT /evidence="ECO:0000269|PubMed:21208309"
SQ SEQUENCE 675 AA; 74315 MW; A95BE1C00722140C CRC64;
MELSLSTSSA SPAVLRRQAS PLLHKQQVLG VSFASALKPG GGALRFPSRR PLPRPITCSA
SPSTAEPASE VKKKQLDRRD NVRNIAIVAH VDHGKTTLVD SMLRQAKVFR DNQVMQERIM
DSNDLERERG ITILSKNTSI TYKNTKVNII DTPGHSDFGG EVERVLNMVD GVLLVVDSVE
GPMPQTRFVL KKALEFGHAV VVVVNKIDRP SARPEFVVNS TFELFIELNA TDEQCDFQAI
YASGIKGKAG LSPDDLAEDL GPLFEAIIRC VPGPNIEKDG ALQMLATNIE YDEHKGRIAI
GRLHAGVLRK GMDVRVCTSE DSCRFARVSE LFVYEKFYRV PTDSVEAGDI CAVCGIDNIQ
IGETIADKVH GKPLPTIKVE EPTVKMSFSV NTSPFSGREG KYVTSRNLRD RLNRELERNL
AMKVEDGETA DTFIVSGRGT LHITILIENM RREGYEFMVG PPKVINKRVN DKLLEPYEIA
TVEVPEAHMG PVVELLGKRR GQMFDMQGVG SEGTTFLRYK IPTRGLLGLR NAILTASRGT
AILNTVFDSY GPWAGDISTR DLGSLVAFED GTSTSYALAS AQERGQMFVG SGVDVYKGQI
VGIHQRPGDL GLNICKKKAA TNIRSNKDVT VILDTPLTYS LDDCIEYIEE DELVEVTPSS
IRMCKNQKMA KKGRQ
//
