ID F4RFK7_MELLP Unreviewed; 765 AA.
AC F4RFK7;
DT 28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT 28-JUN-2011, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 {ECO:0000256|ARBA:ARBA00040398};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
DE AltName: Full=Pre-mRNA-splicing ATP-dependent RNA helicase prp28 {ECO:0000256|ARBA:ARBA00039685};
GN ORFNames=MELLADRAFT_34708 {ECO:0000313|EMBL:EGG08910.1};
OS Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) (Poplar leaf
OS rust fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Melampsoraceae; Melampsora.
OX NCBI_TaxID=747676 {ECO:0000313|Proteomes:UP000001072};
RN [1] {ECO:0000313|Proteomes:UP000001072}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=98AG31 / pathotype 3-4-7 {ECO:0000313|Proteomes:UP000001072};
RX PubMed=21536894; DOI=10.1073/pnas.1019315108;
RA Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E.,
RA Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L.,
RA Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E.,
RA Goldberg J., Grabherr M.G., Kodira C.D., Kohler A., Kuees U.,
RA Lindquist E.A., Lucas S.M., Mago R., Mauceli E., Morin E., Murat C.,
RA Pangilinan J.L., Park R., Pearson M., Quesneville H., Rouhier N.,
RA Sakthikumar S., Salamov A.A., Schmutz J., Selles B., Shapiro H.,
RA Tanguay P., Tuskan G.A., Henrissat B., Van de Peer Y., Rouze P.,
RA Ellis J.G., Dodds P.N., Schein J.E., Zhong S., Hamelin R.C.,
RA Grigoriev I.V., Szabo L.J., Martin F.;
RT "Obligate biotrophy features unraveled by the genomic analysis of rust
RT fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011).
CC -!- SUBUNIT: Component of the U5 snRNP complex.
CC {ECO:0000256|ARBA:ARBA00038719}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX23/PRP28
CC subfamily. {ECO:0000256|ARBA:ARBA00037954}.
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DR EMBL; GL883099; EGG08910.1; -; Genomic_DNA.
DR RefSeq; XP_007407884.1; XM_007407822.1.
DR AlphaFoldDB; F4RFK7; -.
DR STRING; 747676.F4RFK7; -.
DR EnsemblFungi; EGG08910; EGG08910; MELLADRAFT_34708.
DR GeneID; 18927410; -.
DR KEGG; mlr:MELLADRAFT_34708; -.
DR VEuPathDB; FungiDB:MELLADRAFT_34708; -.
DR eggNOG; KOG0333; Eukaryota.
DR HOGENOM; CLU_003041_11_3_1; -.
DR InParanoid; F4RFK7; -.
DR OrthoDB; 5487240at2759; -.
DR Proteomes; UP000001072; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd17945; DEADc_DDX23; 1.
DR CDD; cd18787; SF2_C_DEAD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR PANTHER; PTHR47958; ATP-DEPENDENT RNA HELICASE DBP3; 1.
DR PANTHER; PTHR47958:SF56; RNA HELICASE; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU000492};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU000492};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000492};
KW mRNA processing {ECO:0000256|ARBA:ARBA00023187};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU000492};
KW Reference proteome {ECO:0000313|Proteomes:UP000001072}.
FT DOMAIN 308..336
FT /note="DEAD-box RNA helicase Q"
FT /evidence="ECO:0000259|PROSITE:PS51195"
FT DOMAIN 339..551
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 562..724
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 69..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 308..336
FT /note="Q motif"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00552"
FT COMPBIAS 69..96
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..123
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 765 AA; 83814 MW; B0D411898673BBA4 CRC64;
MGPSPAVKAA AGVLISSSSS STLRTNGNKQ PLSIESLLAQ QKAEKEAASK PKFLSKEERA
TLAIEKRAQE VAAEKARLAE AAAKRDELER SARESKYGSY STDATDSSST SNNAASNSMP
PPNFPASASL PDMKGNSVLP NNGPMLGTAP PPALNQKLLM ARYLGQPDNK KRRIRKMSDK
KFVFDWAKDE DTALEEVDPL YAVTAPPISA PIVGRFGLYG NGKLAGIDPA AQSVRNHSRT
TYQTRYNPDD EVLTSTPMST GSKRSSAVIN ELHWSQKPLE AMRERDWRIF REDFSIAARG
GNIPNPMRSW DESKIPMQIL EIIDEVGYKE PSPIQRQAIP LGLNNRDLIG IAETGSGKTA
SFVIPMLTYI GKLAPLTDEN RHLGPYALIL APTRELAQQI ESETNKFALR LGYRCVSIVG
GKAMEEQALN MRDGAEIIIA TPGRLKDCIE RHVLVLGQCT YVVMDEADRM INLGFEEVVN
FILDQLPLSN LKPDTEEAED SAKMTSFIGG IEGFDVSGIK GLYRQTVMFS ATMPPAVERL
ARKYLRRPAV VTIGVAGQAV DTVDQQVEFL PNEDKKRGRL LEVLNQGHTP PIIVFVNQKK
TADQLAKDIS RAGWSTTTLH SGKNQEQREA ALASLRAGES DILVATDLAG RGIDVPNVSL
VVNFQMAGTI EAYVHRIGRT GRAGKVGTAI TFLTNDDADV LYDLKQEITK SPVSRCPPEL
AKHEAAQSKV RYHLFTSRVI DTEVSIFFSS KLLDVCRHET KGRRS
//
