ID F4LSK5_TEPAE Unreviewed; 649 AA.
AC F4LSK5; L0S4B8;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN OrderedLocusNames=TEPIRE1_2446 {ECO:0000313|EMBL:CCP27294.1};
OS Tepidanaerobacter acetatoxydans (strain DSM 21804 / JCM 16047 / Re1).
OC Bacteria; Bacillota; Clostridia; Thermosediminibacterales;
OC Tepidanaerobacteraceae; Tepidanaerobacter.
OX NCBI_TaxID=1209989 {ECO:0000313|EMBL:CCP27294.1, ECO:0000313|Proteomes:UP000010802};
RN [1] {ECO:0000313|Proteomes:UP000010802}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Re1 {ECO:0000313|Proteomes:UP000010802};
RX PubMed=23469343; DOI=10.1128/genomeA.00213-12;
RA Manzoor S., Bongcam-Rudloff E., Schnurer A., Muller B.;
RT "First genome sequence of a syntrophic acetate-oxidizing bacterium,
RT Tepidanaerobacter acetatoxydans strain Re1.";
RL Genome Announc. 1:E00213-E00213(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
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DR EMBL; HF563609; CCP27294.1; -; Genomic_DNA.
DR RefSeq; WP_013779314.1; NC_019954.2.
DR AlphaFoldDB; F4LSK5; -.
DR STRING; 1209989.TepRe1_2276; -.
DR KEGG; tae:TepiRe1_2446; -.
DR KEGG; tep:TepRe1_2276; -.
DR PATRIC; fig|1209989.3.peg.2812; -.
DR eggNOG; COG1048; Bacteria.
DR HOGENOM; CLU_006714_2_3_9; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000010802; Chromosome.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR006250; Aconitase_put.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01342; acon_putative; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 2.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000010802}.
FT DOMAIN 8..284
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 283..407
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 512..577
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 649 AA; 71263 MW; 4522F215FDF0F653 CRC64;
MNKSMTRKII EDHYISGNMV AGEEVAIRID HTLTHDVTGT QAYLAFETLG IPRVKTEKSV
SYIDHNLLYT DNKNMDDHLY LQSIAKKYGL YLSRAGNGIC HIVHLERFGI PGKTLLGSDS
HTPTGGAIGM LSIGAGGMDV AMAMAGEPLY IKMPYIINVN LKGKLRGGVS AKDIILEMLR
RIGVKGGLGK VFEYTGQGLD CLSVYDRATI ANMGAEMGAT TSIFPSDETV HKFFTHQKRE
ADWVELYPDK DAYYDGVIEI DLNTLEPMVA KPHMPDNVVK VSELKDVKVN QVFIGSCTNA
SYTDFVKAAK IMENKVVHDD VSLSIAPGSR QIFSMLLRDG IISKLVASGA RVLECGCGPC
VGIGQAPNTG GISLRTSNRN FRGRGGTLDA YLYLASPEVA AATALTGYIT DPRDVIDSKL
LENIEEPREY IINDNMVINP IETNENIEII RGPNIKPMPV NDPMEEIIEA RVVAKFGDNI
TTDDIIPASA QFSALRSNIP AISEITFGRI DPGFYSRAKE YKKSIIIGGE NYGQGSSREH
AAIAPMYLGV KAVIAKSMAR IHKNNLVNHG VIPLLFADSA AYDSIEQGDC LYIEDAISQI
RTKKVKILNK TKNTFFETIV DLTDREVELI IAGGRLRFIQ NKSKINKHY
//
